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Plasmid#69103PurposeExpresses tagRFP (codon adapted for Streptococcus pneumoniae); under control of Zinc inducible promoter PZn. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.DepositorInserttagRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE-3
Plasmid#72678PurposeExpresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Kan resistance marker.DepositorInsertmutL E32K
UseTagsnoneExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…PromoterAvailable sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMK344 (Hygro-P2A-mAID)
Plasmid#121178PurposeN-terminal taggingDepositorInsertHygro-P2A-mAID
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
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BB1_12_YlpGPD
Plasmid#117820PurposePromoter pGPD of Y. lipolytica for GoldenMOCSDepositorInsertYlpGPD
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1a-NICD1
Plasmid#46047DepositorInsertNotch1 (C-term) (Notch1 Mouse)
UseTagsMyc tagExpressionMammalianMutationCytoplasmic domain initiating at amino acid 1753PromoterEF1aAvailable sinceJuly 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
TFORF0689
Plasmid#141662PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertHNF4G (HNF4G Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFV2-HA
Plasmid#187082PurposeExpression of ATP synthase, with all Cys changed to Ala, and subunit a-HA tagged, subunit beta-His tagged)DepositorInsertatpBEFHAGDC
UseTags6-His beta subunit (atpD) and HA tag subunit a (a…ExpressionBacterialMutationAll Cys codons changed to Ala.PromoterAvailable sinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-CD9-10
Plasmid#54029PurposeLocalization: Tetraspanin/Membrane, Excitation: 487, Emission: 509DepositorInsertCD9 (CD9 Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJuly 2, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKK-TEV-CyOFP1
Plasmid#105799PurposeExpression of your protein of interest in fusion with orange fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 27240196). CyOFP1 is useful for multi-channel imaging.DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-CyOFP1ExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
Munc18cwt-pDEST27
Plasmid#85626PurposeGST tagged Munc18cDepositorInsertMunc18c (Stxbp3 Mouse)
UseTagsGSTExpressionMammalianMutationPromoterCMVAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
elavl3:SomaGCaMP7f
Plasmid#158760PurposeCell body-targeted GCaMP7f, under HuC promoter: GCaMP7f followed by a linker, and the EE-RR coiled coil motif (GCaMP7f-27-EE-RR). As bright as conventional GCaMP7f.DepositorInsertSomaGCaMP7
UseSynthetic BiologyTagsExpressionMutationPromoterHuC promoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-dV-IgG1/κ
Plasmid#52213PurposeHuman immunoglobulin gamma 1 antibody expression vector (kappa light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) coloniesDepositorInsertsHuman immunoglobulin gamma 1 constant region
Human immunoglobulin kappa constant region
UseTagsExpressionMammalianMutationDeleted Variable regionPromoterMouse Elongation Factor 1 Alpha and Rat Elongatio…Available sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
TFORF1478
Plasmid#143122PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTP63 (TP63 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF3237
Plasmid#144713PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSOX10 (SOX10 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0693
Plasmid#141663PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertHNF4A (HNF4A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLAG-LOV*-H2B
Plasmid#202204PurposeExpresses the photoproximity labeling catalyst LOV* fused to H2B in mammalian cellsDepositorInsertHistone H2B type 1-K
UseTagsFLAG and LOV*ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-RLuc8
Plasmid#87121PurposeMammalian expression vector expressing mutant vesrion of the reporter gene-Renilla Luciferase (Rluc8), which is more stable in mouse serum and has more light output.DepositorInsertRenilla Luciferase
UseLuciferaseTagsExpressionMammalianMutationA55T, C124A, S130A, K136R, A143M, M185V, M253L, a…PromoterCMVAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMK462 (Hygro-P2A-mAB)
Plasmid#214388PurposemAID-BromoTag for N-terminal taggingDepositorInsertHygro-P2A-mAID-BromoTag
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
IVT-donor_opt(R2Tg)-GFP
Plasmid#223246PurposeIn vitro transcription of the optimized R2Tg RNA donorDepositorInsertR2Tg donor_opt
UseIn vitro transcriptionTagsExpressionMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
IVT-R2Tg_opt
Plasmid#223247PurposeIn vitro transcription of the optimized R2Tg proteinDepositorInsertR2Tg protein_opt
UseIn vitro transcriptionTagsExpressionMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-MAVS(IRSp53 pLxIS)
Plasmid#221315PurposeExpression of MAVS chimera with IRSp53 pLxIS domain in mammalian cells by retroviral transductionDepositorInsertMAVS chimera with IRSp53 pLxIS domain (Mavs Mouse)
UseRetroviralTagsFLAGExpressionMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FKBP-STING(pLxIS)
Plasmid#221240PurposeExpression of STING CTT in mammalian cells by retroviral transductionDepositorInsertSTING CTT (Sting1 Mouse)
UseRetroviralTags3xFLAG-4xFKBPExpressionMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-QUAS:GCaMP7a
Plasmid#184817PurposeUsed to generate the Tg(QUAS:GCaMP7a)c594 transgenic line. GCaMP7a from Muto et al., 2013 (https://doi.org/10.1016/j.cub.2012.12.040).DepositorArticleInsertQUAS:GCaMP7a-pA
UseZebrafish tol2 transgenesisTagsExpressionMutationPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
UAS:TVA-mCherry
Plasmid#187823PurposeExpression of a fusion of the transmembrane protein TVA and the red fluorescent protein mCherry under UAS control.DepositorInsertTVA-mCherry
UseTagsExpressionBacterialMutationPromoter5xUASAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
HCTR1-DuET
Plasmid#213310PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertHCTR1 (HCRTR1 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
Anti-VAPA/B [N479/107R]
Plasmid#188163PurposeMammalian Expression Plasmid of anti-VAPA and -VAPB (Rat). Derived from hybridoma N479/107.DepositorInsertUseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJS124
Plasmid#207593PurposeGFP-BIB H20A expressionDepositorInsertGFP-BIB H20A
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDWJ27
Plasmid#207604PurposeGFP-BIB expressionDepositorInsertGFP-BIB
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
VAPA/B scFv [N479/107]
Plasmid#206742PurposeMammalian Expression of VAPA/B scFV. Derived from hybridoma N479/107 scFv.DepositorInsertVAPA/B (Rattus norvegicus) recombinant scFV (Vapa Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2753
Plasmid#144229PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZNF445 (ZNF445 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-TetOne-Puro-p62 deltaUBA
Plasmid#204550PurposeExpresses p62 deltaUBA in a doxycycline-dependent manner in mammalian cellsDepositorInsertp62 (SQSTM1 Human)
UseRetroviralTagsExpressionMammalianMutationdeltaUBAPromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEntry_HCoV-229E-nsp13
Plasmid#168970PurposeGateway-compatible Entry vectorDepositorInsertHCoV-229E-nsp13 (HCoV229Egp1 )
UseGateway-compatible entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEntry_SARS-CoV-2-nsp13
Plasmid#168890PurposeGateway-compatible Entry vectorDepositorInsertSARS-CoV-2-nsp13 (ORF1ab )
UseGateway-compatible entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2201
Plasmid#143177PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertPOU4F2 (POU4F2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJR019
Plasmid#198834PurposeInactivation of G protein subunits through Pertussis toxin (PTX)DepositorInsert15xUAS::PTX-SL2-GFP::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P-1-eIF4E K119A
Plasmid#199440PurposeBacterial expression plasmids for production of recombinant GST-eIF4E K119ADepositorInsert1977 (EIF4E Human)
UseTagsGST-fusion proteinExpressionBacterialMutationchanged lysine 119 to alaninePromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-KLF6
Plasmid#192909PurposeBarcoded piggybac transposon vector with Dox-inducible expression of KLF6DepositorInsertKLF6 (KLF6 Human)
UseTagsHAExpressionMammalianMutationPromoterTET3G-miniCMVAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pMCherry F8-2
Plasmid#186034PurposeExpress VHH F8-2 as a mCherry fusion in mammalian cellsDepositorInsertVHH F8-2
UseTagsmCherryExpressionMammalianMutationPromoterCMV immediate earlyAvailable sinceJuly 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_SARS-COV-2_ORF10
Plasmid#169398PurposeCell-free/mammalian expression vector for SARS-COV-2 virus ORF10 with N-term eGFPDepositorInsertSARS-COV-2 _ORF10 (ORF10 SARS-Cov2)
UseCell-free expression vectorTagsHis and eGFPExpressionMutationPromoterT7- CMV/CAGAvailable sinceJuly 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
mmu-miR-30a-5p Block-iT design (BLK)
Plasmid#107789PurposemiR-30a RNAi ExpressionDepositorInsertMmu-miR-30a-5p mature sequence (Mir30a Mouse)
UseRNAiTagsEmGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET MBP mRuby2 LIC cloning vector (MBP-mRuby2)
Plasmid#86933PurposepET LIC cloning vector for Biotin-His6-MBP-N10-tev-yORF-mRuby2DepositorTypeEmpty backboneUseTagsBiotin, His6, MBP, Tev Recognition Sequence, and …ExpressionBacterialMutationPromoterT7Available sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEZ01
Plasmid#81124PurposepACSA_Pcpt_gfpDepositorInsertGFP
UseTagsExpressionMutationPromoterPcptAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23 C3_7
Plasmid#60293PurposeThis plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.DepositorInsertSLC30A8 enhancer (SLC30A8 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23 C3_13
Plasmid#60298PurposeThis plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.DepositorInsertEDN3 enhancer (EDN3 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSR657
Plasmid#65214PurposeBaculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-2 cap gene in Sf9 insect cellsDepositorInsertsmodified AAV-2 rep78
modified AAV-2 cap
UseTagsExpressionInsectMutationPromoterp10 and polyhedrinAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
TFORF3205
Plasmid#144681PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertFOXO3 (FOXO3 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-rtTA-IRES-Hygro
Plasmid#102423PurposeMammalian expression vector for tetracycline-transactivator (rtTA), IRES-HygroDepositorInserttetracyclin-transactivator (rtTA)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCoPURO
Plasmid#17533DepositorInsertPuromycine Acetyl Transferase
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only