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pET20b-mPTER-D298A

Plasmid

#222191

Purpose

Bacterial expression of mouse PTER-D298A protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

PTER (Pter Mouse)

Use

Tags

6xHis and Strep

Expression

Bacterial

Mutation

D298A

Promoter

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET20b-mPTER-H28F

Plasmid

#222193

Purpose

Bacterial expression of mouse PTER-H28F protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

PTER (Pter Mouse)

Use

Tags

6xHis and Strep

Expression

Bacterial

Mutation

H28F

Promoter

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET20b-mPTER-H201F

Plasmid

#222194

Purpose

Bacterial expression of mouse PTER-H201F protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

PTER (Pter Mouse)

Use

Tags

6xHis and Strep

Expression

Bacterial

Mutation

H201A

Promoter

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET20b-mPTER-R233A

Plasmid

#222197

Purpose

Bacterial expression of mouse PTER-R233A protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

PTER (Pter Mouse)

Use

Tags

6xHis and Strep

Expression

Bacterial

Mutation

R233A

Promoter

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET20b-mPTER-H300A

Plasmid

#222198

Purpose

Bacterial expression of mouse PTER-H300A protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

PTER (Pter Mouse)

Use

Tags

6xHis and Strep

Expression

Bacterial

Mutation

H300A

Promoter

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET20b-mPTER-L255A

Plasmid

#222199

Purpose

Bacterial expression of mouse PTER-L255A protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

PTER (Pter Mouse)

Use

Tags

6xHis and Strep

Expression

Bacterial

Mutation

L255A

Promoter

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET20b-mPTER-L260G

Plasmid

#222200

Purpose

Bacterial expression of mouse PTER-L260G protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

PTER (Pter Mouse)

Use

Tags

6xHis and Strep

Expression

Bacterial

Mutation

L260G

Promoter

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET20b-mPTER-T258G

Plasmid

#222201

Purpose

Bacterial expression of mouse PTER-T258G protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

PTER (Pter Mouse)

Use

Tags

6xHis and Strep

Expression

Bacterial

Mutation

T258G

Promoter

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET20b-mPTER-R304K

Plasmid

#222202

Purpose

Bacterial expression of mouse PTER-R304K protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

PTER (Pter Mouse)

Use

Tags

6xHis and Strep

Expression

Bacterial

Mutation

R304K

Promoter

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET20b-mPTER-Y65F

Plasmid

#222203

Purpose

Bacterial expression of mouse PTER-Y65F protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

PTER (Pter Mouse)

Use

Tags

6xHis and Strep

Expression

Bacterial

Mutation

Y65F

Promoter

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET20b-mPTER-Y263F

Plasmid

#222204

Purpose

Bacterial expression of mouse PTER-Y263F protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

PTER (Pter Mouse)

Use

Tags

6xHis and Strep

Expression

Bacterial

Mutation

Y263F

Promoter

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNK189

Plasmid

#219743

Purpose

MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Homo sapiens

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

mutant of fungal hispidin-3-hydroxylase

Use

Synthetic Biology

Tags

Expression

Mutation

D37E, V181I, S323M, M385K

Promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pNK4169

Plasmid

#219744

Purpose

MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Pichia pastoris

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

mutant of fungal hispidin-3-hydroxylase

Use

Synthetic Biology

Tags

Expression

Mutation

D37E, V181I, S323M, M385K

Promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pNK7183

Plasmid

#219760

Purpose

MoClo-compatible Level 1 for expression PzPKS2 in plants (N.benthamiana, BY2 cells)

Depositor

Karen Sarkisyan

Article

Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

Insert

PzPKS2

Use

Tags

Expression

Plant

Mutation

Promoter

p35s_0.4kb - 5'UTR TMV omega

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pNK7187

Plasmid

#219761

Purpose

MoClo-compatible Level 1 for expression PpASCL in plants (N.benthamiana, BY2 cells)

Depositor

Karen Sarkisyan

Article

Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

Insert

PpASCL

Use

Tags

Expression

Plant

Mutation

Promoter

p35s_0.4kb - 5'UTR TMV omega

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNK7180

Plasmid

#219762

Purpose

MoClo-compatible Level 1 for expression HmS in plants (N.benthamiana, BY2 cells)

Depositor

Karen Sarkisyan

Article

Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

Insert

HmS

Use

Tags

Expression

Plant

Mutation

Promoter

p35s_0.4kb - 5'UTR TMV omega

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-IRES-GFP/CALR ins5 YD/FL-FLAG

Plasmid

#214706

Purpose

Mammalian expression of human CALR ins5 YD/FL-FLAG

Depositor

Norio Komatsu

Article

Masubuchi et al Leukemia. 2020 Feb;34(2):499-509. doi: 1

Insert

CALR ins5 YD/FL-FLAG (CALR Human)

Use

Retroviral

Tags

FLAG

Expression

Mutation

Promoter

MSCV

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-AtSLAC1 8D

Plasmid

#212948

Purpose

Vector used for expression of AtSLAC1 8D in mammalian cell

Depositor

Sangho Lee

Article

Lee et al Nat Commun. 2023 Nov 14;14(1):7345. doi:

Insert

Slow anion channel-associated 1 (OZS1 Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

S59D/T62D/S65D/S86D/S107D/S124D/S146D/S152D

Promoter

CMV

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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METTL3

Plasmid

#155725

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

METTL3 (METTL3 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

H330Q compared to BC052244, no mutations compared…

Promoter

EF-1a

Available since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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EDC3

Plasmid

#155561

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

EDC3 (EDC3 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge