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Showing: 901 - 950 of 2028 results

  19. pCAG_SRGAP2A_GAP(R527L_K566A)

    Plasmid
    #222617
    Purpose
    Expresses full length SRGAP2 with R527L, K566A mt in the Rac1-specific GAP domain that abrogates the GTPase activating function of SRGAP2 without locking the GAP-domain in a form bound to Rac1-GDP.
    Depositor
    Franck Polleux
    Article
    Fossati et al Neuron. 2016 Jul 20;91(2):356-69. doi: 1
    Insert
    SRGAP2 (SRGAP2 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Point mutations R527L and K566A (Rac1-specific GA…
    Promoter
    Available since
    Oct. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    222617-plasmid-map-sequence-id-448408

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  36. ATP1A1_G6_T804N_Std_Dual_epegRNA_tevopreQ1

    Plasmid
    #187457
    Purpose
    Coselection for prime editing in human cells. Vector for tandem expression of ATP1A1 T804N-G6 pegRNA with a user-specified epegRNA. pU6-tevopreq1-GG-acceptor-like plasmid
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G6 T804N pegRNA (ATP1A1 Human)
    Use
    CRISPR; Prime editing
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tandem U6 promoters
    Available since
    July 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    187457-plasmid-map-sequence-id-367465

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  43. BPK1520_blastR

    Plasmid
    #175289
    Purpose
    Human expression plasmid for SpCas9 sgRNA with blasticidin co-selection
    Depositor
    Ronald Cohn
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV/U6
    Available since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    175289-plasmid-map-sequence-id-346344

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  44. CMV-pDisplay-Halo-T2A-EGFP

    Plasmid
    #223498
    Purpose
    Co-expression of HaloTags to outer surface in mammalian cells coupled with a EGFP transfection marker
    Depositor
    James Frank
    Insert
    HaloTag and EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    223498-plasmid-map-sequence-id-443020

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  45. gRNA telo/GFP

    Plasmid
    #137713
    Purpose
    Vector co-expressing sgRNA telo and GFP
    Depositor
    Dominik Niopek
    Insert
    sgRNA telo + GFP
    Use
    AAV, CRISPR, and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6/RSV
    Available since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    137713-plasmid-map-sequence-id-272438

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  46. MZB209

    Plasmid
    #228492
    Purpose
    BirA encoded pACYC vector for co-expression with Avi-tagged constructs (Bacterial Expression)
    Depositor
    Richard Baker
    Insert
    BirA (birA )
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228492-plasmid-map-sequence-id-455391

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  47. RedTrackCMV

    Plasmid
    #50957
    Purpose
    A backbone for adenovirus creation by the AdEasy method, similar to AdTrackCMV (Addgene Plasmid # 16405), except it co-expressed mRFP instead of eGFP
    Depositor
    James Bamburg
    Type
    Empty backbone
    Use
    Adenoviral
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    Feb. 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    50957-plasmid-map-sequence-id-76710

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  48. pMX-Flag-GFP_PDGFA

    Plasmid
    #227949
    Purpose
    Co-localisation experiment
    Depositor
    Alessandro Ori
    Insert
    PDGFA (PDGFA Human)
    Use
    Lentiviral
    Tags
    3xFLAG-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    227949-plasmid-map-sequence-id-455397

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  49. BII-Sh-BnTW

    Plasmid
    #133365
    Purpose
    Piggybac vector for shRNA-mediated knockdown, with co-expression with tdTomato-NLS
    Depositor
    Blair Madison
    Insert
    tdTomato-NLS
    Use
    Tags
    Flag-tagged tdTomato
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available since
    Jan. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    133365-plasmid-map-sequence-id-268571

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  50. pMX-Flag-GFP_PLEKHB1

    Plasmid
    #227952
    Purpose
    Co-localisation experiment
    Depositor
    Alessandro Ori
    Insert
    PLEKHB1 (PLEKHB1 Human)
    Use
    Lentiviral
    Tags
    3xFLAG-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    227952-plasmid-map-sequence-id-455409

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Showing: 901 - 950 of 2028 results