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pDD287 (mKate2^SEC^3xMyc
Plasmid#70685PurposemKate2^SEC^3xMyc vector with ccdB sites for cloning homology armsDepositorInsertmKate2-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xMyc and C. elegans codon-optimized mKate2ExpressionWormMutationPromoterAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBBK05 pCAS-Tyr-[gRNA: BsaI GFP dropout] (NatR,AmpR)
Plasmid#178989PurposeSp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Nourseothricin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P05
Plasmid#120016PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with eGFPDepositorInserteGFP
UsePcr templateTagseGFPExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-FLEX-TVA-mCherry
Plasmid#38044DepositorInsertAvian sarcoma and leukemia virus receptor 950 (CD320 Synthetic, quail)
UseAAV and Cre/LoxTagsmCherryExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV SOCS-3
Plasmid#11486DepositorInsertSOCS-3 (Socs3 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceApril 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAJT430
Plasmid#202291PurposeRPU integration plasmid (LPattB7)DepositorInsertJ23101-RiboJ00-B0034-YFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
Ca-FLARE (TF)
Plasmid#92213PurposeTranscription factor component of FLAREDepositorInsertNRX-TM-Nav1.6 -CaMbp(M2)-eLOV-TEVcs(ENLYFQ▲M)-tTA-VP16
UseAAVTagsExpressionMammalianMutationPromotersynapsinAvailable sinceJune 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
2xP53_RE::NLuc
Plasmid#124533Purposep53 NLuc luciferase reporterDepositorInsertP53 NLuc reporter
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-EGFP-NLS
Plasmid#165441PurposeExpresses nuclear EGFP from CMV promoterDepositorInsertEGFP-NLS
UseAAVTagsNLSExpressionMammalianMutationPromoterCMVAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-phoAp-GFPuv
Plasmid#139072PurposeLow phosphate inducible promoter phoAp for GFP expressionDepositorInsertphoAp-GFPuv
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSSM16: pCMV-HA-NLS-SpuFz1
Plasmid#205271PurposeHuman expression vector for human codon optimized N-terminal NLS-tagged SpuFz1DepositorInsertSpuFz1
UseTagsHA, NLS(Makkerh)ExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
Pdgfra-T2A-H2B-eGFP donor
Plasmid#113120PurposeDonor plasmid for knock-in T2A-H2B-eGFP to the c-terminal of mouse Pdgfra coding sequenceDepositorInsertPdgfra donor region including homology arms and T2A-H2B-eGFP (Pdgfra Synthetic, Mouse)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMJ548: pU6-SpuFz1_MS2-omegaRNA(75nt)-entry
Plasmid#205269PurposeHuman expression vector for SpuFz1 omegaRNA 75-nt scaffold + 5' MS2- (BbsI guide entry site)DepositorInsertSpuFz1 omegaRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV::TVA/tRFP
Plasmid#164486PurposeDual CMV promoter plasmid to express TVA and TurboRFPDepositorInsertsUseTagsExpressionMammalianMutationPromotersCMV and sCMV IE94Available sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
MStart-36xMashTag-BFP-3xStop-24xPP7 (Moonstart-MashTag reporter)
Plasmid#128607PurposeExpresses 36xMashTag with MoonTag frame as mainframe, followed by BFP linker and 24 repeats of PP7 hairpins in 3’ UTR.DepositorInsert36xMashTag
UseTagsExpressionMammalianMutationPromoterPcmvAvailable sinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMJ529: pU6-SpuFz1_MS2-omegaRNA(75nt)-Guide(B2M)
Plasmid#205278PurposeHuman expression vector for SpuFz1 omegaRNA 75-nt scaffold + 5' MS2- (Guide:B2M)DepositorInsertSpuFz1 omegaRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
3xDBE_RE::Renilla
Plasmid#124535PurposeFoxo Renilla luciferase reporterDepositorInsertFOXO Renilla reporter
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA
Plasmid#50943PurposeBicistronic vector expressing mRuby2 and GCaMP6f from a single open reading frame.DepositorInsertmRuby2-P2A-GCaMP6f
UseAAVTagsExpressionMutationPromoterhSyn1Available sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-N1-eKR2
Plasmid#115337PurposeExpresses enhanced sodium pump for light-driven extrusion of sodium; activation with green light; codon-optimized for human/mouseDepositorInserteKR2
UseTagsEYFPExpressionMammalianMutationnonePromoterCMVAvailable sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only
