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Plasmid#175751PurposeLentiviral vector expressing N-terminally tagged APEX2-FLAG-GABARAPL2DepositorInsertGABARAPL2 (GABARAPL2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV-Drosha(W1258)
Plasmid#171535PurposeEncodes mutant human Drosha proteinDepositorInsertDROSHA (DROSHA Human)
UseTagsExpressionMammalianMutationa Drosha stopping at W1258PromoterAvailable sinceOct. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV-Drosha (P426)
Plasmid#171537PurposeEncodes mutant human Drosha proteinDepositorInsertDROSHA (DROSHA Human)
UseTagsExpressionMammalianMutationa Drosha mutant starting from P426PromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV-Drosha (E419)
Plasmid#171534PurposeEncodes mutant human Drosha proteinDepositorInsertDROSHA (DROSHA Human)
UseTagsExpressionMammalianMutationa Drosha mutant stopping at E419PromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCK-Drosha-FLAG(delta6)
Plasmid#171532PurposeEncodes mutant human Drosha proteinDepositorInsertDROSHA (DROSHA Human)
UseTagsExpressionMammalianMutationsmall deletions of Drosha_1271A to 1276G_PromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pLenti6.2-coHPDL H258A 3xFlag-V5
Plasmid#174130PurposeExpresses codon-optimized catalytically inactive HPDL with a 3xFlag-V5 tag in mammalian cellsDepositorInsert4-hydroxyphenylpyruvate dioxygenase like (HPDL Human)
UseLentiviralTags3xFLAG-V5ExpressionMammalianMutationCodon optimized, H258APromoterAvailable sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag-gp130dimer-stm2585
Plasmid#174395PurposeConstitutively active gp130 dimer with codon-optimized stm2585 insert (replacing amino acids 140-179)DepositorInsertIL6ST cytosolic domain and sarA chimera
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag-stm2585-gp130
Plasmid#174393PurposeChimera of codon-optimized stm2585 (sarA/steE) and human gp130 sequence.DepositorInsertsarA-IL6ST chimera
UseTagsFlagExpressionMammalianMutation140-177 of sarA replaced with homologous IL6ST se…PromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUASTattB-2xFlag-mCherry-Msp300KASH
Plasmid#170807PurposeExpresses Msp300 KASH domain fused to 2xFLAG and mCherry under Gal4/UAS control for Drosophila transgenesisDepositorInsert2xFLAG/mCherry-Msp300KASH
UseTags2xFLAG-mCherry and Msp300KASHExpressionInsectMutationPromoter5xUAS + hsp70 minimal promoterAvailable sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLAG-PGRMC1-1-170
Plasmid#173467PurposePGRMC1 lacking the extreme C-terminusDepositorInsertPGRMC1 (PGRMC1 Human)
UseTagsFLAGExpressionMammalianMutationDeleted for amino acids 171-194PromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLAG-PGRMC-Hybrid1
Plasmid#173469PurposeN-terminal region of PGRMC2 fused to the transmembrane and C-terminal region of PGRMC1DepositorUseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLAG-PGRMC-Hybrid2
Plasmid#173470PurposeN-terminal and transmembrane region of PGRMC2 fused to the C-terminal region of PGRMC1DepositorUseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLAG-PGRMC-Hybrid3
Plasmid#173471PurposeN-terminal region of PGRMC1 fused to the transmembrane and C-terminal region of PGRMC2DepositorUseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV-Drosha(R871)
Plasmid#171536PurposeEncodes mutant human Drosha proteinDepositorInsertDROSHA (DROSHA Human)
UseTagsExpressionMammalianMutationa truncation at R871. Please see depositor commen…PromoterAvailable sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCI-Flag-NEDD4L∆WW4
Plasmid#166710Purposeexpresses NEDD4L with inactivating mutation in fourth WW domain in mammalian cellsDepositorInsertNEDD4L W576A
UseTagsFLAGExpressionMammalianMutationchanged tryptophan 576 to alaninePromoterCMV promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCI-Flag-NEDD4L∆WW1
Plasmid#166707Purposeexpresses NEDD4L with inactivating mutation in first WW domain in mammalian cellsDepositorInsertNEDD4L W221A
UseTagsFLAGExpressionMammalianMutationchanged tryptophan 221 to alaninePromoterCMV promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
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Flag-M4-AR-W741C-S81A-P82A
Plasmid#171250PurposeMammalian expression of 3xFlag-tagged human androgen receptor Serine-Proline mutant that binds to bicalutamide: AR-Trp741Cys-Ser81Ala-Pro82Ala (AR-W741C-S81A-P82A)DepositorInsert3xFlag-tagged human androgen receptor Trp741Cys-Ser81Ala-Pro82Ala mutant (AR Human)
UseTagsFlagExpressionMammalianMutationAR-Trp741Cys-Ser81Ala-Pro82Ala (AR-W741C-S81A-P82…PromoterCMVAvailable sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
Flag-M4-AR-W741C-S81A-P82G
Plasmid#171251PurposeMammalian expression of 3xFlag-tagged human androgen receptor Serine-Proline mutant that binds to bicalutamide: AR-Trp741Cys-Ser81Ala-Pro82Gly (AR-W741C-S81A-P82G)DepositorInsert3xFlag-tagged human androgen receptor Trp741Cys-Ser81Ala-Pro82Gly mutant (AR Human)
UseTagsFlagExpressionMammalianMutationAR-Trp741Cys-Ser81Ala-Pro82Gly (AR-W741C-S81A-P82…PromoterCMVAvailable sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only