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Plasmid#8453Purpose3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses puromycin for selection.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2005AvailabilityAcademic Institutions and Nonprofits only
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PB-gRNA-Puro
Plasmid#121121PurposeExpression of a gRNA together with a Puromycin resistanceDepositorInsertgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSCV Puro-CCSP:GFP
Plasmid#68487PurposeRetrovirus expressing GFP driven by CCSP promoterDepositorInsertsPuromycin Resistance
GFP
UseRetroviralTagsExpressionMammalianMutationPromoterCCSP and LTRAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P23
Plasmid#120034PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with HaloDepositorInsertHalo
UsePcr templateTagsHaloExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P34
Plasmid#120030PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 3xFLAGDepositorInsert3xFLAG
UsePcr templateTags3xFLAGExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P05
Plasmid#120016PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with eGFPDepositorInserteGFP
UsePcr templateTagseGFPExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P28
Plasmid#120039PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 3xHADepositorInsert3xHA
UsePcr templateTags3xHAExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P22
Plasmid#120033PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with SNAPDepositorInsertSNAP
UsePcr templateTagsSNAPExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P33
Plasmid#120018PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with FLAGDepositorInsertFLAG
UsePcr templateTagsFLAGExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P27
Plasmid#120038PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 1xHADepositorInsert1xHA
UsePcr templateTags1xHAExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-tdTomato
Plasmid#159275PurposeHuman AAVS1 targeting vector for knockin of a CAGGS promoter-driven tdTomato cassette (cloned between AgeI and PacI). Targeted cells will be puromycin resistant.DepositorInserttdTomato
UseAAV; S1 knockin donor vectorTagsExpressionMammalianMutationPromoterCAGGSAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-ERP2
Plasmid#80478PurposeAll-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry and constitutive rtTA and puromycin resistance)DepositorTypeEmpty backboneUsePiggybac transposonTagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR_Puro_sgTP53
Plasmid#187819PurposepLentiCRISPR that is selectable with puromycin with an sgRNA targeting TP53DepositorInsertsgTP53
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-CERP2
Plasmid#175499PurposeAll-in-one piggyBac transposon vector for mCMV+dox-inducible expression of Golden Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G-mCMVAvailable sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pscALPS- SIV-MAC251 vpx
Plasmid#115810PurposeEncodes codon optimized 3xFLAG-VpxMAC251 and puromycin resistance proteinDepositorInsertSIVMAC251 Vpx
UseLentiviralTags3x-FLAGExpressionMutationPromoterSFFVAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-T3G-CERP2
Plasmid#175498PurposeAll-in-one piggyBac transposon vector for dox-inducible expression of Golden Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-CERP2-MG
Plasmid#175503PurposeAll-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G-mCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-TAG-ERP2
Plasmid#80479PurposeAll-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry and constitutive rtTA and puromycin resistance)DepositorTypeEmpty backboneUsePiggybac transposonTagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
PL552
Plasmid#68407PurposeDonor empty vector with floxed puromycin resistance gene expression cassetteDepositorTypeEmpty backboneUseCre/LoxTagsExpressionMammalianMutationPromotermouse PGK promoterAvailable sinceNov. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Puro
Plasmid#111886PurposeLentiviral expression of cDNA with puromycin resistance geneDepositorTypeEmpty backboneUseLentiviralTagsFLAGExpressionMammalianMutationPromoterEFS promoterAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only