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pDEST-CMV 3xFLAG-GABARAPL1-GFP

Plasmid

#123112

Purpose

Expresses 3xFLAG-GABARAPL1-EGFP in mammalian cells, for monitoring ATG4 activity towards GABARAPL1

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)

Use

Tags

3xFLAG and EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-SNAP-CRY2-VHH(GFP)

Plasmid

#58370

Purpose

For use in light-induced inactivation of GFP fused protein through the interaction with CIB1-MP

Depositor

Won Do Heo

Article

Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

Insert

CRY2[1-498] (CRY2 Mustard Weed)

Use

Tags

SNAP and vhhGFP4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV6M-PAK1 H83L H86L K299R

Plasmid

#26592

Depositor

Jonathan Chernoff

Article

Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

Insert

Pak1 (PAK1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

H83L H86L K299R Dominant negative

Promoter

Available since

March 9, 2011

Availability

Academic Institutions and Nonprofits only
pCMV-EGFP-T2A-ACREB-WPRE

Plasmid

#40867

Depositor

Harold Gainer

Article

Lubelski et al Brain Res. 2012 Jan 6;1429:18-28. Epub 2

Insert

ACREB

Use

AAV

Tags

Expression

Mutation

Promoter

CMV

Available since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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CMV:β2Ar-hLOV-TEVcs(ENLYFQ/M)-GAL4-V5

Plasmid

#135458

Purpose

Mammalian expression of SPARK TF component

Depositor

Alice Ting

Article

Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

Insert

β2AR-hLOV-TEVcs(ENLYFQ/M)-GAL4-V5

Use

Lentiviral

Tags

Flag, V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-ND1 Left -GSVG-UGI

Plasmid

#187412

Purpose

human mitochondrial DNA ND1 target TALE

Depositor

Jin-Soo Kim

Article

Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

Insert

DddA GSVG variant

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-BiP-Myc-KDEL T37G

Plasmid

#27165

Depositor

Ron Prywes

Article

Shen et al Dev Cell. 2002 Jul . 3(1):99-111.

Insert

BiP (HSPA5 Human)

Use

Tags

KDEL and Myc

Expression

Mammalian

Mutation

ATP bound form: T37G

Promoter

Available since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only
pTRIP-CMV-mTagBFP2-2A-FLAG-ntcGAS

Plasmid

#102603

Purpose

Lentivector to express Flag-tagged and shRNA-resistant cGAS and mTagBFP

Depositor

Nicolas Manel

Article

Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

Insert

cGAS (CGAS Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_3C-Twin-Strep

Plasmid

#113883

Purpose

Mammalian (inducible) protein expression

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Twin-Strep

Expression

Mutation

Promoter

CMV-MIE-TetO2

Available since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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CMV-mEGFP(A206K)-paCaMKII(T286A)

Plasmid

#165439

Purpose

Expresses photoactivatable CaMKII(T286A: Autophosphorylation deficient) in mammalian cells to induce synaptic plasticity.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

mEGFP(A206K)-paCaMKII(T286A)

Use

Tags

mEGFP(A206K)

Expression

Mammalian

Mutation

T286A: Autophosphorylation deficient mutation

Promoter

CMV

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti CMV-IFP2.0 T2A HO1

Plasmid

#59427

Purpose

Express infrared fluorescent protein IFP2.0 plus HO1 enzyme

Depositor

Xiaokun Shu

Article

Yu et al Nat Commun. 2014 May 15;5:3626. doi: 10.

Insert

IFP2.0T2AHO1 IRES EGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV 3xFLAG-GABARAP-GFP

Plasmid

#123111

Purpose

Expresses 3xFLAG-GABARAP-EGFP in mammalian cells, for monitoring ATG4 activity towards GABARAP

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)

Use

Tags

3xFLAG and EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-TM-CIBN-BLITz1-TetR-VP16

Plasmid

#89878

Purpose

expresses TM-CIBN-BLITz1-TetR-VP16 of BLITz system

Depositor

Hyungbae Kwon

Article

Lee et al Nat Methods. 2017 May;14(5):495-503. doi

Insert

TM-CIBN-TevN-BLITz1-TetR-VP16

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV.CMV.SV40.THBS1-deltaTSR1-HA.SV40(polyA)

Plasmid

#156162

Purpose

Expresses murine Thrombospondin-1 (THBS1) with deletion (AA 383-548) and HA-tag at C-terminus

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Thrombospondin-1 (Thbs1 Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Mutation

THBS1 with deletion of amino acid residues 383-548

Promoter

CMV

Available since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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p1553 CMV-EBNA1 (delta GGA)

Plasmid

#26648

Depositor

Bill Sugden

Article

Aiyar et al J Biol Chem. 1998 Dec 4. 273(49):33073-8

Insert

Epstein-Barr Nuclear Antigen 1 (EBNA-1 Epstein-Barr Virus)

Use

Tags

Expression

Mammalian

Mutation

deletion of 224 amino acids of EBNA-1 (aa101-324 …

Promoter

Available since

July 10, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgCnr1

Plasmid

#209196

Purpose

Mutagenesis of Cnr1

Depositor

Larry Zweifel

Article

Gunduz-Cinar et al Neuron. 2023 Jul 19:S0896-6273(23)00482-

Insert

Cnr1 (Cnr1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

CMV

Available since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV 3xFLAG-LC3A-GFP

Plasmid

#123106

Purpose

Expresses 3xFLAG-LC3A-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3A

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)

Use

Tags

3xFLAG and EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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p7058 pHAGE-P-CMVt-N-HA-GAW-FDFT1

Plasmid

#100151

Purpose

Expresses FDFT1

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

FDFT1 (FDFT1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-AsCas12a-P2A-EGFP (RTW2861)

Plasmid

#160140

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AsCas12a with a c-terminal NLS(nucleoplasmin), 3x HA tag, and P2A-EGFP

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Insert

human codon optimized AsCas12a with NLS(nucleoplasmin)-3xHA-P2A-EGFP

Use

In vitro transcription; t7 promoter

Tags

NLS(nucleoplasmin)-3xHA-P2A-EGFP

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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p6899 PHAGE-P CMVT N-HA-hE6AP II C843A

Plasmid

#37604

Depositor

Peter Howley

Article

Martinez-Noel et al Mol Cell Biol. 2012 May 29.

Insert

E6AP II C843A (UBE3A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

C843A

Promoter

CMVt

Available since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only