Addgene

We narrowed to 16,210 results for: multi

Showing: 3781 - 3800 of 16210 results

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pJFRC150-20XUAS-IVS-Flp1::PEST

Plasmid

#32132

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

Flippase1::PEST

Use

Tags

PEST

Expression

Insect

Mutation

Aspartic Acid at Amino Acid 5

Promoter

Available since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only

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pEE083

Plasmid

#149279

Purpose

T-DNA encoding TRV2 complete genome with MCS

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pmAmetrine1.2

Plasmid

#42171

Depositor

Robert Campbell

Article

Ding et al Anal Chem. 2011 Dec 15;83(24):9687-93. d

Insert

mAmetrine1.2

Use

Tags

6His

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pTPGI_dCas9

Plasmid

#64279

Purpose

encodes yeast-optimized dCas9 synthetic transcription factor

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

Yeast-optimized dCas9

Use

Tags

SV40 NLS

Expression

Yeast

Mutation

D10A & H840A relative to S. pyogenes Cas9

Promoter

pTPGI

Available since

May 5, 2015

Availability

Academic Institutions and Nonprofits only

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pMSCVII-BFP

Plasmid

#162755

Purpose

Retroviral (MSCV) expression of mTagBFP2 fluorescent protein

Depositor

Maki Nakayama

Article

Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

Insert

mTagBFP2

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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438-GST

Plasmid

#97092

Purpose

GST-tev-yORF

Depositor

Chris Jeans

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Use

Tags

GST tag and TEV cleavage site

Expression

Insect

Mutation

Promoter

Polyhedrin promoter

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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pActinin-LSSmOrange

Plasmid

#37139

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 Apr 24.

Insert

LSSmOrange

Use

Tags

human alpha-actinin

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

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p5E-ESyn1

Plasmid

#80804

Purpose

hSyn1 promoter fused to CMVmin

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

p5E-ESyn1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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VV064: 1xCox8 - ratiometric pHluorin in fck

Plasmid

#58502

Purpose

Expresses the pH sensor ratiometric pHluorin in the mitochondria of mammalian cells

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

1xCox8 - Ratiometric pHluorin

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

a-CamKII

Available since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pSUP202pol4

Plasmid

#67184

Purpose

Conjugative cloning vector

Depositor

Hauke Hennecke, Julia Vorholt

Article

Fischer et al EMBO J. 1993 Jul;12(7):2901-12.

Type

Empty backbone

Use

Conjugative bacterial cloning vector

Tags

Expression

Mutation

Promoter

Available since

July 29, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HisFlag-CAD

Plasmid

#31615

Depositor

Aziz Sancar

Article

Lindsey-Boltz et al Nucleic Acids Res. 2004 . 32(15):4524-3

Insert

CAD

Use

Tags

3xFlag, His, and T7

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2012

Availability

Academic Institutions and Nonprofits only
pJMD14

Plasmid

#138654

Purpose

phiOZJ integrating Streptomyces expression vector, intact RDF

Depositor

Joshua Blodgett

Article

Ko et al J Ind Microbiol Biotechnol. 2019 Nov 8.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

PermE* variant sequence from pDA1652

Available since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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pMA-MsgRNA-EGFP

Plasmid

#80794

Purpose

For insertion of gRNA array containing 11-30 gRNA modules

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pAWp30

Plasmid

#73857

Purpose

pFUGW-EFSp-Cas9-P2A-Zeo

Depositor

Timothy Lu

Article

Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

Insert

EFSp-Cas9-P2A-Zeo

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-MBP-yATOM_Ras

Plasmid

#209709

Purpose

Mammalian expression of y-ATOM biosensor for HRas

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

MBP-yATOM_Ras

Use

Tags

MBP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCVII-mChe

Plasmid

#162753

Purpose

Retroviral (MSCV) expression of mCherry fluorescent protein

Depositor

Maki Nakayama

Article

Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

Insert

mCherry

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-Tyr-P(11)4

Plasmid

#185784

Purpose

Encodes enzyme tyrosinase linked to P(11)4 self-assembling peptide via a GS-linker sequence

Depositor

Lizhong He

Article

Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

Insert

Tyr-P(11)4

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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BSP610

Plasmid

#122255

Purpose

expression vector for mtagBFP2 under EFT3 promoter

Depositor

Alexander Mendenhall

Article

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

mtagBFP2

Use

Tags

Expression

Worm

Mutation

Promoter

EFT3

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pME-tdTomato no stop

Plasmid

#82404

Purpose

Gateway-compatible tdTomato fusion reporter

Depositor

Judith Eisen

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

tdTomato (lacks stop codon)

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pUHD-SB-mAID/Zeo

Plasmid

#171681

Purpose

TRE-controlled mAID-fusion sleeping beauty transposon vector with selection marker (zeocin)

Depositor

Randy Poon

Article

Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.

Type

Empty backbone

Use

Tags

mAID

Expression

Mammalian

Mutation

Promoter

TRE

Available since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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