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pTH733-2µ-RLuc/maxCFLuc
Plasmid#40608DepositorInsertsFirefly Luciferase
Renilla luciferase
UseTagsExpressionYeastMutationAll codons of the original FLuc gene were exchang…PromoterADH1 and TDH3Available sinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.1-CCND1
Plasmid#172632PurposeExpresses untagged cyclin D1 in mammalian cellsDepositorInsertCCND1 (CCND1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-mCherry.3xFLAG.NOS1AP396-500-WPRE
Plasmid#174134PurposepAAV plasmid expressing an mCherry.3xFLAG.NOS1AP396-500 fusion protein under the hSyn promoterDepositorInsertNos1ap (Nos1ap Mouse)
UseAAVTags3xFLAG and mCherryExpressionMutationOnly contains the sequences coding for amino acid…PromoterhSynAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDN-D2irC6kwh
Plasmid#44725DepositorInsertspCMV-D2i promoter
mCherry
UseSynthetic Biology; Expression regulator/reporterTagsRabbit β-globin intron II and WPREExpressionMammalianMutationInitiator motif (Inr) displaced relative to pCMV-…PromoterpCMV-D2iAvailable sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
EPHB1-bio-His
Plasmid#51750PurposeExpresses full-length Ephrin type-B receptor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertEPHB1 (EPHB1 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
MPL-bio-His
Plasmid#53339PurposeExpresses full-length Thrombopoietin receptor precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertNM_005373.2 (MPL Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMTB2-Avi-Cerulean-RanGAP
Plasmid#79878PurposeBeta-actin2 promoter driving N-term Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequencesDepositorInsertCerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
UseTagsAviExpressionBacterialMutationPromoterBeta-actin2Available sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
MET-bio-His
Plasmid#52022PurposeExpresses full-length Hepatocyte growth factor receptor precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertMET (MET Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3-Flt3-Y591F/Y919F
Plasmid#74497PurposeOverexpression of FLT3 Y591F/Y919F mutant in mammalian cellsDepositorInsertFLT3 (FLT3 Human)
UseTagsExpressionMammalianMutationY591F/Y919F, T227MPromoterCMVAvailable sinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
LAMP1-bio-His
Plasmid#51809PurposeExpresses full-length Lysosome-associated membrane glycoprotein 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertLAMP1 (LAMP1 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pcDNAI-GAL4-CREB-S133A
Plasmid#46770PurposeExpression of GAL4 fusion to CREB-S133ADepositorUseTagsGAL4ExpressionMammalianMutationChanged CREB serine 133 to AlaninePromoterCMVAvailable sinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pcDNAI-GAL4-CREB-S142A
Plasmid#46771PurposeExpression of GAL4 fusion to CREB-S142ADepositorUseTagsGAL4ExpressionMammalianMutationChanged CREB serine 142 to AlaninePromoterCMVAvailable sinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
-53 bp human IFN-gamma luc
Plasmid#17597DepositorInsertIFN gamma promoter (IFNG Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutation-53 to +70 of promoterPromoterAvailable sinceApril 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcU6_3 MS2 sgRNA
Plasmid#92394PurposeChick-specific U6 sgRNA expression mini-vector, harbouring chick U6_3 pol III promoter, with tracrRNA scaffold containing stem loops allowing binding of bacteriophage MS2 coat protein MCPDepositorInsertchick U6.3 promoter and gRNA cloning cassette including MS2 stem loops
UseCRISPRTagsExpressionMammalianMutationPromoterchick U6.3Available sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMT-ubb-Avi-Cerulean-Rpl10a
Plasmid#79882PurposeUbiquitin promoter driving Avi-tagged protein containing Cerulean protein fused to zebrafish Rpl10a ribosomal unit (Tryon et al. 2012); flanked by Tol2 sequencesDepositorInsertCerulean protein fused to zebrafish Rpl10a ribosomal unit (rpl10a Zebrafish)
UseTagsAviExpressionBacterialMutationPromoterUbiquitinAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only
