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Plasmid#119140PurposeBase editor made from APOBEC3H Haplotype IDepositorInsertAPOBEC3H Haplotype I-Cas9 nickase-UGI
UseCRISPRTagsExpressionMutationNonePromoterCMVAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf226
Plasmid#12914PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf226
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 29, 2006AvailabilityAcademic Institutions and Nonprofits only -
pX330_NPM2_cterm4
Plasmid#222914PurposeCas9/sgRNA plasmid for targeting NPM2DepositorInsertCas9, NPM2 sgRNA 4 (NPM2 Synthetic, Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMV DJ-1 C106A
Plasmid#220914PurposeExpresses DJ-1 with the C106A mutationDepositorInsertDJ-1 (PARK7 Human)
UseTagsExpressionMammalianMutationC106APromoterCMVAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD044
Plasmid#212914PurposeEncodes the S. cerevisiae 142 amino acid SCW4 Translational Fusion Partner Sequence as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertSCW4 (SCW4 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
p5E-pth2
Plasmid#191428PurposeTol2 Gateway 5' entry vector containing 2067 bp upstream of the CDS for zebrafish pth2 (ENSDARG00000022951), including the 5' UTR and first intron (GRCz11 chr 17:33493999-33496066)DepositorInsertpth2 regulatory region (pth2 Zebrafish)
UseTol2 gateway 5' entry vectorTagsExpressionMutationPromoterAvailable sinceFeb. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-miniSOG-C-Far
Plasmid#193914PurposeControl plasmid that expresses miniSOG targeted to the cell membrane in mammalian cellsDepositorInsertminiSOG
UseAAVTagsFarnesylation signal sequence and Flag tagExpressionMammalianMutationPromoterCAGAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJH4140
Plasmid#191479PurposePacr-2s-loxP::EBFP::loxP::Chrimson::wCherry unc-54 3' UTR C.elegans A/B MN expression of Arch RFPDepositorInsertChrimson
UseTagswCherryExpressionWormMutationPromoterPacr-2sAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH3557
Plasmid#191444PurposePttr-39-Chrimson::wCherry unc-54 3' UTR C.elegans D motor neurou expression of Chrimson RFPDepositorInsertChrimson
UseTagswCherryExpressionWormMutationPromoterPttr-39Available sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH3650
Plasmid#191446PurposePacr-2s-Chrimson::wCherry unc-54 3' UTR C.elegans A/B MN expression of Chrimson RFPDepositorInsertChrimson
UseTagswCherryExpressionWormMutationPromoterPacr-2sAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28 bleach.3
Plasmid#191914PurposeEncodes a GFP design, bleach.3DepositorInsertbleach.3
UseTagsHis TagExpressionBacterialMutationS72A V112I Y145M T167V H181I V224IPromoterT7Available sinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX459-sg-mGreenLantern332
Plasmid#179914PurposeAll in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting mGreenLantern fluorescent protein site 332.DepositorInsertmGreenLantern sgRNA
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianMutationPromoterU6Available sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLX208-SAE1
Plasmid#171012PurposeEctopic expression of SAE1DepositorInsertuntagged SAE1
UseLentiviralTagsExpressionMammalianMutationPromoterEf1-alphaAvailable sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLX208-nfBFP
Plasmid#171013PurposeEctopic expression of nonfluorescent BFPDepositorInsertuntagged nfBFP
UseLentiviralTagsExpressionMammalianMutationBFP[H66A]PromoterEf1-alphaAvailable sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSG5-MAGE-TA360
Plasmid#89141Purposemammalian expression of full-length human MAGE-A11 amino acid residues 2-429 with TA360 phosphorylation site mutatedDepositorInsertMAGE-A11 (MAGEA11 Human)
UseTagsExpressionMammalianMutationT360APromoterSV40Available sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
Ac 1.8kb red opsin DsRed
Plasmid#72914Purposered opsin promoter from Carolina anole lizard (Anolis carolinensis) gDNA (nucleotides21,797 to 187, corresponding to chr2:88663108–88664997 in anoCar2.0) driving DsRedDepositorInsertred opsin promoter
UseTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR-JNKKTRmRuby2
Plasmid#59148PurposeORF encoding JNK KTR mRuby2 flanked by Gateway sequencesDepositorInsertJNK Kinase Translocation Reporter (MAPK8 Human, Mouse)
UseTagsmRuby2ExpressionMutationPromoterAvailable sinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR-p38KTRmCerulean3
Plasmid#59149PurposeORF encoding p38 KTR mCerulean3 flanked by Gateway sequencesDepositorInsertp38 Kinase Translocation Reporter (MAPK14 Human, Mouse)
UseTagsExpressionMutationPromoterAvailable sinceSept. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
N1-Tq-Ca-FLITS
Plasmid#191454PurposeGenetically encoded calcium sensor (GECI) Tq-Ca-FLITS that reports with lifetime and intensity changesDepositorInsertTq-Ca-FLITS
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
retro-gRNA-mRFP1
Plasmid#112914PurposeRetrovirus expressing mRFP1 with BBSI cloning sites for sgRNADepositorInsertmRFP1
UseRetroviralTagsExpressionMutationPromoterEF1aAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only