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Plasmid#216361PurposexNup98-Nb1t (+1Cys) Nanobody productionDepositorInsertxNup98-Nb1t (+1Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
syntaxin3-myc-myc-His
Plasmid#12372DepositorInsertSyntaxin3 (STX3 Human)
UseTags2xMyc-HisExpressionMammalianMutationInsert is cloned into a modified vectorPromoterAvailable sinceSept. 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pX459-SpCas9-NG
Plasmid#171370PurposeSpCas9-NG-mediated genome editing.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6 for sgRNA; CBh for Cas9-2A-puroRAvailable sinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro-mTwist
Plasmid#1783DepositorInsertTwist (Twist1 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 10, 2005AvailabilityAcademic Institutions and Nonprofits only -
pMJ204: pU6-SpuFz1_omegaRNA(350nt)-entry
Plasmid#205268PurposeHuman expression vector for SpuFz1 omegaRNA 5' +275 nt extended scaffold (BbsI guide entry site)DepositorInsertSpuFz1 omegaRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
hRIP1 HA GFP wt
Plasmid#41396DepositorInsertRIP1 (RIPK1 Human)
UseTagsGFP and HAExpressionMammalianMutationE96GPromoterCMVAvailable sinceDec. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pegfp-N1-TFEB-S3A,R4A
Plasmid#44446DepositorInsertTFEB (TFEB Human)
UseTagsGFPExpressionMammalianMutationChanged both Serine 3 and Arginine 4 to AlaninePromoterCMVAvailable sinceApril 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDEST-hisMBP-AsCpf1-EC
Plasmid#79007PurposeExpressing his-MBP tagged AsCpf1 (E.coli codon optimized)DepositorInsertAsCpf1
UseTags6xHis-MBP-NLS and NLS-HAExpressionBacterialMutationPromotertac promoterAvailable sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
H2B-mNeonGreen-IRESpuro2
Plasmid#183745PurposeExpresses H2B-mNeonGreenDepositorInsertH2B (H2BC16P Human)
UseTagsmNeonGreenExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT-ChemoG-ATP
Plasmid#193818PurposeCMV overexpression of ChemoG-ATP in mammalian cell linesDepositorInsertChemoG-ATP
UseTagsExpressionMammalianMutationEGFP:A206K; HT7: L271EPromoterCMVAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
His-GFP-Syntaxin1A
Plasmid#170871PurposeMammalian expression of His-GFP-Syntaxin1A.DepositorInsertSyntaxin 1A (Stx1a Mouse)
UseTags(His)6 and EGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTetQCas-BsaI
Plasmid#170629PurposeExpression of CRISPR-associated transposasesDepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(BsaI)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
dsRed-cent2
Plasmid#29523DepositorInsertCent2 (CETN2 Human)
UseTagsdsRedExpressionMammalianMutationPromoterAvailable sinceMay 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
GFAP-Cre
Plasmid#24704DepositorInsertCre
UseTagsExpressionMammalianMutationPromoterGFAP (glial fibrillary acidic protein)Available sinceJuly 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCX-ThPDE5VV
Plasmid#134778PurposecGMP FRET biosensorDepositorInsertThPDE5VV
UseTagscpVenus-Venus and mTurquoise2ExpressionMammalianMutationPromoterCAGAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-mCherry-NLS
Plasmid#165442PurposeExpresses nuclear mCherry from CMV promoterDepositorInsertmCherry-NLS
UseAAVTagsNLSExpressionMammalianMutationPromoterCMVAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P34
Plasmid#120030PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 3xFLAGDepositorInsert3xFLAG
UsePcr templateTags3xFLAGExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
plk1 WT GFP-myc
Plasmid#132963PurposeTo study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damageDepositorInsertpolo-like kinase 1 (PLK1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2CG2--3.5ubb:plin2-tdtomato
Plasmid#172534PurposeZebrafish ubiquitin promoter driving zebrafish PLIN2-tdTOMATO fusion (lipid droplet reporter) with cmlc2:eGFP as a transgenesis markerDepositorInsert-3.5ubb:plin2-tdtomato
UseTagsExpressionMutationS80R, A81S, E144D, I148F and R415H mutations obseā¦Promoterzebrafish ubbAvailable sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only