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Plasmid#221730PurposeExpression of zebrafish Ago2 with N-terminal 3xFLAG tag in mammalian cells.DepositorInsertdrAgo2 (ago2 Zebrafish)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-hsAgo2
Plasmid#221718PurposeExpression of human Ago2 in zebrafish embryos.DepositorInserthsAgo2 (AGO2 Human)
UseFish expressionTagsExpressionMutationPromotersimian CMV IE94Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-drAgo2
Plasmid#221712PurposeExpression of zebrafish Ago2 with N-terminal 3xFLAG tag in zebrafish embryos.DepositorInsertdrAgo2 (ago2 Zebrafish)
UseFish expressionTags3xFLAGExpressionMutationPromoterAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3-FLAG3-drAgo2DY-EF
Plasmid#221733PurposeExpression of zebrafish Ago2 with D651E and Y680F mutations and N-terminal 3xFLAG tag in mammalian cells.DepositorInsertdrAgo2DY-EF (ago2 Zebrafish)
UseTags3xFLAGExpressionMammalianMutationD651E and Y680F in active sitePromoterCMVAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-hsAgo2F-Y
Plasmid#221722PurposeExpression of human Ago2 with F666Y mutation and N-terminal 3xFLAG tag in zebrafish embryos.DepositorInserthsAgo2F-Y (AGO2 Human)
UseFish expressionTags3xFLAGExpressionMutationF666Y in active sitePromotersimian CMV IE94Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-hsAgo2E-D
Plasmid#221721PurposeExpression of human Ago2 with E637D mutation and N-terminal 3xFLAG tag in zebrafish embryos.DepositorInserthsAgo2E-D (AGO2 Human)
UseFish expressionTags3xFLAGExpressionMutationE637D in active sitePromotersimian CMV IE94Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-hsAgo2D-A
Plasmid#221720PurposeExpression of human Ago2 with D669A mutation and N-terminal 3xFLAG tag in zebrafish embryos.DepositorInserthsAgo2D-A (AGO2 Human)
UseFish expressionTags3xFLAGExpressionMutationD669A in active sitePromotersimian CMV IE94Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-hsAgo2
Plasmid#221719PurposeExpression of human Ago2 with N-terminal 3xFLAG tag in zebrafish embryos.DepositorInserthsAgo2 (AGO2 Human)
UseFish expressionTags3xFLAGExpressionMutationPromotersimian CMV IE94Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-drAgo2DY-EF
Plasmid#221716PurposeExpression of zebrafish Ago2 with D651E and Y680F mutations and N-terminal 3xFLAG tag in zebrafish embryos.DepositorInsertdrAgo2DY-EF (ago2 Zebrafish)
UseFish expressionTags3xFLAGExpressionMutationD651E and Y680F in active sitePromotersimian CMV IE94Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-drAgo2Y-F
Plasmid#221715PurposeExpression of zebrafish Ago2 with Y680F mutation and N-terminal 3xFLAG tag in zebrafish embryos.DepositorInsertdrAgo2Y-F (ago2 Zebrafish)
UseFish expressionTags3xFLAGExpressionMutationY680F in active sitePromotersimian CMV IE94Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-drAgo2D-E
Plasmid#221714PurposeExpression of zebrafish Ago2 with D651E mutation and N-terminal 3xFLAG tag in zebrafish embryos.DepositorInsertdrAgo2D-E (ago2 Zebrafish)
UseFish expressionTags3xFLAGExpressionMutationD651E in active sitePromotersimian CMV IE94Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-hsAgo2EF-DY
Plasmid#221723PurposeExpression of human Ago2 with E637D and F666Y mutations and N-terminal 3xFLAG tag in zebrafish embryos.DepositorInserthsAgo2EF-DY (AGO2 Human)
UseFish expressionTags3xFLAGExpressionMutationE637D and F666Y in active sitePromotersimian CMV IE94Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-DmAgo1_572-984-optim_K
Plasmid#146728PurposeBacterial Expression of DmAgo1_572-984-optimDepositorInsertDmAgo1_572-984-optim (AGO1 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28c(plus)-DmAGO1-RC_F
Plasmid#146262PurposeBacterial Expression of DmAGO1-RCDepositorInsertDmAGO1-RC (AGO1 Fly)
UseTagsExpressionBacterialMutationone non silent mutation R787C compared to sequenc…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO2-RB-del55-967_B
Plasmid#145931PurposeInsect Expression of DmAGO2-RB-del55-967DepositorInsertDmAGO2-RB-del55-967 (AGO2 Fly)
UseTagsExpressionInsectMutationIsoform B of DmAGO2 with a deletion of AA 55-967 …PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBluescript-mouse eIF2C3
Plasmid#50355PurposeMouse eIF2C3 cDNA cloneDepositorInserteIF2C3 (Ago3 Mouse)
UseTagsExpressionBacterialMutationPromoterT3Available sinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBluescript-mouse eIF2C4
Plasmid#50356PurposeMouse eIF2C4 cDNA cloneDepositorInserteIF2C4 (Ago4 Mouse)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-I716AR771AF777A-siRNAres_G
Plasmid#146421PurposeInsect Expression of DmAGO1-I716AR771AF777A-siRNAresDepositorInsertDmAGO1-I716AR771AF777A-siRNAres (AGO1 Fly)
UseTagsExpressionInsectMutationvariation with 2 non silent mutations V315A and H…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-I716AR771A-siRNAres_G
Plasmid#146422PurposeInsect Expression of DmAGO1-I716AR771A-siRNAresDepositorInsertDmAGO1-I716AR771A-siRNAres (AGO1 Fly)
UseTagsExpressionInsectMutationvariation with 2 non silent mutations V315A and H…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only