Addgene

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pTU-A-000

Plasmid

#124409

Purpose

Slot A, empty (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot A + terminator + spacer

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRAV24

Plasmid

#67716

Purpose

Parental plasmid for transcription of RNAs with a GlmS/M-domain 3'-tag for native purification

Depositor

Jeffrey Kieft, Robert Batey

Article

Batey et al RNA. 2007 Aug;13(8):1384-9. Epub 2007 Ju

Insert

Transcription cassette for 3'-tagged RNAs

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 RNA polymerase

Available since

Sept. 8, 2015

Availability

Academic Institutions and Nonprofits only
pC-0_v4

Plasmid

#124424

Purpose

Full MCS, lacks araC & P(BAD) (step 2)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
TarsierMER20MER39

Plasmid

#35261

Depositor

Gunter Wagner

Article

Emera et al Proc Natl Acad Sci U S A. 2012 Jul 10;10

Insert

MER20/MER39

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

March 20, 2012

Availability

Academic Institutions and Nonprofits only
orangutanMER20MER39

Plasmid

#35257

Depositor

Gunter Wagner

Article

Emera et al Proc Natl Acad Sci U S A. 2012 Jul 10;10

Insert

MER20/MER39

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

March 19, 2012

Availability

Academic Institutions and Nonprofits only
colobusMER20MER39

Plasmid

#35258

Depositor

Gunter Wagner

Article

Emera et al Proc Natl Acad Sci U S A. 2012 Jul 10;10

Insert

MER20/MER39

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

March 19, 2012

Availability

Academic Institutions and Nonprofits only
AtelesMER20MER39

Plasmid

#35259

Depositor

Gunter Wagner

Article

Emera et al Proc Natl Acad Sci U S A. 2012 Jul 10;10

Insert

MER20/MER39

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

March 19, 2012

Availability

Academic Institutions and Nonprofits only
CebusMER20MER39

Plasmid

#35260

Depositor

Gunter Wagner

Article

Emera et al Proc Natl Acad Sci U S A. 2012 Jul 10;10

Insert

MER20/MER39

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

March 19, 2012

Availability

Academic Institutions and Nonprofits only
pTarget_CAST

Plasmid

#127926

Purpose

Target plasmid containing PSP1

Depositor

Feng Zhang

Article

Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

Insert

PSP1 target (GGTT PAM)

Use

Tags

Expression

Mutation

Promoter

Available since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mCherry

Plasmid

#128744

Purpose

Expresses mCherry in mammalian cells

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSynapsin-Flpo

Plasmid

#60663

Purpose

Expresses pAAV-hSynapsin-Flpo

Depositor

Massimo Scanziani

Article

Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

Insert

Flpo

Use

AAV

Tags

Expression

Mutation

Promoter

hSynapsin1

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-RILP

Plasmid

#110498

Purpose

Fluorescent RILP

Depositor

Bernardo Mainou, Terence Dermody

Article

Mainou et al J Virol. 2012 Aug;86(16):8346-58. doi: 1

Insert

RILP (RILP Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAC-Luc

Plasmid

#33154

Depositor

Michael Rosbash

Article

Kadener et al Genes Dev. 2007 Jul 1;21(13):1675-86. Ep

Type

Empty backbone

Use

Luciferase

Tags

Luciferase

Expression

Insect

Mutation

Promoter

Actin 5C

Available since

Dec. 8, 2011

Availability

Academic Institutions and Nonprofits only
pAAV-Ptet-RFP-shR-rtTA

Plasmid

#35625

Depositor

Howard Gu

Article

Naughton et al Anal Biochem. 2011 Oct 1;417(1):162-4. E

Type

Empty backbone

Use

AAV and RNAi; Tet inducible

Tags

Expression

Mammalian

Mutation

Promoter

Ptet

Available since

April 19, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pFp10

Plasmid

#170460

Purpose

EarlyBac insect cell expression system

Depositor

Hiro Furukawa

Article

Chou et al Cell. 2020 Jul 23;182(2):357-371.e13. do

Insert

none

Use

Tags

Expression

Insect

Mutation

Promoter

dHsp70

Available since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMAL-c2X

Plasmid

#75286

Purpose

Expresses proteins in the cytoplasm as fusions to maltose-binding protein

Depositor

Paul Riggs

Article

Walker et al Appl Microbiol Biotechnol. 2010 Sep;88(1

Type

Empty backbone

Use

Tags

maltose-binding protein

Expression

Bacterial

Mutation

Promoter

tac

Available since

July 18, 2016

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
EGFP-Rab13

Plasmid

#110500

Purpose

Fluorescent Rab13

Depositor

Bernardo Mainou

Article

Mainou et al J Virol. 2012 Aug;86(16):8346-58. doi: 1

Insert

Rab13 (RAB13 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSPLp10

Plasmid

#170462

Purpose

EarlyBac insect cell expression system

Depositor

Hiro Furukawa

Article

Chou et al Cell. 2020 Jul 23;182(2):357-371.e13. do

Insert

New vector

Use

Tags

Expression

Insect

Mutation

Promoter

dHsp70

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
R5 PV

Plasmid

#51734

Purpose

Dicistronic vector with PV IRES

Depositor

Vincent Mauro

Article

Burman et al Nucleic Acids Res. 2012 Sep;40(16):8085-

Insert

PV IRES

Use

Tags

Expression

Mutation

Promoter

SV40

Available since

July 14, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-mNaChBac-T2A-tdTomato

Plasmid

#60650

Purpose

Expresses mNaChBac-T2A-tdTomato

Depositor

Massimo Scanziani

Article

Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

Insert

mNaChBac-T2A-tdTomato

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
R5 EMCV

Plasmid

#51733

Purpose

Dicistronic vector with EMCV IRES

Depositor

Vincent Mauro

Article

Burman et al Nucleic Acids Res. 2012 Sep;40(16):8085-

Insert

EMCV IRES

Use

Tags

Expression

Mutation

Promoter

Available since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pHMM

Plasmid

#67717

Purpose

Expression vector MBP/hexahistidine tagged variant of the MS2 coat protein

Depositor

Jeffrey Kieft, Robert Batey

Article

Batey et al RNA. 2007 Aug;13(8):1384-9. Epub 2007 Ju

Insert

Hexahistidine/Maltose binding protein/MS2 coat protein fusion

Use

Tags

Hexahistidine tag

Expression

Bacterial

Mutation

Promoter

T7 RNA polymerase

Available since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-Rab13-T22N

Plasmid

#110501

Purpose

Dominant Negative

Depositor

Bernardo Mainou

Article

Mainou et al J Virol. 2012 Aug;86(16):8346-58. doi: 1

Insert

Rab13-T22N (RAB13 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

T22N

Promoter

Available since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-mNaChBacMut-T2A-tdTomato

Plasmid

#60657

Purpose

Expresses mNaChBacMut-T2A-tdTomato

Depositor

Massimo Scanziani

Article

Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

Insert

mNaChBacMut-T2A-tdTomato

Use

Tags

Expression

Mammalian

Mutation

E191K

Promoter

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pDonor_AcCAST_kanR

Plasmid

#127925

Purpose

Kanamycin resistance donor for AcCAST

Depositor

Feng Zhang

Article

Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

Insert

KanR

Use

Tags

Expression

Mutation

Promoter

Available since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMAL-p2X

Plasmid

#75287

Purpose

Expresses proteins in the periplasm as fusions to maltose-binding protein

Depositor

Paul Riggs

Article

Walker et al Appl Microbiol Biotechnol. 2010 Sep;88(1

Type

Empty backbone

Use

Tags

maltose-binding protein

Expression

Bacterial

Mutation

Promoter

tac

Available since

July 18, 2016

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pPT24-mtnluc

Plasmid

#184339

Purpose

Transformation of mitochondrial DNA in yeast

Depositor

Martin Ott

Article

Rzepka et al STAR Protoc. 2022 May 25;3(2):101359. do

Insert

nanoluciferase recoded for mitochondrial expression flanked by COX2 UTRs

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
miniSOG-GFP

Plasmid

#185580

Purpose

for CALI

Depositor

Mamiko Yajima

Article

Wavreil et al Dev Biol. 2021 Oct;478:13-24. doi: 10.10

Insert

miniSOG

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Expression

Mutation

Promoter

Available since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMAL-c2E

Plasmid

#75291

Purpose

Expresses proteins in the cytoplasm as fusions to maltose-binding protein

Depositor

Paul Riggs

Article

Walker et al Appl Microbiol Biotechnol. 2010 Sep;88(1

Type

Empty backbone

Use

Tags

maltose-binding protein

Expression

Bacterial

Mutation

Promoter

tac

Available since

July 20, 2016

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
humanMER20MER39

Plasmid

#35256

Depositor

Gunter Wagner

Article

Emera et al Proc Natl Acad Sci U S A. 2012 Jul 10;10

Insert

MER20/MER39

Use

Luciferase

Tags

Expression

Mutation

Promoter

dPRL promoter

Available since

March 19, 2012

Availability

Academic Institutions and Nonprofits only
pJOE6261.2

Plasmid

#135079

Purpose

P. putida KT2440 rescue construct

Depositor

Josef Altenbuchner

Article

Graf et al Appl Environ Microbiol. 2011 Aug;77(15):

Insert

upp (upp Pseudomonas putida)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
cmv-sfGFP-GB1-scAB

Plasmid

#185794

Purpose

scFv GFP compnent for SunRISER

Depositor

Robin Lee

Article

Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

Insert

sfGFP-GB1-scAB

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
R5 MCS

Plasmid

#51732

Purpose

Dicistronic vector with cloning site

Depositor

Vincent Mauro

Article

Burman et al Nucleic Acids Res. 2012 Sep;40(16):8085-

Insert

MCS

Use

Tags

Expression

Mutation

Promoter

SV40

Available since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pTU-A-005

Plasmid

#124412

Purpose

Slot A, contains mKO2-MarA (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot A with mKO2-MarA reporter + terminator + spacer

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTfdc1Sc

Plasmid

#78287

Purpose

Expresses S. cerevisiae FDC1 in E. coli

Depositor

David Nielsen

Article

McKenna et al Metab Eng. 2011 Sep;13(5):544-54. doi: 1

Insert

FDC1 (FDC1 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

trc

Available since

June 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pTU-B-005

Plasmid

#124413

Purpose

Slot B, contains sfGFP-MarAn20 (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot B with sfGFP-MarAn20 reporter + terminator + spacer

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTU-C-005

Plasmid

#124414

Purpose

Slot C, contains RepA70-mKate2 (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot C with RepA70-mKate2 reporter + terminator

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTU-B-000

Plasmid

#124410

Purpose

Slot B, empty (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot B + terminator + spacer

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTU-C-000

Plasmid

#124411

Purpose

Slot C, empty (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot C + terminator

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMAL-c2G

Plasmid

#75290

Purpose

Expresses proteins in the cytoplasm as fusions to maltose-binding protein

Depositor

Paul Riggs

Article

Walker et al Appl Microbiol Biotechnol. 2010 Sep;88(1

Type

Empty backbone

Use

Tags

maltose-binding protein

Expression

Bacterial

Mutation

Promoter

tac

Available since

July 20, 2016

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pJK285

Plasmid

#71551

Purpose

Production of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag (H6AaAlr)

Depositor

T. J. Kappock

Article

Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

Insert

alanine racemase

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pHIS-Hsv2

Plasmid

#42524

Depositor

James Hurley

Article

Baskaran et al Mol Cell. 2012 Aug 10;47(3):339-48. doi:

Insert

Hsv2

Use

Tags

HIS-TEV

Expression

Bacterial

Mutation

Promoter

Available since

July 25, 2013

Availability

Academic Institutions and Nonprofits only
Dendra-SpVasa

Plasmid

#185583

Purpose

for photoconversion

Depositor

Mamiko Yajima

Article

Wavreil et al Dev Biol. 2021 Oct;478:13-24. doi: 10.10

Insert

Vssa

Use

In vitro transcription (mrna synthesis)

Tags

Dendra

Expression

Mutation

Promoter

Available since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Kaede-SpVasa

Plasmid

#185582

Purpose

for photoconversion

Depositor

Mamiko Yajima

Article

Wavreil et al Dev Biol. 2021 Oct;478:13-24. doi: 10.10

Insert

Vasa

Use

In vitro transcription (mrna synthesis)

Tags

Kaede

Expression

Mutation

Promoter

Available since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSP64-mCherry

Plasmid

#135597

Purpose

Expression of mCherry mRNA for microinjection into S. purpuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

mCherry

Use

In vitro transcription (mrna synthesis)

Tags

Expression

Mutation

Promoter

Unknown

Available since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pTU-B-013

Plasmid

#124416

Purpose

Slot B, empty (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot B + terminator + spacer

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTU-C-013

Plasmid

#124417

Purpose

Slot C, empty (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot C + terminator

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTU-A-010

Plasmid

#124418

Purpose

Slot A, contains mKO2-MarA (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot A with mKO2-MarA reporter + terminator + spacer

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTU-B-010

Plasmid

#124419

Purpose

Slot B, contains sfGFP-MarAn20 (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot B with sfGFP-MarAn20 reporter + terminator + spacer

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTU-C-010

Plasmid

#124420

Purpose

Slot C, contains RepA70-mKate2 (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot C with RepA70-mKate2 reporter + terminator

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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