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pGEXNKI-his-GST-3C-LIC

Plasmid

#108709

Purpose

Expression of a GST-6xHis-tagged target protein with 3C protease cleavage site.

Depositor

Titia Sixma

Article

Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

Type

Empty backbone

Use

Tags

GST-6xHis-3C protease cleavage site

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pETNKI-his-3C-LIC-kan

Plasmid

#108703

Purpose

Expression of an His-tagged target protein with 3C protease cleavage site.

Depositor

Titia Sixma

Article

Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

Type

Empty backbone

Use

Tags

His-3C protease cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCDFNKI-his-3C-LIC-strep

Plasmid

#108705

Purpose

Expression of a His-tagged target protein with 3C protease cleavage site.

Depositor

Titia Sixma

Article

Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

Type

Empty backbone

Use

Tags

His-3C protease cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pETNKI-strepII-3C-LIC-kan

Plasmid

#108706

Purpose

Expression of a StrepII-tagged target protein with 3C protease cleavage site.

Depositor

Titia Sixma

Article

Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

Type

Empty backbone

Use

Tags

StrepII-3C protease cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pLacI-GFP-HEC1 WT

Plasmid

#114049

Purpose

Expression of exogenous LacI-GFP-HEC1 WT

Depositor

Geert Kops

Article

Nijenhuis et al J Cell Biol. 2013 Apr 15;201(2):217-31.

Insert

GFP-HEC1 (NDC80 Human)

Use

Tags

Expression

Mammalian

Mutation

WT HEC1 sequence

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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His-GINA3

Plasmid

#74980

Purpose

Expresses His tagged GINA3 protein in bacteria.

Depositor

Pradipta Ghosh

Article

Lin et al Mol Biol Cell. 2014 Nov 5;25(22):3654-71

Insert

Rattus guanine nucleotide binding protein alpha inhibiting 3 (Gnai3) (Gnai3 )

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

May 26, 2016

Availability

Academic Institutions and Nonprofits only

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pETNKI-his-3C-LIC-Amp

Plasmid

#108704

Purpose

Expression of a His-tagged target protein with 3C protease cleavage site.

Depositor

Titia Sixma

Article

Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

Type

Empty backbone

Use

Tags

His-3C protease cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pETNKI-strepII-3C-LIC-Amp

Plasmid

#108707

Purpose

Expression of a StrepII-tagged target protein with 3C protease cleavage site.

Depositor

Titia Sixma

Article

Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

Type

Empty backbone

Use

Tags

StrepII-3C protease cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pLacI-GFP-HEC1-d207

Plasmid

#114050

Purpose

Expression of exogenous LacI-GFP-HEC1 delta 207

Depositor

Geert Kops

Article

Nijenhuis et al J Cell Biol. 2013 Apr 15;201(2):217-31.

Insert

GFP-HEC1-d207 (NDC80 Human)

Use

Tags

Expression

Mammalian

Mutation

HEC1 delta 207

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-hygro-eGFP-HEC1-d207

Plasmid

#114051

Purpose

Expression of exogenous eGFP HEC1 delta 207

Depositor

Geert Kops

Article

Nijenhuis et al J Cell Biol. 2013 Apr 15;201(2):217-31.

Insert

eGFP-HEC1-d207 (NDC80 Human)

Use

Tags

Expression

Mammalian

Mutation

HEC1 delta 207

Promoter

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA5-LAP-MPS1-WT

Plasmid

#114044

Purpose

Expression of exogenous LAP tagged MPS1 WT

Depositor

Geert Kops

Article

Nijenhuis et al J Cell Biol. 2013 Apr 15;201(2):217-31.

Insert

MPS1-WT (TTK Human)

Use

Tags

Expression

Mammalian

Mutation

WT MPS1 sequence

Promoter

Available since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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GIV-F1685A FLAG

Plasmid

#65948

Purpose

Expression GIV/Girdin F1685A mutation in Mammalian cell

Depositor

Pradipta Ghosh

Article

Lin et al Mol Biol Cell. 2014 Nov 5;25(22):3654-71

Insert

GIV/Girdin (CCDC88A Human)

Use

Tags

3xFlag

Expression

Mammalian

Mutation

Phenylalanine 1685 to Alanine

Promoter

CMV

Available since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only
pCDNA5-LAP-MPS1-KD

Plasmid

#114045

Purpose

Expression of exogenous LAP tagged MPS1 Kinase-dead

Depositor

Geert Kops

Article

Nijenhuis et al J Cell Biol. 2013 Apr 15;201(2):217-31.

Insert

MPS1-KD (TTK Human)

Use

Tags

Expression

Mammalian

Mutation

D664A kinase dead

Promoter

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pET3a.H2B-K120C

Plasmid

#224706

Purpose

Expresses Human H2B K120C mutant in bacterial cells for fluorescent labelling of histone octamers

Depositor

Chen Davidovich

Article

Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

Insert

H2B-K120C (H2B Human)

Use

Tags

Expression

Bacterial

Mutation

K120C

Promoter

T7

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA5-LAP-MPS1-dTPR

Plasmid

#114048

Purpose

Expression of exogenous LAP tagged MPS1 delta TPR (TPR is deleted)

Depositor

Geert Kops

Article

Nijenhuis et al J Cell Biol. 2013 Apr 15;201(2):217-31.

Insert

MPS1-dTPR (TTK Human)

Use

Tags

Expression

Mammalian

Mutation

delta TPR (deletion of TPR domain 63-191)

Promoter

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA5-LAP-MPS1-d200

Plasmid

#114047

Purpose

Expression of exogenous LAP tagged MPS1 delta 200 (NTE and TPR are deleted)

Depositor

Geert Kops

Article

Nijenhuis et al J Cell Biol. 2013 Apr 15;201(2):217-31.

Insert

MPS1-d200 (TTK Human)

Use

Tags

Expression

Mammalian

Mutation

delta 200 (deletion of NTE and TPR domains)

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA5-LAP-MPS1-d60

Plasmid

#114046

Purpose

Expression of exogenous LAP tagged MPS1 delta 60 (NTE is deleted)

Depositor

Geert Kops

Article

Nijenhuis et al J Cell Biol. 2013 Apr 15;201(2):217-31.

Insert

MPS1-d60 (TTK Human)

Use

Tags

Expression

Mammalian

Mutation

delta 60 (deletion of NTE domain)

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe-EGFP-Hu_Plk1(WT)

Plasmid

#136323

Purpose

Retroviral expression of human Plk1-WT with N-terminal EGFP

Depositor

Marcos Malumbres, Guillermo de Cárcer

Insert

PLK1 (PLK1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.CBX8ΔChromo

Plasmid

#224708

Purpose

Expresses a human CBX8 truncation (deletion of the chromodomain) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag

Depositor

Chen Davidovich

Article

Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

Insert

CBX8 chromodomain truncation (CBX8 Human)

Use

Tags

HMBP

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pFBOH-MHL.mEGFP-CBX8

Plasmid

#224710

Purpose

Expresses N-terminal hexahistidine-tagged human CBX8 in insect cells, with a monomeric variant EGFP tag.

Depositor

Chen Davidovich

Article

Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

Insert

CBX8 (CBX8 Human)

Use

Tags

mEGFP

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

Oct. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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