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Showing: 3781 - 3800 of 30473 results

  7. pAd/CMV/DAX1-IRES-nEGFP

    Plasmid
    #29752
    Depositor
    Edward McCabe
    Insert
    dosage-sensitive sex reversal, adrenal hypoplasia critical region, on chromosome X, gene 1 (NR0B1 Human)
    Use
    Tags
    EGFP, IRES, and Nuclear localization sequence
    Expression
    Mammalian
    Mutation
    No mutations; Reporter is nuclear localized EGFP;…
    Promoter
    Available since
    Oct. 21, 2011
    Availability
    Academic Institutions and Nonprofits only
    29752-plasmid-map-sequence-id-213749

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  8. pDEST-CMV GFP-LC3B G120A

    Plasmid
    #123115
    Purpose
    Expresses EGFP-LC3B G120A in mammalian cells. Negative control fluorescent reporter, unable to localize to autophagosomes.
    Depositor
    Robin Ketteler
    Article
    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155
    Insert
    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Glycine 120 to Alanine
    Promoter
    CMV
    Available since
    May 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    123115-plasmid-map-sequence-id-244026

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  9. pCMV-T7-SpRY-P2A-mScarlet (MNW002)

    Plasmid
    #174137
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R); C-terminal bi-partite NLS, 3x flag tag, P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpRY with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    SpG=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317…
    Promoter
    CMV and T7
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174137-plasmid-map-sequence-id-341916

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  10. CMV-tdTomato-P2A-paCaMKII(T286A)

    Plasmid
    #165432
    Purpose
    Expresses tdTomato and photoactivatable CaMKII(T286A: Autophosphorylation deficient) in mammalian cells to induce synaptic plasticity.
    Depositor
    Hideji Murakoshi
    Article
    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1
    Insert
    tdTomato-P2A-paCaMKII(T286A)
    Use
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    T286A: Autophosphorylation deficient mutation
    Promoter
    CMV
    Available since
    March 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    165432-plasmid-map-sequence-id-325984

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  17. pTetON-DHFRdd-SpvB; CMV-mCherry

    Plasmid
    #89463
    Purpose
    Tet/Dox-inducible expression of DHRFdd-SpvB (Salmonella SpvB 375-591) plus constitutive expression of mCherry in mammalian cells (Dual-promoter)
    Depositor
    Brad Zuchero
    Article
    Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n
    Insert
    DeAct-SpvB (spvB Salmonella enterica)
    Use
    Tags
    E. coli DHFRdd destabilization domain and mCherry…
    Expression
    Mammalian
    Mutation
    Truncation spanning SpvB amino acids 375-591 (mon…
    Promoter
    TRE3G
    Available since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    89463-plasmid-map-sequence-id-176808

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  19. pAAV-CMV-FLEX-SaCas9-U6-sgHtr2a

    Plasmid
    #159908
    Purpose
    Mutagenesis of Htr2a
    Depositor
    Larry Zweifel
    Insert
    Htr2a (Htr2a Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159908-plasmid-map-sequence-id-317314

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Showing: 3781 - 3800 of 30473 results