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Plasmid#160804PurposeExpress C-terminal ISC mutant POLE1DepositorInsertPOLE1 C-ISCmut (POLE Human)
UseRetroviralTags3xFLAGExpressionMutationshPOLE_1 and shPOLE2 resistant, C2221S, C2224S, C…PromoterAvailable sinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSPL3-ABCC8-27
Plasmid#135919PurposeEncodes ABCC8 exon 27 wild type for the analysis of splicing variantsDepositorInsertATP-binding Cassette subfamily C member 8 (ABCC8 Human)
UseExon trappingTagsExpressionMammalianMutationPromoterSV40Available sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast POLD1 ISCmut
Plasmid#160806PurposeExpress ISC mutant POLD1DepositorInsertPOLD1 ISCmut (POLD1 Human)
UseRetroviralTagsExpressionMutationCodon optimized C-terminal 230 amino acids, C1058…PromoterAvailable sinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast POLA1 ISCmut
Plasmid#160808PurposeExpress ISC mutant POLA1DepositorInsertPOLA1 ISCmut (POLA1 Human)
UseRetroviralTagsExpressionMutationCodon optimized, C1354S, C1359S, C1377S, C1380SPromoterAvailable sinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTripZ EV
Plasmid#209071PurposeDOX-ON inducible expression vector that has a stuffer sequence after an LR reaction and used as empty expression vector controlDepositorInsertstuffer DNA sequence after LR recombination with pTripZ NEO DEST to be used as an empty vector control
UseLentiviralTags3XFLAGExpressionMammalianMutationPromoterCMVAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTripz NEO DEST
Plasmid#209072PurposeModified DOX-ON inducible destination vector with a c-terminal 3X flag tag to be used for LR recombination reactions to inducibly express cDNAs of interestDepositorTypeEmpty backboneUseLentiviral; Gateway destination vector to be used…Tags3X FLAGExpressionMutationPromoterCMVAvailable sinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
R703-M72-303: CMV51p> Halotag-Hs.KRAS4b
Plasmid#159679PurposeMammalian protein expression of KRAS4b-Halotag fusionDepositorInsertHalotag-Hs.KRAS4b (KRAS Human)
UseTagsHalotagExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
R713-M88-303: CMV51p> Halotag-Hs.HRAS
Plasmid#159681PurposeMammalian protein expression of HRAS-Halotag fusionDepositorInsertHalotag-Hs.HRAS (HRAS Human)
UseTagsHalotagExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
R713-M85-303: CMV51p> Halotag-Hs.KRAS4a
Plasmid#159680PurposeMammalian protein expression of KRAS4a-Halotag fusionDepositorInsertHalotag-Hs.KRAS4a (KRAS Human)
UseTagsHalotagExpressionMammalianMutationPromoterAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
HisGB1-CITED2(216-269)+CBPTAZ1(340-439)
Plasmid#173761PurposeBacterial coexpression of human CITED2 CTAD with CBP TAZ1DepositorUseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTripZ RB1 WT-3XFLAG
Plasmid#209070PurposeDOX-ON inducible expression vector that expresses RB1 WT with c-terminal 3X flag tagDepositorInserthuman RB1 WT after LR recombination with pTripZ NEO DEST to inducibly express RB1 WT with a c-terminal 3X flag tag
UseLentiviralTags3XFLAGExpressionMammalianMutationPromoterCMVAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
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psfTn5
Plasmid#79107Purposebacterial expression of hyperactive Tn5 transposase carrying E54K and L372P mutationsDepositorInserttransposase 5
UseTagsHisExpressionBacterialMutationE54K and L372PPromoterAvailable sinceMarch 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-45b(+)-Tn5
Plasmid#112112PurposeExpresses hyperactive Tn5 transposase for next generation sequencing library preparationDepositorInsertTn5
UseTagsHis6ExpressionBacterialMutationE54K E110K P242A L372PPromoterAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
SypHer
Plasmid#48250PurposepH sensorDepositorInsertSypHer
UseTagsExpressionMammalianMutationC199SPromoterCMVAvailable sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOSV00170
Plasmid#222934PurposeConstitutive GFP expression for Bacillus subtilisDepositorInsertGFP(Sp)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPhyperspankAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
SypHer mt
Plasmid#48251PurposepH sensor, targeted to mitochondriaDepositorInsertSypHer
UseTags2 mitochondrial targeting sequencesExpressionMammalianMutationC199SPromoterCMVAvailable sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
VR122 pETv2 10His-pG-Tn5 (E54K, L372P)
Plasmid#198467PurposeE. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in DH5α cloning strain.DepositorInsert10His-pG-Tn5 (E54K, L372P)
UseTags10His-pGExpressionBacterialMutationE54K, L372PPromoterConsensus φ10 T7 PromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pA-His6-Tn5
Plasmid#171871PurposeExpresses protein A and Tn5 Transposase fusion protein in bacterial cellsDepositorInsertProtein A and hyperactive Tn5 transposase
UseTagsHis-tagExpressionBacterialMutationWTPromoterAvailable sinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDV17_pCMV-MCP-ADAR2dd(E488Q)-NES
Plasmid#200406PurposeExpresses a compact, engineered version of ADAR2 constitutively in mammalian cellsDepositorInsertMCP-ADAR2dd(E488Q)-NES
UseSynthetic BiologyTagsExpressionMammalianMutationE488Q (hyperactive)PromoterCMVAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only