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Showing: 361 - 380 of 1114 results

  2. pET-30a(+)-Syk-tSH2-PM

    Plasmid
    #111271
    Purpose
    express murine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation, in E.coli strain Rosetta 2 (DE3)
    Depositor
    Carol Post
    Article
    Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5
    Insert
    murine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation (Syk Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y130E mutation to mimic phosphorylation
    Promoter
    T7 promoter
    Available since
    July 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111271-plasmid-map-sequence-id-215610

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  6. pIC291

    Plasmid
    #51403
    Purpose
    Retroviral expression of EGFP-TEV-S-tag SKA1 for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Article
    Welburn et al Dev Cell. 2009 Mar;16(3):374-85. doi: 10
    Insert
    Ska1 IMAGE 3909951 (SKA1 Human)
    Use
    Retroviral
    Tags
    EGFP, S tag, and TEV protease site
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2014
    Availability
    Academic Institutions and Nonprofits only
    51403-plasmid-map-sequence-id-78323

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  12. pIRTetR-GAL80

    Plasmid
    #185853
    Purpose
    Plugging the mechanism of tetracycline-mediated repression of GAL promoters in Saccharomyces cerevisiae
    Depositor
    Claudia Vickers
    Article
    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:
    Insert
    gal80Arm1-PAgTEF1-KanMX4-TAgTEF1-PHAC1>TetR>TUP1>PABF1- PTEF1+[TetO]>GAL80Arm2
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    185853-plasmid-map-sequence-id-370762

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  13. pILGFP3A2 (2)

    Plasmid
    #185852
    Purpose
    Testing the TEF1 promoter appended with 3 tetracycline riboswitch using a green fluorescence protein as the reporter
    Depositor
    Claudia Vickers
    Article
    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:
    Insert
    PURA3>KlURA3>TAgTEF1-PTEF1>3*TcRb>yEGFP>TPGK1-TURA3
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    185852-plasmid-map-sequence-id-370761

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Showing: 361 - 380 of 1114 results