Showing: 3781 - 3800 of 4617 results
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Plasmid#165127Purposemammalian expression and localizationDepositorInsertNsp13 deltaN1 (ORF1ab codon optimized SARS-COV-2)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Nsp13 deltaN2-EGFP
Plasmid#165128Purposemammalian expression and localizationDepositorInsertNsp13 deltaN2 (ORF1ab codon optimized SARS-COV-2)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Nsp13 K289A-EGFP
Plasmid#165130Purposemammalian expression and localizationDepositorInsertNsp13 K289A (ORF1ab codon optimized SARS-COV-2)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
CMV-RfxCas13d-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA
Plasmid#171380PurposeExpression vector for encoding a human codon-optimized RfxCas13d driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.DepositorInserthumanized RfxCas13d
UseTagsHAExpressionMammalianMutationPromoterCMV, U6AvailabilityAcademic Institutions and Nonprofits only -
pBOB-CAG-SARS-CoV2-Spike D614G-HA
Plasmid#158761Purpose3rd Generation lentiviral vector expressing the codon optimized SARS-CoV2 Spike Glycoprotein D614G high infectivity mutant with a C-terminal HA tag generated by Junko Ogawa & Gerald M PaoDepositorInsertSARS-CoV2 Spike Glycoprotein (S SARS-CoV2 hCoV19_USA EPI_ISL_414366 (GISAID))
UseLentiviralTagsHAExpressionMammalianMutationSpike D614GPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
ATX3-13Q
Plasmid#184248PurposeRecombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track fused with His-Tag and TEV cleavage sequence (N-terminal)DepositorInsertataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track (ATXN3 Human, Synthetic)
UseTags6x His-Tag and TEV cleavage siteExpressionBacterialMutationC-terminal codon optimization for protein expres…PromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
HEpl016
Plasmid#179991PurposepCBh_3xFLAG_NLS_SiCas12i_NLS_pA_pCMV_mCherry_pU6_crRNA_BbsI: vector for encoding a human codon-optimized SiCas12i driven by CBh promoter and tagmCherry driven by CMV promoterDepositorInsertSiCas12i
UseTags3xFlagExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
HEpl017
Plasmid#179992PurposepCBh_NLS_TadA8e_dSiCas12i_NLS_pA_pCMV_mCherry_pU6_crRNA_BbsI: vector for encoding a human codon-optimized dSiCas12i-ABE8e driven by CBh promoter and tagmCherry driven by CMV promoterDepositorInsertTadA8e-dSiCas12i(D1049A)
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCAT079
Plasmid#180509PurposePlasmid expressing mammalian codon optimized wt DpbCasX, sgRNAv2 scaffold, and restriction sites to clone in new spacersDepositorInsertsCasX sgRNAv2
DpbCasX
UseCRISPRTags2x FLAG and SV40 NLSExpressionMammalianMutationPromoterCAG and U6AvailabilityAcademic Institutions and Nonprofits only -
pCAT105
Plasmid#180510PurposePlasmid expressing mammalian codon optimized engineered DpbCasX-R3, sgRNAv2 scaffold, and restriction sites to clone in new spacersDepositorInsertsCasX sgRNAv2
DpbCasX with R3 loop
UseCRISPRTags2x FLAG and SV40 NLSExpressionMammalianMutationPromoterCAG and U6AvailabilityAcademic Institutions and Nonprofits only -
pCAT077
Plasmid#180511PurposePlasmid expressing mammalian codon optimized wt PlmCasX, sgRNAv2 scaffold, and restriction sites to clone in new spacersDepositorInsertsCasX sgRNAv2
PlmCasX
UseCRISPRTags2x FLAG and SV40 NLSExpressionMammalianMutationPromoterCAG and U6AvailabilityAcademic Institutions and Nonprofits only -
pCAT100
Plasmid#180512PurposePlasmid expressing mammalian codon optimized engineered chimeric PlmCasX-R1, sgRNAv2 scaffold, and restriction sites to clone in new spacersDepositorInsertsCasX sgRNAv2
PlmCasX with DpbCasX R1 loop
UseCRISPRTags2x FLAG and SV40 NLSExpressionMammalianMutationPromoterCAG and U6AvailabilityAcademic Institutions and Nonprofits only -
pCAT526
Plasmid#180513PurposePlasmid expressing mammalian codon optimized wt PlmCasX, mNeonGreen, sgRNAv1 scaffold, and restriction sites to clone in new spacersDepositorInsertsCasX sgRNAv1
PlmCasX-2A-mNeonGreen
UseCRISPRTags2x FLAG and SV40 NLSExpressionMammalianMutationPromoterCAG and U6AvailabilityAcademic Institutions and Nonprofits only -
pCAT527
Plasmid#180514PurposePlasmid expressing mammalian codon optimized engineered chimeric PlmCasX-R1, mNeonGreen, sgRNAv2 scaffold, and restriction sites to clone in new spacersDepositorInsertsCasX sgRNAv2
PlmCasX with DpbCasX R1 loop-2A-mNeonGreen
UseCRISPRTags2x FLAG and SV40 NLSExpressionMammalianMutationPromoterCAG and U6AvailabilityAcademic Institutions and Nonprofits only -
pJJGL004
Plasmid#180608PurposePlasmid expressing E. coli codon optimized His-MBP-TEV-PlmCasX+Region1 insertionDepositorInsertPlmCasX with DpbCasX R1 loop
UseCRISPRTags10xHis and MBPExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET28-SpRY-HF1-NLS-6xHis (gbr2102)
Plasmid#181744Purposebacterial expression plasmid for SpRY-HF1 with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tagDepositorInsertbacterial codon optimized SpRY-HF1
UseIn vitro transcription; t7 promoterTagsBPNLS(SV40)-6xHis and MKIEE MBP tagExpressionBacterialMutationSpRY-HF1, SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E…PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET-PE2-His (IK1822)
Plasmid#170103PurposeT7 promoter bacterial expression plasmid for PE2 (nSpCas9(H840A)-MMLV-RT) with C-terminal 6xHis-tag and bipartite NLSDepositorInsertbpNLS-nSpCas9(H840A)-MMLVRT-bpNLS-6xHis
UseCRISPRTags6xHis tagExpressionBacterialMutationBacteria codon-optimized nSpCas9 (H840A) & M-…PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
FUW-crRNA1and2-U6_TRE-dLbCpf1-NLS-hCTCF_K365A_R368A-HA-P2A-tagBFP
Plasmid#194886PurposeDox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-K365A_R368A mutant and crRNA-1 and -2 targeting the human MECP2 locus.DepositorInsertsdLbCpf1-NLS-hCTCF_K365A_R368A
U6-crRNA1and2
UseLentiviralTagsHAExpressionMammalianMutationHuman codon optimized catalytically inactive LbCp…PromoterTRE and U6AvailabilityAcademic Institutions and Nonprofits only -
FUW-crRNA1and2-U6_TRE-dLbCpf1-NLS-hCTCF_Q418A-HA-P2A-tagBFP
Plasmid#194887PurposeDox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-Q418A mutant and crRNA-1 and -2 targeting the human MECP2 locus.DepositorInsertsdLbCpf1-NLS-hCTCF_Q418A
U6-crRNA1and2
UseLentiviralTagsHAExpressionMammalianMutationHuman codon optimized catalytically inactive LbCp…PromoterTRE and U6AvailabilityAcademic Institutions and Nonprofits only -
FUW-crRNA1and2-U6_TRE-dLbCpf1-NLS-hCTCF_R396A-HA-P2A-tagBFP
Plasmid#194888PurposeDox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-R396A mutant and crRNA-1 and -2 targeting the human MECP2 locus.DepositorInsertsdLbCpf1-NLS-hCTCF_R396A
U6-crRNA1and2
UseLentiviralTagsHAExpressionMammalianMutationHuman codon optimized catalytically inactive LbCp…PromoterTRE and U6AvailabilityAcademic Institutions and Nonprofits only
Showing: 3781 - 3800 of 4617 results