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Plasmid#183064PurposeBase editing plasmid for Pseudomonas chlororaphisDepositorInserteSpCas9ppD10A
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cas9m2
Plasmid#47314PurposeCas9 D10A+H840ADepositorInsertCas9m2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
Cas9m3
Plasmid#47315PurposeCas9 D10A+D839A+H840ADepositorInsertCas9m3
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMOD3-mClover-Sh_ble
Plasmid#120329PurposeBacterial mClover promoter trapDepositorInsertmClover
UseTagsExpressionBacterialMutationD103N (see comment below)Promoter(None)Available sinceAvailabilityAcademic Institutions and Nonprofits only -
ADAM10_Halo_C_allele
Plasmid#178151PurposeDonor vector for endogenous tagging of human ADAM10 at the C-terminus with halotagInsertHalotag (ADAM10 Human)
UseDonor vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMOD3-proD/mClover/nat
Plasmid#120330PurposeBacterial mClover labelingDepositorInsertmClover
UseTagsD103N (see comment below)ExpressionBacterialMutationPromoter(None)Available sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSET-dCas9
Plasmid#110183PurposeExpression of the dCas9 gene in StreptomycesDepositorInsertdCas9
UseCRISPR; ITagsExpressionBacterialMutationD10A, H840APromoterermE*pAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
Cas9m4
Plasmid#47316PurposeCas9 D10A+D839A+H840A+N863ADepositorInsertCas9m4
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMH220
Plasmid#122836PurposedCas9 with GreenGate B-C flanking sequencesDepositorInsertNuclease-dead SpCas9 (dCas9), codon-optimised for Arabidopsis
UseGolden gate compatible cloning vectorTagsExpressionMutationContains D10A and H840A mutationsPromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTPGI_dCas9
Plasmid#64279Purposeencodes yeast-optimized dCas9 synthetic transcription factorDepositorInsertYeast-optimized dCas9
UseTagsSV40 NLSExpressionYeastMutationD10A & H840A relative to S. pyogenes Cas9PromoterpTPGIAvailable sinceMay 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCSC-SP-PW-NepX (aka: pBOB-NEPX)
Plasmid#12340DepositorInsertNEP-X (MME Human)
UseLentiviralTagsExpressionMammalianMutationE585V (inactive mutant)PromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
p116 Mfz10a
Plasmid#11070DepositorInsertMfz10a (Fzd10 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
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ced-4S in pXHA
Plasmid#134970Purposeced-4S cDNA cloned into pXHA backbone, used for production of antibodiesDepositorInsertced-4 (ced-4 Nematode)
UseTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-dCas9
Plasmid#125687PurposeGene knockdown for actinomycetesDepositorInsertstreptomyces codon optimized spdCas9, sgRNA casette
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterermE*/tipAAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti-dCas9-KRAB-blast
Plasmid#89567PurposePlasmid expression dCas9 protein in fusion with KRAB domainDepositorInsertdCas9
UseLentiviralTagsBlasticidin resistance gene and KRABExpressionMutationD10A and H840APromoterAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWJ66
Plasmid#46570PurposeSame as pdCas9, but with the dCas9-w fusion (w is fused at the C-terminal end of dCas9)DepositorInsertstracrRNA
dcas9-w
CRISPR array
UseCRISPRTagsrpoZExpressionBacterialMutationD10A, H840APromoterAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKm-HCBE
Plasmid#170333Purposea modularized single-plasmid cytidine base editing (C-to-T) tool for Sinorhizobium melilotiDepositorInsertRhizobium codon-optimized CBEs
UseCRISPRTagsExpressionMutationCas9n-D10APromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKm-HGBE
Plasmid#170334Purposea modularized single-plasmid glycosylase base editing (C-to-G) tool for Sinorhizobium melilotiDepositorInsertRhizobium codon-optimized GBEs
UseCRISPRTagsExpressionMutationCas9n-D10APromoterAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKm-HABE
Plasmid#170332Purposea modularized single-plasmid adenosine base editing (A-to-G) tool for Sinorhizobium melilotiDepositorInsertRhizobium codon-optimized ABEs
UseCRISPRTagsExpressionMutationCas9n-D10APromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
Yeast_Target-AID3S
Plasmid#188646PurposeExpresses Target-AID3S in yeastsDepositorInsertnCas9-AID2S-UGI
UseTagsUGIExpressionYeastMutationD10A for SpCas9PromoterpGal1Available sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF-AncBE4max
Plasmid#138270PurposeExpression of AncBE4max-Cas9 Base-editor from an EF1a promoter.DepositorInsertAncBE4max
UseCRISPRTagsExpressionMammalianMutationD10APromoterEf1aAvailable sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBT374
Plasmid#125615PurposeExpresses evoFERNY-BE4max-NG in mammalian cellsDepositorInsertevoFERNY-BE4max-NG
UseTagsExpressionMammalianMutationH102P D104NPromoterAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC- VP64-dCas9-VP64-T2A- GFP(No LoxP sites)
Plasmid#66708Purposeexpresses VP64-dCas9-VP64, same plasmid as Addgene 59791 but with LoxP sites removedDepositorInsertS.Pyogenes VP64-dCas9-VP64
UseLentiviral and Synthetic BiologyTagsVP64ExpressionMutationD10A and H840APromoterhUbCAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBT280
Plasmid#122610Purposeexpress evoFERNY-BE4max in mammalian cellsDepositorInsertevoFERNY-BE4max
UseTagsExpressionMammalianMutationH102P D104NPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBJG1 T7-8xHis-OGT
Plasmid#154271PurposeBacterial expression of full length human OGT. N-terminal T7_leader-8xHis tag followed by HRV3C protease site.DepositorInsertO-GlcNAc Transferase (OGT Human)
UseTags8x His, HRV3C protease site, and T7 leaderExpressionBacterialMutationbacterial codon optimizedPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pScI_dCas9-CDA
Plasmid#108549PurposeBacterial Target-AID vectorDepositorInsertdCas9-PmCDA1
UseTagsFlagExpressionBacterialMutationD10A and H840A for SpCas9Promoterlambda ORAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
PX855
Plasmid#62887PurposeN-term SpCas9 piece of inducible transcriptional activator (dCas9(N)-FRB-2xNES)DepositorInsertSpCas9 (aa 2-535)
UseCRISPRTagsFRB and NES PTK2ExpressionMammalianMutationAspartic acide 10 to Alanine (D10A)PromoterCBhAvailable sinceMarch 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
Lv EF1a dCas9-HA 2A Blast
Plasmid#102812PurposeLentiviral plasmid from FIRE-Cas9 system to express dCas9-HA fusion proteinDepositorInsertdCas9-HA
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A and N863A (dCas9)PromoterEF1aAvailable sinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSET-dCas9-actII-4-NT-S1
Plasmid#110185PurposeRepression of gene expression in Streptomyces by CRISPRiDepositorInsertsdCas9
sgRNA
UseCRISPR; ITagsExpressionBacterialMutationD10A, H840APromoterJ23119 and ermE*pAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWJ68
Plasmid#46571PurposeSame as pdCas9, but with the w-dCas9 fusion (w is fused at the N-terminal end of dCas9)DepositorInsertstracrRNA
w-dcas9
CRISPR array
UseCRISPRTagsrpoZExpressionBacterialMutationD10A, H840APromoterAvailable sinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEF_dCas9
Plasmid#68416PurposeTransient expression of Sp-dCas9 in mammalian cells, under an EF1-alpha promoter.DepositorInsertdCas9
UseCRISPRTags3xFLAGExpressionMammalianMutationD10A/H841APromoterhuman EF1[alpha]Available sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
Epsin2-pmCherryC1
Plasmid#27673DepositorInsertEpsin 2 (Epn2 Mouse)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceMay 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-J23109
Plasmid#113151PurposePlasmid constitutively expressing dCas9 proteinDepositorInsertdCas9
UseTagsExpressionBacterialMutationD10A & H840APromoterAvailable sinceDec. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
EC2_7_dCas9_HC_sgRNA
Plasmid#163711PurposeHigh copy plasmid with dCas9 and sgRNA expression cassettesDepositorInsertdCas9
UseTagsExpressionYeastMutationPoint mutations D10A and H840APromoterScTEF1Available sinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
Mammalian_SaAID
Plasmid#188653PurposeExpresses SaAID in mammalian cellsDepositorInsertnSaCas9-AID-UGI
UseTagsUGIExpressionMammalianMutationD10A for SaCas9PromoterpCMVAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSL690
Plasmid#47753PurposeExpresses dCas9-VP64 fusionDepositorInsertdCas9-VP64
UseCRISPRTags3x FLAG, NLS, and VP64ExpressionMammalianMutationD10A and H840A mutations in Cas9PromoterCMVAvailable sinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEF-BE4Gam
Plasmid#138269PurposeExpression of BE4am-Cas9 Base-editor from an EF1a promoter.DepositorInsertBE4Gam
UseCRISPRTagsExpressionMammalianMutationD10APromoterEf1aAvailable sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
Mammalian_SaAID3S
Plasmid#188651PurposeExpresses SaAID3S and gRNA in mammalian cellsDepositorInsertsnSaCas9-AID3S--UGI
Sa-sgRNA
UseTagsUGIExpressionMammalianMutationD10A for SaCas9PromoterEF-1a and H1Available sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
Mammalian_SaAID2S
Plasmid#188652PurposeExpresses SaAID2S and gRNA in mammalian cellsDepositorInsertsAID2S-nSaCas9--UGI
Sa-sgRNA
UseTagsUGIExpressionMammalianMutationD10A for SaCas9PromoterEF-1a and H1Available sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF:iCas9
Plasmid#138267PurposeExpression of mTN3-GGS6-dCas9 (iCas9) in human.DepositorInsertmTN3-GGS6-dCas9
UseCRISPRTagsExpressionMammalianMutationCas9-D10A, H840APromoterEf1aAvailable sinceMarch 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pE2F3.3.1-gDNA
Plasmid#113776PurposeCRISPR plasmid for expression of Cas9 nickase (D10A) and gRNA targeting human transcription factor E2F3DepositorInsertE2F3 (E2F3 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc-MmUbc7/2776
Plasmid#11488DepositorInsertMmUbc7 (Ube2g2 Mouse)
UseTagsmycExpressionMammalianMutationn/aPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pQTEV-STX10
Plasmid#31572DepositorInsertsyntaxin 10 (STX10 Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc-MmUbc7mut/2777
Plasmid#11489DepositorInsertMmUbc7 (Ube2g2 Mouse)
UseTagsmycExpressionMammalianMutationcys 889 mutated to serPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGEX-KG-MmUbc7/2783
Plasmid#11490DepositorInsertMmUbc7 (Ube2g2 Mouse)
UseTagsGSTExpressionBacterialMutationn/aPromoterAvailable sinceApril 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
EC2_1_dCas9_sgRNA
Plasmid#163710PurposeYeast low copy plasmid with dCas9 and sgRNA expression cassetteDepositorInsertdCas9
UseTagsExpressionYeastMutationPoint mutations D10A and H840APromoterScTEF1Available sinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
peSpdCas9-J23109
Plasmid#113153PurposePlasmid constitutively expressing eSpdCas9 proteinDepositorInserteSpdCas9
UseTagsExpressionBacterialMutationD10A & K810A & H840A & K1003A & R…PromoterAvailable sinceDec. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF2G08
Plasmid#70915PurposeGateway entry cloneDepositorInsertParg (Parg Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceNov. 18, 2015AvailabilityAcademic Institutions and Nonprofits only