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Plasmid#78129Purposeluciferase reporter for miRNA activityDepositorInsertHOXD13 (HOXD13 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutation3'UTR containing miRNA binding sitesPromoterPGKAvailable sinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-sgRNA_HOXD13-mcherry
Plasmid#159793PurposeS. pyogenes sgRNA collocated with pegRNA targeting mouse HOXD13 geneDepositorInsertspacer of sgRNA targeting HOXD13 gene (HOXD13 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJR017
Plasmid#198835PurposeIn vivo blocking of synaptic signaling through Tetanus toxin mediated cleavage of synptobrevin proteins.DepositorInsert15xUAS::TeTX-SL2-mKate::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJR032
Plasmid#198773PurposeStabilized step function Opsins ChR2 variantDepositorInsert15xUAS::hChR2 (C128S, D156A)-EYFP::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJR035
Plasmid#198821PurposeFor tissue specific RNA-seqDepositorInsert15xUAS::rpl-22HA-SL2-mKate::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHW491
Plasmid#198808PurposeIn vivo blocking of synaptic signaling through Tetanus toxin mediated cleavage of synptobrevin proteins.DepositorInsert15xUAS::Tetx cDNA::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJL049
Plasmid#198809PurposeIn vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity.DepositorInsert15xUAS::GCaMP6s-SL2-mKate2::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJR031
Plasmid#198825PurposeStable step function ChR2 variant. Activated by bluelight, terminated by green light.DepositorInsert15xUAS::ChR2(C128S)-EYFP::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJR021
Plasmid#198814PurposeVariant of yellow fluorescent protein for transient gene expressionDepositorInsert15xUAS::destabilizedYFP::let-838 3'UTR
UseTagsExpressionWormMutationYFP with destabilized signal from mouse ornithine…PromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJR034
Plasmid#198829PurposeStep-Waveform Inhibitory ChannelrhodopsinDepositorInsert15xUAS::SwiCHR-TS-EYFP::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJR033
Plasmid#198822PurposeChloride-conducting channelrhodopsinDepositorInsert15xUAS::UAS-iC1-c2-TS-EYFP::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHW449
Plasmid#198805PurposeDirect light-gated cation channel from Chlamydomonas reinhardtii that allows neuron deploarization through brief pulses of blue light.DepositorInsert15xUAS::hChR2(H134R)-YFP::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2531
Plasmid#142954PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSOX9 (SOX9 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
Mito-mTurq2-Apollo-NADP+
Plasmid#193303PurposeExpresses mTurq2-Apollo-NADP+ sensor localized to the mitochondriaDepositorInsertG6PD (G6PD Human)
UseTagsmTurqoise2 and mitochondrial targeting sequenceExpressionMammalianMutationS40A, R72Q, H201N, K205TPromoterT7Available sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
tetO-SOX9
Plasmid#170703Purposedoxycycline-inducible overexpression of SOX9DepositorInsertSOX9 (SOX9 Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cyto-mTurq2-Apollo-NADP+
Plasmid#193301PurposeExpresses mTurq2-Apollo-NADP+ sensor localized to the cytoplasmDepositorInsertG6PD (G6PD Human)
UseTagsmTurqoise2ExpressionMammalianMutationS40A, R72Q, H201N, K205TPromoterT7Available sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHW581
Plasmid#198818PurposeIn vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity. Based on GCaMP7, improved SNR, fast kineticsDepositorInsert15xUAS::GCaMP7f-SL2-mKate2::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
SOX9-T2A-H2B-EGFP repair template
Plasmid#167972PurposeSOX9 reporter repair templateDepositorInsertSRY-Box Transcription Factor 9 (SOX9 Human)
UseCRISPR; Donor templateTagsH2B-EGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJL046
Plasmid#198807PurposeIn vivo neuronal inhibition through histamine-gated chloride channel. Neurons expressing HisCl1 transgene are inhibited after addition of histamine.DepositorInsert15xUAS::HisCl1-SL2-GFP::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJR014
Plasmid#198813PurposeNatural light-gated anion channels. In vivo inhibition of neurons using blue light.DepositorInsert15xUAS::GtACR2::SL2::GFP::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only