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MSCV-6NBC-GFP-PGK-Puro-Barcode85

Plasmid

#83762

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode85

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-6NBC-GFP-PGK-Puro-Barcode86

Plasmid

#83763

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode86

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-6NBC-GFP-PGK-Puro-Barcode87

Plasmid

#83764

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode87

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-6NBC-GFP-PGK-Puro-Barcode88

Plasmid

#83765

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode88

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-6NBC-GFP-PGK-Puro-Barcode89

Plasmid

#83766

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode89

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-6NBC-GFP-PGK-Puro-Barcode91

Plasmid

#83768

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode91

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pFusX3-31

Plasmid

#63376

Purpose

Encodes RVD positions 7-9 in TALEN assembly

Depositor

Stephen Ekker

Article

Ma et al Hum Gene Ther. 2016 Jun;27(6):451-63. do

Insert

HD NG NN

Use

TALEN

Tags

Expression

Mutation

none

Promoter

Available since

March 31, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3-Cyto-GCaMP3

Plasmid

#64853

Purpose

Cytosol-targeted version of the Ca2+ indicator GCaMP3.

Depositor

Jin Zhang

Article

Mehta et al Elife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

GCaMP3

Use

Tags

Nuclear export signal (NES)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only

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COM-epegRNA-Dnmt1

Plasmid

#211376

Purpose

Dnmt1 targeting epegRNA with COM insertion in gRNA scaffold for v3b PE-eVLP production

Depositor

David Liu

Article

An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

Insert

COM-epegRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-TetOn-NLS-mCherry

Plasmid

#193762

Purpose

Doxycycline-inducible (TetOn) mCherry with SV40 NLS, control construct

Depositor

Tobias Meyer

Article

Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

Insert

NLS-mCherry

Use

Lentiviral

Tags

mCherry

Expression

Mutation

SV40 NLS fused to mCherry

Promoter

TRE

Available since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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TCARZ-CMV-GFP-PAmin-GF3-R4A22

Plasmid

#203768

Purpose

For production of template RNA for use in PRINT, GFP under CMV promoter

Depositor

Kathleen Collins

Article

Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

Insert

PRINT template RNA for GFP

Use

Ivt template

Tags

Expression

Mutation

Promoter

CMV

Available since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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TCARZ-CBh-GFP-SV40PA-GF3-R4A22

Plasmid

#203767

Purpose

For production of template RNA for use in PRINT, GFP under CBh promoter

Depositor

Kathleen Collins

Article

Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

Insert

PRINT template RNA for GFP

Use

Ivt template

Tags

Expression

Mutation

Promoter

CBh

Available since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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TCARZ-SV40-GFP-PAmin-GF3-R4A22

Plasmid

#203769

Purpose

For production of template RNA for use in PRINT, GFP under SV40 promoter

Depositor

Kathleen Collins

Article

Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

Insert

PRINT template RNA for GFP

Use

Ivt template

Tags

Expression

Mutation

Promoter

SV40

Available since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Cyto-CaNAR2

Plasmid

#64729

Purpose

Cytosol-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.

Depositor

Jin Zhang

Article

Mehta et al Elife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

CaNAR2-NES

Use

Tags

Cerulean3, Nuclear export signal (NES), and YPet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ER-GCaMP3

Plasmid

#64854

Purpose

Endoplasmic reticulum (ER)-targeted version of the Ca2+ indicator GCaMP3; targeted to cytosolic face of ER membrane.

Depositor

Jin Zhang

Article

Mehta et al Elife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

CYP450-GCaMP3

Use

Tags

N-terminal 27 amino acids from CYP450

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_ALPPL2 synNotch

Plasmid

#188376

Purpose

Lentiviral vector - constitutive expression of an anti-ALPPL2 synNotch with a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiALPPL2_synNotch_Gal4VP64

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pTM-BPL

Plasmid

#173766

Purpose

Fluorescent labeling of the nuclear envelope

Depositor

Shinji Sueda

Article

Taniyama et al ACS Chem Biol. 2018 Jun 15;13(6):1463-14

Insert

PDGF receptor TM domain, Biotin protein ligase

Use

Tags

FLAG and Signal peptide

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-Rheb

Plasmid

#133767

Purpose

Expresses GFP-tagged Rheb in mammalian cells

Depositor

Shawn Ferguson

Article

Angarola et al Mol Biol Cell. 2019 Oct 15;30(22):2750-2

Insert

Rheb (RHEB Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 26, 2019

Availability

Academic Institutions and Nonprofits only

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6598-HisSUMO1GG

Plasmid

#13376

Depositor

Anne Dejean

Article

Seeler et al Mol Cell Biol. 2001 May . 21(10):3314-24

Insert

SUMO1 (SUMO1 Human)

Use

T7 in vitro translation

Tags

His

Expression

Mammalian

Mutation

mature form ending with GG

Promoter

Available since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only

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Dapp1-PH pET11a

Plasmid

#71376

Purpose

Phosphoinositide Binding

Depositor

Mark Lemmon

Article

Ferguson et al Mol Cell. 2000 Aug;6(2):373-84.

Insert

Dapp1-PH (DAPP1 Human)

Use

Tags

Expression

Bacterial

Mutation

Pleckstrin homology (PH) domain; aa 148-273

Promoter

T7

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only