Addgene

We narrowed to 27,415 results for: eng

Showing: 3801 - 3820 of 27415 results

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pGGB003

Plasmid

#48821

Purpose

Provides a default dummy as GreenGate N-terminal tag module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

B-Dummy

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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TH mAb_BCN53.1.1D10_LC_Mouse Ig Kappa

Plasmid

#182897

Purpose

Mammalian expression of TH mouse mAb LC gene

Depositor

Heng Zhu

Insert

anti-TH (human) light chain (TH Mouse)

Use

Affinity Reagent/ Antibody

Tags

Expression

Mammalian

Mutation

No

Promoter

CMV

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-miR30

Plasmid

#20670

Depositor

Bryan Cullen

Article

Zeng et al Mol Cell. 2002 Jun . 9(6):1327-33.

Insert

miR-30 (MIR30A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 5, 2009

Availability

Academic Institutions and Nonprofits only
pSFFV_sfCherry2(11)_TagBFP

Plasmid

#82606

Purpose

Expresses sfCherry2(11) and TagBFP fusion in mammalian cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

sfCherry2(11)_TagBFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

changed Gly12 to Ala in sfCherry11+

Promoter

SFFV

Available since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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pSEVA6213S

Plasmid

#122094

Purpose

constitutive expression of I-SceI for genome engineering in Pseudomonas

Depositor

Pablo Ivan Nikel

Article

Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Insert

I-SceI restriction endonuclease

Use

Tags

Expression

Mutation

Promoter

Pem7

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pGGD002

Plasmid

#48834

Purpose

Provides a default dummy as GreenGate C-terminal tag module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

D-Dummy

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL12006

Plasmid

#50270

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter + 5'UTR (Cassava Vein Mosaic Virus)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pGGA006

Plasmid

#48816

Purpose

Provides the UBQ10 promoter as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

UBQ10 promoter

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_PAsfCherry2(1-10)

Plasmid

#82612

Purpose

Express PAsfCherry2(1-10) in mammalian cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

PAsfCherry2(1-10)

Use

Tags

Expression

Mammalian

Mutation

changed Glu 118 to Gln, changed Thr 128 to Ile

Promoter

CMV

Available since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pAC1812_pCR8-gCasRx-CUG (control gRNA)

Plasmid

#118645

Purpose

Transient transfection; Expressed Control (CUG repeat) CasRx gRNA; Gateway Donor

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

gCasRx-CUG

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pICSL30009

Plasmid

#50301

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

N terminal Myc tag (4x Myc)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pFlare5GdP

Plasmid

#90250

Purpose

Minigene reporter for alternative splicing analysis

Depositor

Douglas Black

Article

Zheng et al Genome Res. 2013 Jun;23(6):998-1007. doi

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pGGE009

Plasmid

#48841

Purpose

Provides the UBQ10 terminator as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

UBQ10 terminator

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL50008

Plasmid

#50314

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, GFP (A. victoria)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only

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pET28a-PAN

Plasmid

#200971

Purpose

Express poly-histidine tagged Methanococcus jannaschii PAN in E. coli recombinantly

Depositor

Yifan Cheng

Article

Yu et al Nat Commun. 2020 Sep 11;11(1):4580. doi:

Insert

PAN

Use

Tags

6xHis and 6xHis-thrombin site

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pICSL50009

Plasmid

#50309

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

C terminal HA tag (6x Human influenza hemagglutinin)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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PEG-Actinin-C-sstFRET

Plasmid

#61101

Purpose

Expression of α-actinin with C-term sstFRET inserted to measure cytoskeletal stress in vivo

Depositor

Fred Sachs

Article

Meng et al J Cell Sci. 2011 Jan 15;124(Pt 2):261-9.

Insert

α-actinin-sstFRET

Use

Tags

sstFRET

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pGGD003

Plasmid

#48835

Purpose

Provides linker:mCherry as GreenGate C-terminal tag module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

Linker-mCherry

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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Ai38

Plasmid

#34883

Depositor

Hongkui Zeng

Article

Zariwala et al J Neurosci. 2012 Feb 29;32(9):3131-41. d

Insert

CAG-Floxed GCaMP3

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

CAG

Available since

March 7, 2012

Availability

Academic Institutions and Nonprofits only

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A3RXB7

Plasmid

#177694

Purpose

expresses a N-acetyl hexosamine oxidase in E. coli

Depositor

Martin Engqvist

Article

Rembeza et al Chembiochem. 2022 Jan 19;23(2):e20210051

Insert

N-acetylhexosamine oxidase

Use

Tags

10xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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