Addgene

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pGGF000

Plasmid

#48861

Purpose

Empty entry vector for creating GreenGate plant resistance cassette modules.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pJKW0825

Plasmid

#119022

Purpose

E.coli expression plasmid for Pm4CL1

Depositor

Jing-Ke Weng

Article

Pluskal et al Nat Plants. 2019 Jul 22. pii: 10.1038/s4

Insert

Pm4CL1

Use

Tags

8xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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PX398 Nitratifractor salsuginis str DSM 16511 Cas9

Plasmid

#68334

Purpose

Nitratifractor salsuginis str DSM 16511 Cas9

Depositor

Feng Zhang

Insert

NsCas9

Use

CRISPR

Tags

HA and NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pJKW1574

Plasmid

#119178

Purpose

E.coli expression plasmid for Pm4CL1 and PmSPS1

Depositor

Jing-Ke Weng

Article

Pluskal et al Nat Plants. 2019 Jul 22. pii: 10.1038/s4

Inserts

Pm4CL1
PmSPS1

Use

Tags

Expression

Bacterial

Mutation

Promoter

pGAP

Available since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pGSH0

Plasmid

#45500

Depositor

Nelson Lau

Article

Zeng et al Methods. 2013 May 23. pii: S1046-2023(13

Type

Empty backbone

Use

RNAi

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 18, 2013

Availability

Academic Institutions and Nonprofits only

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pGGC051

Plasmid

#48832

Purpose

Provides GUS as GreenGate coding sequence module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

GUS

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pJKW1659

Plasmid

#119180

Purpose

E.coli expression plasmid for Pm4CL1 and PmCHS

Depositor

Jing-Ke Weng

Article

Pluskal et al Nat Plants. 2019 Jul 22. pii: 10.1038/s4

Inserts

Pm4CL1
PmCHS

Use

Tags

Expression

Bacterial

Mutation

Promoter

pGAP

Available since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pGGC015

Plasmid

#48828

Purpose

Provides mCherry as GreenGate coding sequence module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

mCherry

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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Landing_Pad_8xCTS-ECFP-pA

Plasmid

#200242

Purpose

Backbone for cloning 8xCTSs (8xCRISPR-target sites)

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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pJKW1547

Plasmid

#119176

Purpose

Yeast expression plasmid for PmSPS2

Depositor

Jing-Ke Weng

Article

Pluskal et al Nat Plants. 2019 Jul 22. pii: 10.1038/s4

Insert

PmSPS2

Use

Tags

Expression

Yeast

Mutation

Promoter

TEF1

Available since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pJKW1548

Plasmid

#119177

Purpose

Yeast expression plasmid for PmCHS

Depositor

Jing-Ke Weng

Article

Pluskal et al Nat Plants. 2019 Jul 22. pii: 10.1038/s4

Insert

PmCHS

Use

Tags

Expression

Yeast

Mutation

Promoter

TEF1

Available since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pJKW1077

Plasmid

#119025

Purpose

E.coli expression plasmid for PmCHS

Depositor

Jing-Ke Weng

Article

Pluskal et al Nat Plants. 2019 Jul 22. pii: 10.1038/s4

Insert

PmCHS

Use

Tags

8xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pJKW1413

Plasmid

#119174

Purpose

Yeast expression plasmid for Pm4CL1

Depositor

Jing-Ke Weng

Article

Pluskal et al Nat Plants. 2019 Jul 22. pii: 10.1038/s4

Insert

Pm4CL1

Use

Tags

Expression

Yeast

Mutation

Promoter

TEF1

Available since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pJKW1538

Plasmid

#119175

Purpose

Yeast expression plasmid for PmSPS1

Depositor

Jing-Ke Weng

Article

Pluskal et al Nat Plants. 2019 Jul 22. pii: 10.1038/s4

Insert

PmSPS1

Use

Tags

Expression

Yeast

Mutation

Promoter

TEF1

Available since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pGGC024

Plasmid

#48829

Purpose

Provides BFP as GreenGate coding sequence module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

BFP

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pSFFV_mNG2(11)1-10

Plasmid

#82610

Purpose

Lentiviral vector of constitutive expression of mNeonGreen2(1-10)

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

mNeonGreen2(1-10)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

K128M, S142T, R150M, G172V and K213M

Promoter

SFFV

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_sfCherry2(1-10)

Plasmid

#82602

Purpose

Expresses sfCherry2(1-10) fusion in mammalian cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

sfCherry2(1-10)

Use

Tags

Expression

Mammalian

Mutation

changed Glu 118 to Gln, changed Thr 128 to Ile

Promoter

CMV

Available since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pICSL30008

Plasmid

#50300

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

N terminal HA tag (6x Human influenza hemagglutinin)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2R

Plasmid

#167932

Purpose

lentiCRISPRv2 variant with mScarlet-I fluorescence marker.

Depositor

Ronald Germain

Article

Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6, EF1a

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCool-mRbx1-His-hCul1-CTP

Plasmid

#29519

Depositor

Ning Zheng

Insert

Rbx1 CUL1 (CUL1, Rbx1 Mouse, Human)

Use

Tags

GST, His (before thrombin), and Thrombin Cut Site…

Expression

Bacterial

Mutation

Promoter

Available since

May 26, 2011

Availability

Academic Institutions and Nonprofits only