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pCF826-sgCIDE-1_U6-sgRNA-EFS-Cas9-P2A-mCherry2

Plasmid

#211690

Purpose

U6-sgRNA-EFS-Cas9-P2A-mCherry2

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-1 guide RNA vector

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCF826-sgCIDE-2_U6-sgRNA-EFS-Cas9-P2A-mCherry2

Plasmid

#211691

Purpose

U6-sgRNA-EFS-Cas9-P2A-mCherry2

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-2 guide RNA vector

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH1-CMV-FIP200-L324D/R543D/R548D-mCherry-Hygro

Plasmid

#210856

Purpose

Lentiviral expression vector encoding FIP200 mutant-mCherry

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

FIP200 (RB1CC1 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mutation

L324D/R543D/R548D

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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psiCheck2-SV40p-5'UTR-mtIF3(SR)-3xFLAG-3'UTR-HSVTKp-mCherry

Plasmid

#182379

Purpose

Dual expression construct encoding shRNA-resistant mtIF3 and mCherry from separate promoters

Depositor

Chunghun Lim

Article

Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

Inserts

mtIF3 (Mtif3 Mouse)
mCherry

Use

Tags

3xFlag

Expression

Mammalian

Mutation

Changed CDS sequence from 'ccacgttcaagtcacga…

Promoter

HSV TK promoter and SV40 promoter

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pSL006_phiC31_neo-5xTetO-pEF-H2B-mCitrine-pRSV-H2B-mCherry

Plasmid

#179425

Purpose

Dual fluorescent reporter construct with no spacer, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration

Depositor

Lacra Bintu

Article

Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

Insert

TRE-cit-(nospacer)-mch

Use

Tags

Expression

Mammalian

Mutation

Promoter

pEF alpha, pRSV

Available since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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PylT(AGGA)Ev2 PylS mCherry-P2A-eGFP(AGGA)

Plasmid

#174528

Purpose

Dual-fluorescence reporter for quantifying PylT(AGGA)Ev2 decoding efficiency

Depositor

Yu-Hsuan Tsai

Article

Mills et al Cell Rep Methods. 2021 Sep 16;1(6):10007

Insert

PylS

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Neurog2 x2)

Plasmid

#171100

Purpose

CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Neurog2.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9 (Neurog2 S. pyogenes, Mouse)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Ef1a

Available since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Ascl1 x2)

Plasmid

#171099

Purpose

CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Ascl1.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9 (Ascl1 S. pyogenes, Mouse)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Ef1a

Available since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pRDAX-571 C-Tag GFP[2] L10 Stat3(R609Q) mCherry

Plasmid

#130118

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Stat3(R609Q)

Use

Tags

Expression

Mammalian

Mutation

R609Q

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-572 N-Tag GFP[2] L10 Stat3(R609Q) mCherry

Plasmid

#130119

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Stat3(R609Q)

Use

Tags

Expression

Mammalian

Mutation

R609Q

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-573 C-Tag GFP[2] L20 Stat3(R609Q) mCherry

Plasmid

#130120

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Stat3(R609Q)

Use

Tags

Expression

Mammalian

Mutation

R609Q

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDAX-574 N-Tag GFP[2] L20 Stat3(R609Q) mCherry

Plasmid

#130121

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Stat3(R609Q)

Use

Tags

Expression

Mammalian

Mutation

R609Q

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDAX-576 N-Tag GFP[2] L32 Stat3(R609Q) mCherry

Plasmid

#130123

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Stat3(R609Q)

Use

Tags

Expression

Mammalian

Mutation

R609Q

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDAX-620 N-Tag Venus210[2] L10 Stat3(R609Q) mCherry

Plasmid

#130095

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Stat3(R609Q)

Use

Tags

Expression

Mammalian

Mutation

R609Q

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDAX-621 C-Tag Venus210[2] L20 Stat3(R609Q) mCherry

Plasmid

#130096

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Stat3(R609Q)

Use

Tags

Expression

Mammalian

Mutation

R609Q

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDAX-622 N-Tag Venus210[2] L20 Stat3(R609Q) mCherry

Plasmid

#130097

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Stat3(R609Q)

Use

Tags

Expression

Mammalian

Mutation

R609Q

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDAX-623 C-Tag Venus210[2] L32 Stat3(R609Q) mCherry

Plasmid

#130098

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Stat3(R609Q)

Use

Tags

Expression

Mammalian

Mutation

R609Q

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDAX-624 N-Tag Venus210[2] L32 Stat3(R609Q) mCherry

Plasmid

#130099

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Stat3(R609Q)

Use

Tags

Expression

Mammalian

Mutation

R609Q

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDAX-524 N-Tag GFP[1] L10 Tln1(rod) mCherry

Plasmid

#130051

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Tln1 rod

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDAX-525 C-Tag GFP[1] L20 Tln1(rod) mCherry

Plasmid

#130052

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Tln1 rod

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge