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pRRL-mouse STING #2-gRNA-Cas9-Puro

Plasmid

#186893

Purpose

gRNA targeting mouse STING (with Cas9 insert)

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Cas9 (Sting1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-STING L373A with IRSp53 pLxIS

Plasmid

#221283

Purpose

Expression of STING L373A with IRSp53 pLxIS (RLL361NLV) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

fusion of FKBP-STING (L373A) with IRSp53 pLxIS (RLL361NLV) (Sting1, Baiap2 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Expression

Mutation

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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p931-SWITCH-OVER insert: EF1a-Blasti

Plasmid

#217890

Purpose

insert vector for Switch-OVER: Single loxP-STOP-EF1a-Blasti-mU6

Depositor

Ulrich Elling

Article

Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCA528 SPASTIN , F124D, residues 108-200

Plasmid

#180629

Purpose

Bacterial expression for SPASTIN MIT, residues 108-200. Has F124D mutation. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-12.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

SPASTIN

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 108-200, F124D

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 SPASTIN , L177D, residues 108-200

Plasmid

#180634

Purpose

Bacterial expression for SPASTIN MIT, residues 108-200. Has L177D mutation. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-13.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

SPASTIN

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 108-200, L177D

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-FLAG-IRES-Blasticidin-hOCT4 S236D

Plasmid

#198765

Purpose

expression of hOCT4 mutants

Depositor

Hyonchol Jang

Article

Kim et al Cancers (Basel). 2020 Sep 11;12(9):2601.

Insert

hOCT4 236D (POU5F1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S236D

Promoter

Available since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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pPB UbC Arrestin-uTEV1-V5; UAS-mCherry

Plasmid

#173122

Purpose

Expresses uTEV1 and reporter receiver construct in DIPG

Depositor

Alice Ting

Article

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

Arrestin-uTEV1-V5; UAS-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

UbC

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pPB UbC Arrestin-TEVp(WT)-V5; UAS-mCherry

Plasmid

#173121

Purpose

Expresses TEVp(WT) and reporter receiver construct in DIPG

Depositor

Alice Ting

Article

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

Arrestin-TEVp(WT)-V5; UAS-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

UbC

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pSEM264 - pDESTR4-R3 mosTI Pmlc-2_GFP target

Plasmid

#159842

Purpose

Single copy or array insertion by MosTI using split Pmlc-2 GFP selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

pDESTR4-R3 mosTI Pmlc-2_GFP target

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pSEM322 - array co-injection fragment mosTI neoR

Plasmid

#159832

Purpose

Single copy or array insertion by MosTI using split NeoR selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

array co-injection fragment mosTI neoR

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pFastBac Dual PNG172-STII PLU-HIS

Plasmid

#112992

Purpose

Baculovirus expression of Drosophila png172-Flag and plu-His

Depositor

Terry Orr-Weaver

Inserts

png172 (png Fly)
plu (plu Fly)

Use

Tags

Flag and His

Expression

Insect

Mutation

png172 mutation

Promoter

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pFastBac Dual PNG-STII PLU-HIS

Plasmid

#112993

Purpose

Baculovirus expression of Drosophila png-Flag and plu-His

Depositor

Terry Orr-Weaver

Inserts

png (png Fly)
plu (plu Fly)

Use

Tags

Flag and His

Expression

Insect

Mutation

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pSLPB2B-FKBP12_F36V-hHDAC4-SpdCas9-tagBFP-PGK-Blasticidin

Plasmid

#187956

Purpose

FKBP12 (F36V mutant) degron-tagged hHDAC4-dCas9 fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

Depositor

Edda Schulz

Article

Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

Insert

FKBP12_F36V-hHDAC4-SpdCas9-tagBFP

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pSH-EF1a-OsTIR1.F74A-mCherry-IRES-puro

Plasmid

#216247

Purpose

Express OsTIR1.F74A mutant with mCherry tag for AID2

Depositor

Elina Ikonen

Article

Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

Insert

OsTIR1.F74A

Use

CRISPR and TALEN; Human safe harbor locus (a avs1…

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pSLPB2B-FKBP12_F36V-SpdCas9-KRAB-tagBFP-PGK-Blasticidin

Plasmid

#187953

Purpose

FKBP12 (F36V mutant) degron-tagged dCas9-KRAB domain fused to tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

Depositor

Edda Schulz

Article

Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

Insert

FKBP12_F36V-SpdCas9-KRAB-tagBFP

Use

CRISPR and Synthetic Biology

Tags

GGS

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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MTK Lentivirus Multi Cassette Destination Vector (pAN2424)

Plasmid

#194221

Purpose

Backbone for constructing multi casette expression plasmids using the Mammalian Tookit

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pSEM388 - mosTI unc-119 - NNN - mScarlet - tbb-2

Plasmid

#182348

Purpose

mScarlet fusion cloning vector for mosTI(unc-119). Transgene inserted upstream of mScarlet and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp

Plasmid

#125228

Purpose

Expresses SPARK2 NanoLuc-βarrestin2-TEVp in mammalian cells

Depositor

Alice Ting

Article

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp

Use

AAV

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pSEM320 - sgRNA mosTI unc-119 array insertion

Plasmid

#159824

Purpose

Single copy or array insertion by MosTI using split unc-119 selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

sgRNA mosTI unc-119 array insertion

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSEM394 - mosTI unc-119 - PUPN - NNN - tbb-2

Plasmid

#182352

Purpose

Pan-neuronal expression cloning vector for mosTI(unc-119). Transgene inserted between Ultra pan neuronal (UPN) promoter and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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