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Plasmid#73421PurposeReporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4F2.DepositorInsertPromoter 4F2 (orthogonal T7-lac variant)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterP4F2 (orthogonal T7-lac variant)Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1REST_sh_seq2_WPRE
Plasmid#127574PurposeshRNA to knock down RE1-silencing transcription factor (REST)DepositorInsertshRNA for knock down of REST (REST Human)
UseLentiviralTagsExpressionMutationPromoterU6Available sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pGex-4T-2_LC3A
Plasmid#73946PurposeFor recombinant expression of human LC3A/MAP1LC3A in E. coliDepositorInsertLC3A (MAP1LC3A Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
Flag-hPKL WT
Plasmid#107159PurposeFor mammalian expression of Flag-hPKL WTDepositorInsertFlag-hPKL WT (PKLR Human)
UseRetroviralTagsN-term FlagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-REST_sh_seq1_WPRE
Plasmid#127573PurposeshRNA to knock down RE1-silencing transcription factor (REST)DepositorInsertshRNA for knock down of REST (REST Human)
UseLentiviralTagsExpressionMutationPromoterU6Available sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
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p2XStrep-ATIC
Plasmid#125685Purposeexpresses human ATIC protein with an N-terminal 2XStrep affinity tagDepositorInsertATIC (ATIC Human)
UseTags2X Strep-Tag IIExpressionMammalianMutationPromoterCMVAvailable sinceMay 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-FLuc-WPRE-HGHpA
Plasmid#87951PurposeRecombinant single-stranded AAV transfer vector expressing Firefly Luciferase under the EF1a promoterDepositorInsertFirefly Luciferase
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
MBP-rPKM2 WT
Plasmid#107158PurposeMBP-rPKM2 WT for production and purification of rat PKM2 WTDepositorInsertMBP-tagged rat pyruvate kinase M2 isoform (Pkm Rat)
UseTagsC-term 6-his and N-term MBPExpressionBacterialMutationPromoterPBADAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
BAF-WT-tet-puro
Plasmid#199648PurposeDoxycycline-inducible BAF expressionDepositorInsertBAF (BANF1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGex-4T-2_GATE-16
Plasmid#73518PurposeFor recombinant expression of human GATE-16/GABARAPL2 in E. coliDepositorInsertGATE-16 (GABARAPL2 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
FOR human OCT4 TALEN
Plasmid#52412PurposeTALEN expressing vector used to allow Knock-in of OCT4-GFP-2A-PURO knockin donor plasmid (generated by Jaenisch lab- Addgene Vector # 31939)DepositorInsertHD NG NN NN NN HD NG HD NG HD HD HD NI NG
UseTALENTagsExpressionMutationPromoterCAGGSAvailable sinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP mScarlet-MOSPD2 W201E
Plasmid#186477PurposeExpression of human MOSPD2 (mutant W201E, unable to bind lipid droplets) fused to mScarlet in mammalian cellsDepositorInsertMOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
UseRetroviralTagsmScarletExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pMG059 MBP-CREB-His
Plasmid#154076PurposeExpresses MBP-CREB-His (human CREB as a fusion protein with MBP and His- tags) in E.coliDepositorInsertCREB1 (CREB1 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromoterTacAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
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U6-sgfiller-eCas9-T2A-BlastR
Plasmid#172437PurposeExpresses eCas9, BlastR and sgRNA cloning siteDepositorInsertseCas9
BlastR
UseCRISPR, Lentiviral, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
MSCVhyg-HA-FLAG_Ubc9_WT
Plasmid#164936PurposeExpresses FLAG & HA tagged human uman Ubiquitin Conjugating Enzyme 9 (UBC9) wild type cDNADepositorInserthuman UBC9 (UBE2I Human)
UseRetroviralTagsFLAG & HA tagsExpressionMutationPromoterPGK promoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti-iStat3-BFP
Plasmid#216870PurposeLenti-iStat3-BFP plasmid contains the TRE (doxycycline inducible) promoter followed by the Stat3 mouse cDNA, a T2A and a Blue Fluorescent Protein (BFP) sequence.DepositorInsertStat3 (Stat3 Mouse)
UseLentiviralTagsT2A-TagBFPExpressionMammalianMutationPromoterTetracycline-Response-Element (TRE)Available sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only