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CRISPRi-CENPB-g3

Plasmid

#120211

Purpose

CENPB CRISPRi guide RNA 3

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

CENPB CRISPRi guide RNA

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 8, 2020

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2-Snrnp40

Plasmid

#134255

Purpose

Lentivector for Snrnp40 CRISPR knockout

Depositor

Bruce Beutler

Article

Zhang et al Nat Immunol. 2019 Oct;20(10):1322-1334.

Insert

Snrp40 (Snrnp40 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Snrnp40 gRNA “GATAACTATGCGACGTTGAA”

Promoter

U6

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv1-CENPB

Plasmid

#120208

Purpose

gRNA targeting the stem loop of CENPB poly(A) site

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

CENPB poly(A) site

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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CRISPRi-CENPB-g9

Plasmid

#120217

Purpose

CENPB CRISPRi guide RNA 9

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

CENPB CRISPRi guide RNA

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
CRISPRi-CENPB-g8

Plasmid

#120216

Purpose

CENPB CRISPRi guide RNA 8

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

CENPB CRISPRi guide RNA

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
CRISPRi-CENPB-g7

Plasmid

#120215

Purpose

CENPB CRISPRi guide RNA 7

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

CENPB CRISPRi guide RNA

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
CRISPRi-CENPB-g6

Plasmid

#120214

Purpose

CENPB CRISPRi guide RNA 6

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

CENPB CRISPRi guide RNA

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
CRISPRi-CENPB-g5

Plasmid

#120213

Purpose

CENPB CRISPRi guide RNA 5

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

CENPB CRISPRi guide RNA

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
CRISPRi-CENPB-g2

Plasmid

#120210

Purpose

CENPB CRISPRi guide RNA 2

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

CENPB CRISPRi guide RNA

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
CRISPRi-CENPB-g1

Plasmid

#120209

Purpose

CENPB CRISPRi guide RNA 1

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

CENPB CRISPRi guide RNA

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223 SCAF8_isoD_CRISPR_resistant

Plasmid

#122468

Purpose

pDONR for Gateway cloning

Depositor

Jesper Svejstrup

Article

Gregersen et al Cell. 2019 May 10. pii: S0092-8674(19)30

Insert

SCAF8 isoform D CRISPR resistant (SCAF8 Human)

Use

Gateway cloning

Tags

Expression

Mutation

Synonymous mutations conferring resistance to gRN…

Promoter

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pFS_0376_pET30-RT-CRISPR_Tolypothrix_campylonemoides_ARRAY_1_RC

Plasmid

#116988

Purpose

Expresses TcRT-Cas1-Cas2 under pT7lac promoter, encodes TcCRISPR array 1 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Tolypothrix campylonemoides RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0357_pET30-RT-CRISPR_Micromonospora_rosaria_ARRAY_1

Plasmid

#116969

Purpose

Expresses MrRT-Cas1-Cas2 under pT7lac promoter, encodes MrCRISPR array 1, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Micromonospora rosaria RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0358_pET30-RT-CRISPR_Micromonospora_rosaria_ARRAY_1_RC

Plasmid

#116970

Purpose

Expresses MrRT-Cas1-Cas2 under pT7lac promoter, encodes MrCRISPR array 1 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Micromonospora rosaria RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0369_pET30-RT-CRISPR_Azospirillum_lipoferum_4B_ARRAY_1

Plasmid

#116981

Purpose

Expresses AlRT-Cas1-Cas2 under pT7lac promoter, encodes AlCRISPR array 1, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Azospirillum lipoferum 4B RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0380_pET30-RT-CRISPR_Tolypothrix_campylonemoides_ARRAY_3_RC

Plasmid

#116992

Purpose

Expresses TcRT-Cas1-Cas2 under pT7lac promoter, encodes TcCRISPR array 3 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Tolypothrix campylonemoides RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0359_pET30-RT-CRISPR_Micromonospora_rosaria_ARRAY_2

Plasmid

#116971

Purpose

Expresses MrRT-Cas1-Cas2 under pT7lac promoter, encodes MrCRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Micromonospora rosaria RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0366_pET30-RT-CRISPR_Desulfarculus_baarsii_DSM_2075_ARRAY_2_RC

Plasmid

#116978

Purpose

Expresses DbRT-Cas1-Cas2 under pT7lac promoter, encodes DbCRISPR array 2 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Desulfarculus baarsii DSM 2075 RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0367_pET30-RT-CRISPR_Woodsholea_maritima_ARRAY_1

Plasmid

#116979

Purpose

Expresses WmRT-Cas1-Cas2 under pT7lac promoter, encodes WmCRISPR array 1, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Woodsholea maritima RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0368_pET30-RT-CRISPR_Woodsholea_maritima_ARRAY_1_RC

Plasmid

#116980

Purpose

Expresses WmRT-Cas1-Cas2 under pT7lac promoter, encodes WmCRISPR array 1 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Woodsholea maritima RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge