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Plasmid#219745PurposeMoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 codon-optimised for expression in Nicotiana benthamianaDepositorInsertmutant of fungal luciferase
UseLuciferase and Synthetic BiologyTagsExpressionMutationI3S, N4T, F11L, I63T, T99P, T192S, A199PPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK116
Plasmid#219742PurposeMoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Nicotiana benthamianaDepositorInsertmutant of fungal hispidin-3-hydroxylase
UseSynthetic BiologyTagsExpressionMutationD37E, V181I, S323M, M385KPromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX304-PCDH7-c
Plasmid#108540PurposeExpresses human PCDH7 transcript variant c in mammalian cellsDepositorInsertPCDH7 transcript variant c (PCDH7 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLX304-PCDH7-b
Plasmid#108539PurposeExpresses human PCDH7 transcript variant b in mammalian cellsDepositorInsertPCDH7 transcript variant b (PCDH7 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pPPI4-Strep-SNAP-CTLA-4-His6
Plasmid#223583PurposeProtein purification of human CTLA4 with Twin-Strep, SNAP, GCN4 and 6xHis tag from mammalian cellsDepositorInsertCTLA4 (CTLA4 Human)
UseTagsHis6 and Twin-Strep, SNAPExpressionMammalianMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
mouse Oct4-GFP GOF18 transgenic reporter
Plasmid#60527PurposeBAC recombineering generated construct, used as a reporter for Oct4 expression (containes both DE and PE elements)DepositorInsertGFPpA
UseTagsExpressionMammalianMutationPromoterOct4Available sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
eGFP-proα2(I)-L
Plasmid#120160PurposeExpresses mouse Type I procollagen α2 chain (Col1a2) with eGFP replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage siteDepositorInsertType I procollagen α2 chain (Col1a2 Mouse)
UseTagseGFPExpressionMammalianMutationCol1a2 exons 2-3 replaced by fluorescent tagPromoterCMVAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pEF019 MBP-β-catenin-His
Plasmid#154070PurposeExpresses MBP-β-catenin-His (human β-catenin as a fusion protein with MBP and His- tags) in E.coliDepositorInsertCTNNB1 (CTNNB1 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromoterTacAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLAG-puro-PTPIP51(1-150_175-470)
Plasmid#170537PurposeExpresses PTPIP51 FFAT-like motif (151-175) deletion mutant (1-150_176-470) in human HeLa cells after transfectionDepositorInsertProtein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
UseTagsFLAG tagExpressionMammalianMutationdeleted amino acids 151-175PromoterCAG promoterAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLX317-MBNL2
Plasmid#115444PurposeConstitutive lentiviral expressionDepositorInsertMBNL2 (MBNL2 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1REST_sh_seq2_WPRE
Plasmid#127574PurposeshRNA to knock down RE1-silencing transcription factor (REST)DepositorInsertshRNA for knock down of REST (REST Human)
UseLentiviralTagsExpressionMutationPromoterU6Available sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGex-4T-2_LC3A
Plasmid#73946PurposeFor recombinant expression of human LC3A/MAP1LC3A in E. coliDepositorInsertLC3A (MAP1LC3A Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
mApple-proα2(I)-L
Plasmid#120161PurposeExpresses mouse Type I procollagen α2 chain (Col1a2) with mApple replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage siteDepositorInsertType I procollagen α2 chain (Col1a2 Mouse)
UseTagsmAppleExpressionMammalianMutationCol1a2 exons 2-3 replaced by fluorescent tagPromoterCMVAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETM6-4F2-mCherry
Plasmid#73421PurposeReporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4F2.DepositorInsertPromoter 4F2 (orthogonal T7-lac variant)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterP4F2 (orthogonal T7-lac variant)Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
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Flag-hPKL WT
Plasmid#107159PurposeFor mammalian expression of Flag-hPKL WTDepositorInsertFlag-hPKL WT (PKLR Human)
UseRetroviralTagsN-term FlagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-REST_sh_seq1_WPRE
Plasmid#127573PurposeshRNA to knock down RE1-silencing transcription factor (REST)DepositorInsertshRNA for knock down of REST (REST Human)
UseLentiviralTagsExpressionMutationPromoterU6Available sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only