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Plasmid#188770PurposedCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)DepositorInsertdCas9-XTEN80-KRAB(Kox1)
UseLentiviralTagsHA-2xNLS-XTEN80(linker)-KRAB(Kox1)ExpressionMutationPromoterSFFVAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHBS1335 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G295S]
Plasmid#107848PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationG295S in splicing reporterPromoterAvailable sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
EK0387 SFFV-mTagBFP2-SGc(Bdnf.s1)cSG-mCherry (FLP-IN)
Plasmid#191157PurposeExpression of a sensor for murine Bdnf 3' UTR in mammalian cells; mTagBFP2 marker, mCherry output; 90 bp longDepositorInsertmTagBFP2:Bdnf.s1:mCherry
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Olig2 x2)
Plasmid#171101PurposeCRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Olig2.DepositorInserthSpCas9 (Olig2 S. pyogenes, Mouse)
UseCRISPRTagsExpressionMutationPromoterEf1aAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRRL-pEF-H2B-mCherry-T2A-rTetR-DNMT3L-SV40
Plasmid#186967PurposeLentiviral vector encoding DNMT3A recruiter for methylation reporter (pEF-H2B-mCherry-T2A-rTetR-DNMT3L-SV40) for expression in mammalian cells.DepositorInsertDNMT3L (DNMT3L Human, Synthetic)
UseLentiviralTagsrTetR fusionExpressionMammalianMutationPromoterEF-1alpha promoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
SIN-cPPT-H1-sgHtt1-7SK-sgCas9-PGK-mCherry-WPRE
Plasmid#87912PurposeKamicas9 lentiviral vectorDepositorInsertshHTT1, sgCas9, cherry
UseLentiviralTagsExpressionMutationPromoterH1, 7SK, PGKAvailable sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Otx2 x2)
Plasmid#171102PurposeCRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Otx2.DepositorInserthSpCas9 (Otx2 S. pyogenes, Mouse)
UseCRISPRTagsExpressionMutationPromoterEf1aAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSEM234 - pEXP[Pmlc-1 | mCherry(2xNLS) | cbr-tbb-2 UTR]
Plasmid#159795PurposeFluorescent co-injection marker to identify extrachromosomal arrays in C. elegansDepositorInsertpEXP[Pmlc-1 | mCherry(2xNLS) | cbr-tbb-2 UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
4xPylT(AGGA)Ev2 PylS mCherry-P2A-eGFP(AGGA)
Plasmid#174529PurposeDual-fluorescence reporter for quantifying PylT(AGGA)Ev2 decoding efficiencyDepositorInsertsPylS
PylT
UseTagsFlagExpressionMammalianMutationPromoterU6Available sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-619 C-Tag Venus210[2] L10 Stat3(R609Q) mCherry
Plasmid#130094PurposePPI (BiFC/TriFC) assay vectorDepositorInsertStat3(R609Q)
UseTagsExpressionMammalianMutationR609QPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCru5-ACC/ACC/ACC/ACC-mEGFP-IRES-mCherry
Plasmid#49218PurposeRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseRetroviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianMutationPromoterViral LTR (or CMV)Available sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHBS1338 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G348V]
Plasmid#107852PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationG348V in splicing reporterPromoterAvailable sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHBS1398 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G368W]
Plasmid#118811PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationG368W in CTD of full-length TDP43PromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1285 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G309F]
Plasmid#107851PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationG309F in splicing reporterPromoterAvailable sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHBS1202 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 4xΦ]
Plasmid#107845PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationAll hydrophobics combined in four clusters in spl…PromoterAvailable sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHBS1203 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 2xΦ]
Plasmid#107844PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationAll hydrophobics combined in two clusters in spli…PromoterAvailable sinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHBS1339 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G348S]
Plasmid#107850PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationG348S in splicing reporterPromoterAvailable sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Myc(Δ1-143; ΔDeg1ΔDeg2)-GFP-IRES-mCherry (ΔTADΔDeg1ΔDeg2)
Plasmid#231234PurposeMYC stability reporter construct with deletion of the N-terminal region (residues 1-143) and point mutations corresponding to the deletion of degron 1 and 2 for transient expression in mammalian cellsDepositorInsertMYC (MYC Human)
UseTagsExpressionMammalianMutationTAD deleted (Δ1-143), deletion of degron 1 & …PromoterAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ E4F1 degron(220-242)-GFP-IRES-mCherry
Plasmid#231007PurposeProtein stability reporter construct for E4F1 consisting of aa 220-242 for transient overexpression in mammalian cells.DepositorInsertE4F1 aa220-242 (E4F1 Human)
UseTagsAcGFP1ExpressionMammalianMutationPromoterAvailable sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ GFP-CDK5R1 degron (283-307)-IRES-mCherry
Plasmid#231008PurposeProtein stability reporter construct for CDK5R1 consisting of aa 283-307 for transient overexpression in mammalian cells.DepositorInsertCDK5R1 aa283-307 (CDK5R1 Human)
UseTagsAcGFP1ExpressionMammalianMutationPromoterAvailable sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only