-
Plasmid#154077PurposeExpresses MBP-GS 634-666-His (human 33aa Glycogen Synthase peptide as a fusion protein with MBP and His- tags) in E.coliDepositorInsertGYS1 (GYS1 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromoterTacAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDropIn-T2A-split-intein-Gal4[1-20]-N-int-BlastR
Plasmid#199197PurposeInsert to create split-intein Gal4[N-int] knock-in vectors, with blasticidin resistance, via Drop-In cloning, released via BsmBI digestDepositorInsertT2A-split-intein-Gal4[1-20]-N-int-BlastR
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDropIn-T2A-split-intein-Gal4[21-881]-C-int-G418R
Plasmid#199198PurposeInsert to create split-intein Gal4[21-881]-C-int knock-in vectors, with G418 resistance, via Drop-In cloning, released via BsmBI digestDepositorInsertT2A-split-intein-Gal4[21-881]-C-int-G418R
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
plexm-mouse LRRC33
Plasmid#111601PurposeExpress mouse LRRC33 in 293T cellsDepositorInsertmouse LRRC33 (Nrros Mouse)
UseTagsFLAGExpressionMammalianMutationStarts at aa 18PromoterAvailable sinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
N205 nLV EF-1a-ZFHD1-link-FKBP-HA <T2A> HP1aCS-Frbx2-V5-PGK-Blast
Plasmid#44017DepositorInsertsUseLentiviralTagsFrb, HA, Hu Frb x2(WT Frb+codon Wobble Frb), V5/e…ExpressionMutationN-term chromo domain removedPromoterEF-1aAvailable sinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
HNRNPH2_3xRRM_pGEX
Plasmid#135115PurposeRecombinant expression of HNRNPH2 with dual-affinity tagDepositorInsertHNRNPH2 (HNRNPH2 Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceOct. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
N163 nLV EF-1a-Hp1a(CS)-Frbx2(Frb+FrbWobb)-V5-PGK-Puro
Plasmid#44195DepositorInsertCbx5 (Cbx5 Mouse)
UseLentiviralTagsFrb, Hu Frb x2(WT Frb+codon Wobble Frb), and V5/e…ExpressionMutationN-term chromo domain removedPromoterEF-1aAvailable sinceJune 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
px335-EF-Slc2a3-L
Plasmid#122304PurposeExpresses sgRNA targeting mouse Slc2a3 and Cas9 nickase in mammalian cellsDepositorInsertsgRNA for mouse Slc2a3 (Slc2a3 Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMG023 MBP-Axin 384-518-His (miniAxin)
Plasmid#154012PurposeExpresses MBP-Axin_384-518-His (human Axin minimal scaffold fragment as a fusion protein with MBP and His- tags) in E.coliDepositorInsertAxin1 (AXIN1 Human)
UseTagsHis6 and MBPExpressionBacterialMutation381-518 fragmentPromoterTacAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
ILF2_DZF_pGEX
Plasmid#135162PurposeRecombinant expression of ILF2 with dual-affinity tagDepositorInsertILF2 (ILF2 Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceNov. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
3xNLS-NLP-cMyc-cMyc enAspCas12a
Plasmid#182127PurposepET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc enAspCas12a in bacteriaDepositorInsert3xNLS-NLP-cMyc-cMyc enAspCas12a
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
px335-sgRNA-EF(Cas9wt)-Rif1
Plasmid#122306PurposeExpresses sgRNA targeting mouse Rif1 and Cas9 in mammalian cellsDepositorInsertsgRNA for mouse Rif1 (Rif1 Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
CNOT4_RRM-ZNF_pGEX
Plasmid#135146PurposeRecombinant expression of CNOT4 with dual-affinity tagDepositorInsertCNOT4 (CNOT4 Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
ArpC3-FLAG_pLib
Plasmid#173680PurposeArpC3 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expressionDepositorInsertArpC3 (ARPC3 Human)
UseTagsTEV cleavage site and FLAG tagExpressionInsectMutationPromoterpolyhedrinAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
ArpC1_pLib
Plasmid#173678PurposeArpC1 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expressionDepositorInsertArpC1 (ARPC1B Human)
UseTagsExpressionInsectMutationPromoterpolyhedrinAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMG035 MBP-Axin∆BCD-His
Plasmid#154053PurposeExpresses MBP-Axin∆BCD-His (human Axin with aa465-518 deletion as a fusion protein with MBP and His- tags) in E.coliDepositorInsertAxin1 (AXIN1 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromoterTacAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
ET1-human LRRC33
Plasmid#111603PurposeExpress human LRRC33 in 293T cellsDepositorInserthuman LRRC33 (NRROS Human)
UseTagsPreScission/TEV/ProteinC/6xHIS/SBP/ExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_delta5C-PolyA
Plasmid#112289PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) delta5C mutant lacking the last five residues (FLTWL) in the PDZ binding domain at the N-terminus of the proteinDepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationHuman YAP1-2gamma lacking the last 5 Amino acids …PromoterEF-1 alphaAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
3xNLS-NLP-cMyc-cMyc AspCas12a
Plasmid#182122PurposepET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc AspCas12a in bacteriaDepositorInsert3xNLS-NLP-cMyc-cMyc AspCas12a
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-N23PP/L28F/G51PEKN
Plasmid#118585PurposeBacterial expression of E.coli DHFR N23PP, L28F, and G51PEKN mutantDepositorInsertDHFR
UseTagsExpressionBacterialMutationN23 mutated to P23, L28 mutated to F28, G51 mutat…PromoterT7Available sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
33-G-X1-pEF1-puro
Plasmid#111604PurposeExpress human 33-G-X1 in 293T cellsDepositorInserthuman LRRC33 and GARP chimeria (NRROS Human)
UseTagsFlag tagExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
RBM6_RRM_pGEX
Plasmid#135157PurposeRecombinant expression of RBM6 with dual-affinity tagDepositorInsertRBM6 (RBM6 Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
px335-EF-Slc2a3-R
Plasmid#122305PurposeExpresses sgRNA targeting mouse Slc2a3 and Cas9 nickase in mammalian cellsDepositorInsertsgRNA for mouse Slc2a3 (Slc2a3 Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
ArpC2_pLib
Plasmid#173679PurposeArpC2 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expressionDepositorInsertArpC2 (ARPC2 Human)
UseTagsExpressionInsectMutationPromoterpolyhedrinAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
BOLL_RRM_pGEX
Plasmid#135130PurposeRecombinant expression of BOLL with dual-affinity tagDepositorInsertBOLL (BOLL Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF086 MBP-CREB 127-135-His
Plasmid#154075PurposeExpresses MBP-CREB_127-135-His (human 9aa CREB peptide as a fusion protein with MBP and His- tags) in E.coliDepositorInsertCREB1 (CREB1 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromoterTacAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_Y357F-PolyA
Plasmid#112287PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) Y357F mutant _ corresponding to tyrosine 407 in this isoformDepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationTyrosine 357 to Phenyalanine corresponding to tyr…PromoterEF-1 alphaAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-PHD2
Plasmid#223552PurposeLentivirus transfer plasmid for expression of full length human PHD2DepositorInsertPHD2 (EGLN1 Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-puro_Panc480-MT7
Plasmid#200941PurposeMultiplexed CRISPR array expressing 7 sgRNA in a lentiviral backboneDepositorInsertmultiplexed sgRNA array
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6, EF-1aAvailable sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENN491_TALEcharm_MmT8x
Plasmid#220836PurposeTALEcharm Kv2 expression in mammalian cells; targets PrnpDepositorInsertTALEcharm
UseLentiviral and TALENTagsP2A-TagBFPExpressionMammalianMutationN/APromoterEF1aAvailable sinceJuly 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENN456_TALEcharm_Kv2_MmT8x
Plasmid#220835PurposeTALEcharm expression in mammalian cellsDepositorInsertTALEcharm Kv2
UseLentiviral and TALENTagsP2A-TagBFPExpressionMammalianMutationN/APromoterEF1aAvailable sinceJuly 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTMB398_ZFcharm_Prnp_SMM
Plasmid#220845PurposeAAV genome expressing ZFcharm targeting Prnp; single mismatch self-silencing binding siteDepositorInsertZFcharm
UseAAVTagsHAtagExpressionMammalianMutationN/APromoterEFSAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
cacna2d1 R241A pMT2
Plasmid#206110Purposeexpression of rat alpha2delta-1 calcium channel auxillary subunit and with R421A mutation that disrupts Gabapentin bindingDepositorInsertcacna2d1 (Cacna2d1 Rat)
UseTagsExpressionMammalianMutationR241APromoterAd MLP/TPL/SV40Available sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
cacna2d1 R241A HA pMT2
Plasmid#206111Purposeexpression of rat alpha2delta-1 calcium channel auxillary subunit with a single HA tag inserted after aa549 and with an R421A mutation that disrupts Gabapentin bindingDepositorInsertcacna2d1 (Cacna2d1 Rat)
UseTagsExpressionMammalianMutationR241APromoterAd MLP/TPL/SV40Available sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
p202-Syt1-C2A-I248V
Plasmid#201554PurposeExpresses Octopus synaptotagmin-1 C2A domain with I248V mutation in E. coliDepositorInsertSynaptotagmin-1 C2A domain (partial)
UseTagsExpressionBacterialMutationchanged Isoleucine 248 to Valine (numbering based…PromoterRBSAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-V5-HisB-ObKHC-K282R-HaloTag
Plasmid#201552PurposeExpresses Octopus kinesin-1 motor with K282R mutation with HaloTag in Drosophila S2 cellsDepositorInsertKinesin-1
UseTagsHaloTagExpressionInsectMutationchanged lysine 282 to arginine (numbering based o…PromoterAc5Available sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 BBS W391A pcDNA3
Plasmid#206077PurposeCav2.2 calcium channel with an exofacial double Bungarotoxin Binding Site motif in domain II and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop that prevents Beta bindingDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationW391APromoterCMVAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHD-T2A-split-intein-Gal4[1-20]-N-int
Plasmid#199203PurposeTo create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[1-20]N-intDepositorInsertT2A-split-intein-Gal4[1-20]-N-int
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHD-T2A-split-intein-Gal4[21-881]-C-int
Plasmid#199204PurposeTo create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[21-881]C-intDepositorInsertT2A-split-intein-Gal4[21-881]-C-int
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDropIn-T2A-split-intein-Gal4[1-20]-N-int
Plasmid#199195PurposeInsert to create split-intein Gal4[N-int] knock-in vectors via Drop-In cloning, released via BsmBI digestDepositorInsertT2A-split-intein-Gal4[1-20]-N-int, 3xP3-dsRed
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDropIn-T2A-split-intein-Gal4[21-881]-C-int
Plasmid#199196PurposeInsert to create split-intein Gal4[C-int] knock-in vectors via Drop-In cloning, released via BsmBI digestDepositorInsertT2A-split-intein-Gal4[21-881]-C-int, 3xP3-dsRed
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
L_NLS-mutNLS V5-Twist
Plasmid#201381PurposeExpresses Twist mutant for nucleocytoplasmic transport studiesDepositorInsertTwist (Twist1 Mouse)
UseTags(NLS)SV40A4-V5ExpressionMammalianMutationK38R K73RPromoterAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
M_NLS-mutNLS V5-Twist
Plasmid#201379PurposeExpresses Twist mutant for nucleocytoplasmic transport studiesDepositorInsertTwist (Twist1 Mouse)
UseTags(NLS)SV40A5-V5ExpressionMammalianMutationK38R K73RPromoterAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
mutNLS V5-Twist
Plasmid#201380PurposeExpresses Twist mutant for nucleocytoplasmic transport studiesDepositorInsertTwist (Twist1 Mouse)
UseTagsV5ExpressionMammalianMutationK38R K73RPromoterAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
H_NLS-mutNLS V5-Twist
Plasmid#201378PurposeExpresses Twist mutant for nucleocytoplasmic transport studiesDepositorInsertTwist (Twist1 Mouse)
UseTags(NLS)SV40-V5ExpressionMammalianMutationK38R K73RPromoterAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
UL_NLS-mutNLS V5-Twist
Plasmid#201382PurposeExpresses Twist mutant for nucleocytoplasmic transport studiesDepositorInsertTwist (Twist1 Mouse)
UseTags(NLS)SV40A3-V5ExpressionMammalianMutationK38R K73RPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
mut GBP2 V5-Twist
Plasmid#201377PurposeExpresses Twist mutant for nucleocytoplasmic transport studiesDepositorInsertTwist (Twist1 Mouse)
UseTagsV5ExpressionMammalianMutationY107EPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
Control V5-Twist
Plasmid#201376PurposeExpresses Twist for nucleocytoplasmic transport studiesDepositorInsertTwist (Twist1 Mouse)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-del942-971-GGSG_M
Plasmid#146950PurposeInsect Expression of DmAGO1-del942-971-GGSGDepositorInsertDmAGO1-del942-971-GGSG (AGO1 Fly)
UseTagsExpressionInsectMutationvariation with 2 non silent mutations V315A and H…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-del942-971-GSG_M
Plasmid#146951PurposeInsect Expression of DmAGO1-del942-971-GSGDepositorInsertDmAGO1-del942-971-GSG (AGO1 Fly)
UseTagsExpressionInsectMutationvariation with 2 non silent mutations V315A and H…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only