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Showing: 361 - 367 of 367 results

  1. GAP50-COMP-blac-flag-his

    Plasmid
    #110956
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    secreted acid phosphatase (GAP50) (PF3D7_0918000 Synthetic)
    Use
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Sept. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    110956-plasmid-map-sequence-id-221989

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  2. PIESP15-COMP-blac-flag-his

    Plasmid
    #110958
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    parasite-infected erythrocyte surface protein (PIESP15) (PF3D7_0103900 Synthetic)
    Use
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Sept. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    110958-plasmid-map-sequence-id-223542

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  3. pU6-Universal

    Plasmid
    #52694
    Purpose
    This plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.
    Depositor
    Sebastian Lourido
    Inserts
    Cas9
    Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
    Use
    CRISPR; Toxoplasma gondii
    Tags
    3X FLAG and Nuclear localization signal
    Expression
    Mutation
    Promoter
    TgTUB1 and Toxoplasma U6
    Available since
    June 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    52694-plasmid-map-sequence-id-82051

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  5. pcDNA-GFP-BAEBL (I1134V/P1135L/Y1136V/F1137V)

    Plasmid
    #154875
    Purpose
    Expresses N-terminally GFP-tagged Pf-BAEBL P5-P2 VLVV variant (coding for residues 1041-1210) from pcDNA3.1
    Depositor
    Sinisa Urban
    Article
    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-
    Insert
    PfBAEBL (PF3D7_0506900 P. falciparum)
    Use
    Tags
    GFP and signal peptide (residues 1 to 32) from TG…
    Expression
    Mammalian
    Mutation
    I1134V, P1135L, Y1136V, F1137V; insert codes for …
    Promoter
    CMV
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    154875-plasmid-map-sequence-id-310429

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Showing: 361 - 367 of 367 results