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Plasmid#74336PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Gap43. Not for gene expression.DepositorInsertgrowth associated protein 43 (Gap43 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceMay 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag/M87 S245X
Plasmid#92368Purposeexpression of M87 S245X spastin in mammalian cellsDepositorInsertspastin (SPAST Human)
UseTagsExpressionMammalianMutation1-460 bp deleted, S245XPromoterCMVAvailable sinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag 3B/WT myc-M87
Plasmid#87722PurposeExpresses WT myc-M87 spastin isoform in mammalian cellsDepositorInsertSPAST (SPAST Human)
UseTagsMycExpressionMammalianMutationM87 isoform (deletion of nt 1-490 - 5'UTR an…PromoterCMVAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRE/SP N184X with 5'UTR
Plasmid#92375PurposeInducible expression of N184X spastin M1 and M87 in mammalian cellsDepositorInsertspastin (SPAST Human)
UseTagsExpressionMammalianMutationS184XPromoterTet-responsive P tightAvailable sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-THTAP-SPARTIN, residues 1-101
Plasmid#180626PurposeBacterial expression for SPARTIN MIT residues 1-101. Has N-terminal HIS-GST tag. Internal ID: WISP20-57.DepositorInsertSPARTIN
UseTagsHIS-GSTExpressionBacterialMutationResidues 1-101PromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-THTAP-CALPAIN-7 1-165
Plasmid#180610PurposeBacterial expression vector for CALPAIN-7 residues 1-165. Has N-terminal cleavable HIS-GST tag.Internal ID: WISP21-129DepositorInsertCALPAIN-7
UseTagsHIS-GSTExpressionBacterialMutationResidues 1-65PromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CALPAIN-7 residues 1-165
Plasmid#180611PurposeBacterial expression vector for CALPAIN-7 residues 1-165, tandem MIT domains. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-25DepositorInsertCALPAIN-7
UseTagsHIS-SUMOExpressionBacterialMutationResidues 1-165PromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-Rtn2b-R60f-mCherry
Plasmid#186607PurposeThird generation lentiviral vector expressing Reticulon 2 disease mutant R60f fused to mCherryDepositorInsertHuman Reticulon 2 mutant R60f (RTN2 Human)
UseLentiviralTagsmCherryExpressionMammalianMutationc.178insC (R60f)PromoterCMVAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
Rtn2b-NTD136-271-mNeon
Plasmid#186604PurposeEncodes human Reticulon 2 isoform B N-terminal domain aa 136 to 271 fused to mNeonGreenDepositorInsertHuman Reticulon 2 isoform B amino acids 136 to 271 (RTN2 Human)
UseTagsmNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRE/SP M87 N184X
Plasmid#92373PurposeInducible expression of M87 N184X spastin in mammalian cellsDepositorInsertspastin (SPAST Human)
UseTagsExpressionMammalianMutation1-460 bp deleted, N184XPromoterTet-responsive P tightAvailable sinceJune 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRE/SP S245X with 5'UTR
Plasmid#92376PurposeInducible expression of S245X spastin M1 and M87 in mammalian cellsDepositorInsertspastin (SPAST Human)
UseTagsExpressionMammalianMutationS245XPromoterTet-responsive P tightAvailable sinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRE/SP M87 S245X
Plasmid#92374PurposeInducible expression of M87 S245X spastin in mammalian cellsDepositorInsertspastin (SPAST Human)
UseTagsExpressionMammalianMutation1-460 bp deleted, S245XPromoterTet-responsive P tightAvailable sinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRE/SP M1 S245X
Plasmid#92372PurposeInducible expression of M1S245X spastin in mammalian cellsDepositorInsertspastin (SPAST Human)
UseTagsExpressionMammalianMutation1-194 bp of 5'UTR deleted, S245XPromoterTet-responsive P tightAvailable sinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRE/SP M1 N184X
Plasmid#92371PurposeInducible expression of M1 N184X spastin in mammalian cellsDepositorInsertspastin (SPAST Human)
UseTagsExpressionMammalianMutation1-194 bp of 5'UTR deleted, N184XPromoterTet-responsive P tightAvailable sinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag/SP S245X with 5'UTR
Plasmid#92370Purposeexpression of 245X spastin M1 and M87 in mammalian cellsDepositorInsertspastin (SPAST Human)
UseTagsExpressionMammalianMutationS245XPromoterCMVAvailable sinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag/M87 N184X
Plasmid#92367Purposeexpression of M87 N184X spastin in mammalian cellsDepositorInsertspastin (SPAST Human)
UseTagsExpressionMammalianMutation1-460 bp deleted, N184XPromoterCMVAvailable sinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag/M1 N184X
Plasmid#92365Purposeexpression of M1 N184X spastin in mammalian cellsDepositorInsertspastin (SPAST Human)
UseTagsExpressionMammalianMutation1-194 bp of 5'UTR deleted, N184XPromoterCMVAvailable sinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag 3B/myc-M87L195V
Plasmid#87721PurposeExpresses myc-M87L195V spastin isoform in mammalian cellsDepositorInsertSPAST (SPAST Human)
UseTagsMycExpressionMammalianMutationM87 isoform (deletion of nt 1-490 - 5'UTR an…PromoterCMVAvailable sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag/S46X without 5'UTR
Plasmid#87730PurposeExpresses S46X spastin M1 and M87 in mammalian cellsDepositorInsertSPAST (SPAST Human)
UseTagsExpressionMammalianMutationS46X; 5'UTR deleted (1-190 bp)PromoterCMVAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag/S44L without 5'UTR
Plasmid#87728PurposeExpresses S44L spastin M1 and M87 in mammalian cellsDepositorInsertSPAST (SPAST Human)
UseTagsExpressionMammalianMutationS44L; 5'UTR deleted (1-190 bp)PromoterCMVAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only