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Showing: 9451 - 9500 of 156554 results

  3. G protein alpha-q-GFP

    Plasmid
    #66080
    Purpose
    This G protein alpha-q construct contains internal insertions of GFP and the EE epitope
    Depositor
    Catherine Berlot
    Article
    Hughes et al J Biol Chem. 2001 Feb 9;276(6):4227-35.
    Insert
    G protein alpha-q-EE-YFP (Gnaq Mouse)
    Use
    Tags
    GFP was inserted internally into the alpha-q sequ…
    Expression
    Mammalian
    Mutation
    BamHI and SacI sites in alpha-q were removed with…
    Promoter
    CMV
    Available since
    Aug. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    66080-plasmid-map-sequence-id-436984

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  4. Gs(short)-CASE

    Plasmid
    #168124
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gs. Composed of the subunits G alpha s(short isoform) (GNAS) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 1 (GNG1).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Insert
    GNAS, GNB1, GNG1 (GNGT1, GNAS, GNB1 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG1
    Expression
    Mammalian
    Mutation
    NLuc is inserted at N136/V137 within GNAS
    Promoter
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168124-plasmid-map-sequence-id-332868

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  7. pXPR_071

    Plasmid
    #164558
    Purpose
    Higher titer version of pXPR_053. Lentiviral vector that enables constitutive sgRNA expression. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.
    Depositor
    Arlene Sharpe
    Article
    LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s
    Insert
    sgRNA cassette
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human U6
    Available since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    164558-plasmid-map-sequence-id-330194

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  9. pMVS142_pACT1_mCherry_Zif268_EBD_MCS_KAN

    Plasmid
    #99049
    Purpose
    Transcription Factor chassis for activation domains. mCherry, Zif268 DNA binding domain, estrogen response domain
    Depositor
    Barak Cohen
    Article
    Staller et al Cell Syst. 2018 Mar 1. pii: S2405-4712(1
    Inserts
    ACT1 promoter (ACT1 Budding Yeast)
    mCherry
    Zif268 DNA binding Domain
    Estrogen response domain (ESR1 Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    99049-plasmid-map-sequence-id-350965

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  10. pGAL-PDE1

    Plasmid
    #160388
    Purpose
    Overxpression of PDE1 in yeast
    Depositor
    John McCusker
    Insert
    PDE1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    160388-plasmid-map-sequence-id-314523

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  11. mCherry-TGNP-N-10

    Plasmid
    #55145
    Purpose
    Localization: Golgi Complex, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    TGNP (TGOLN2 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55145-plasmid-map-sequence-id-339930

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  26. pQCXIP-Laforin Myc

    Plasmid
    #84656
    Purpose
    mammalian expression and/or retrovirus preparation
    Depositor
    Ayano Satoh
    Article
    Toyota et al Sciencematters
    Insert
    Laforin (EPM2A Human)
    Use
    Retroviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    5'LTR
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only

  28. Flag-APEX3-C Cloning Vector

    Plasmid
    #220615
    Purpose
    To express a protein of interest fused to the C-terminus of Flag-APEX3 for proximity labeling or TEM (unpublished)
    Depositor
    Ken-Ichi Takemaru
    Type
    Empty backbone
    Use
    Tags
    Flag-APEX3 fusion
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    220615-plasmid-map-sequence-id-435941

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  31. pSNAPf-ADRβ2 Control Plasmid

    Plasmid
    #101123
    Purpose
    Expression of SNAP-Tagged β2 Adrenergic Receptor in Mammalian cells
    Depositor
    New England Biolabs, Ana Egana
    Insert
    Beta-2-Adrenergic Receptor (ADRB2 Human)
    Use
    Tags
    SNAP-tag (SNAPf)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 27, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101123-plasmid-map-sequence-id-194092

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  34. ACBE

    Plasmid
    #204607
    Purpose
    Mammalian expression of adenine-to-cytosine base editor ACBE
    Depositor
    Dali Li
    Article
    Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103
    Insert
    bpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e-linker-C terminal of nSpCas9-bpNLS-P2A-GFP
    Use
    CRISPR
    Tags
    6*His
    Expression
    Mammalian
    Mutation
    mAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)
    Promoter
    Available since
    Aug. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    204607-plasmid-map-sequence-id-404719

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  36. pCD5-D/bovine/Nebraska/9-5/2012-HEFs57aED-GCN4-sfGFP-ST

    Plasmid
    #175020
    Purpose
    Expresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFP
    Depositor
    Robert de Vries
    Article
    Nemanichvili et al Viruses. 2021 Mar 31;13(4). pii: v130405
    Insert
    D/bovine/Nebraska/9-5/2012
    Use
    Tags
    GCN4-TEV-sfGFP-TwinStrep
    Expression
    Mammalian
    Mutation
    s57a esterase knock out mutant
    Promoter
    CMV
    Available since
    Jan. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    175020-plasmid-map-sequence-id-350538

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  40. pExp-GB1

    Plasmid
    #112565
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and GB1 fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8xHis-GB1 and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112565-plasmid-map-sequence-id-217228

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  41. PTGR1

    Plasmid
    #38874
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTGR1 (PTGR1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  45. pJKW0474

    Plasmid
    #107588
    Purpose
    pCLV3:Cas9
    Depositor
    Jing-Ke Weng
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Arabidopsis CLAVATA3
    Available since
    April 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    107588-plasmid-map-sequence-id-208235

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  46. pCMV-SHP2 WT

    Plasmid
    #8381
    Depositor
    Ben Neel
    Insert
    SHP2 (PTPN11 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 15, 2005
    Availability
    Academic Institutions and Nonprofits only
    8381-plasmid-map-sequence-id-363016

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  49. His 3C MKK3 1-347

    Plasmid
    #87878
    Purpose
    E. coli expression of MKK3 aa 1-347
    Depositor
    Peter Mace
    Insert
    MKK3 (MAP2K3 Human)
    Use
    Tags
    3C protease site and 6xHis
    Expression
    Bacterial
    Mutation
    aa 1-347
    Promoter
    T7
    Available since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87878-plasmid-map-sequence-id-174923

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Showing: 9451 - 9500 of 156554 results