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Plasmid#171134PurposereporterDepositorInsertRBM8A 3'UTR (RBM8A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmirGlo-RBM8A-3'UTR AluY-Sz WT 2598-4066
Plasmid#171136PurposereporterDepositorInsertRBM8A 3'UTR (RBM8A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
FUS_LC_charge_RtoK
Plasmid#139127Purposeexpress FUS LC with hnRNPA2-like charge patterning where all R are changed to KDepositorInsertFUS (FUS Human)
UseTagsExpressionBacterialMutationAdd K and D to FUS LC so it has the same charge p…PromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
TPC2(E643A)-mCherry
Plasmid#135187PurposeMutant TPC2 with reduced Ca2+ selectivity, tagged on C terminus with mCherry.DepositorInsertTPC2 (Tpcn2 Mouse)
UseLentiviralTagsmCherryExpressionMammalianMutationMutation of glutamate 643 to alaninePromoterAvailable sinceDec. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIE-N-hα2M-H6
Plasmid#128493PurposeExpresses human N-α2M (from S24 to E908) in insect cells. With C-t H6x tag.DepositorInsertA2M (S24-E908) (A2M Human)
UseTagsHis tag (6x)ExpressionInsectMutationPromoterIE1Available sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pS6-N-hα2M-H6
Plasmid#128496PurposeExpresses human N-α2M (from G27 to A788) in mammalian cells. With C-t H6x tag.DepositorInsertA2M (G27-A788) (A2M Human)
UseTagsHis tag (6x)ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
frameshift ELL2
Plasmid#127257PurposeFor in vitro translation of human a frameshift ELL2 that results in a 58 kDa product from internal initiation.DepositorInsertELL2 (ELL2 Human)
UseIn vitro translationTagsExpressionMutationXhoI site between the 1st &2nd Met was filled…PromoterT7Available sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
564aa ELL2 bluescript
Plasmid#127267PurposeFor in vitro translation of human ELL2 lacking COOH regionDepositorInsertELL2 (ELL2 Human)
UseIn vitro translationTagsExpressionMutationAddgene Plasmid 127256--SphI site filled in, then…PromoterT7Available sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJet-CMV-CD52-bglpA
Plasmid#89704PurposeHuman CD52 expression plasmid with short polyADepositorInsertCMV-CD52-bglpA (CD52 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_nmPKA-CαN-mPAGFP
Plasmid#114130PurposeMammalian expression of the G2A mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorInsertthe N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP, with G2A mutation (Prkaca Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHDT2:GUS
Plasmid#108444PurposeArabidopsis transformation, expresses HDT2 promoter/GUS fusion to study expression pattern in rootsDepositorInsertHDT2 promoter (HD2B Mustard Weed)
UseTagsGUSExpressionPlantMutationPromoterHDT2Available sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHDT2:HDT2-GFP
Plasmid#108566PurposeArabidopsis transformation, expresses HDT2/GFP fusion with HDT2 promoter to study expression pattern in rootsDepositorInsertHDT2 (HD2B Mustard Weed)
UseTagsGFPExpressionPlantMutationPromoterHDT2Available sinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR1-Nterm 6xHis PL K16TAG-T500
Plasmid#92315PurposeExpression of NTerm 6xHis tagged protein L (PL) mutant K16TAG for ncAA incoporation using the p70a promoter and UTR1DepositorInsertB1 domain of protein L (PL) mutant K16TAG
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialMutationPromoterp70b (p70a promoter with one mutation)Available sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70b-UTR1-NTerm 6xHis-zmADH-T500
Plasmid#92229PurposeExpression of zmADH using the p70b (weak version of the p70a promoter) promoter and UTR1DepositorInsertNTerm 6xHis Tagged zmADH
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialMutationPromoterp70b (p70a promoter with one mutation)Available sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMIR-192-Reporter
Plasmid#71872PurposeMammalian expression vector for the analysis of miR-19α activityDepositorInsertReverse complementary sequence of miR-192
UseTagsfirefly luciferaseExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMIR-193a-5p-Reporter
Plasmid#71873PurposeMammalian expression vector for the analysis of miR-193a-5p activityDepositorInsertReverse complementary sequence of miR-193a-5p
UseTagsfirefly luciferaseExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMIR-31-192-193a-Reporter
Plasmid#71874PurposeMammalian expression vector for the analysis of miR-31-19α-193a activityDepositorInsertBinding sequence targeted by miR-31, miR192, and miR-193a-5p
UseTagsfirefly luciferaseExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF4D03
Plasmid#70969PurposeGateway entry cloneDepositorInsertTaf12 (Taf12 Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceDec. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_CUS1F03
Plasmid#71071PurposeGateway entry cloneDepositorInsertSu(var)205 (Su(var)205 Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceNov. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUAST-HA, Pbl-A C-Term
Plasmid#69795PurposePebble isoform A, C-Term, in P element-based pUAST vector for Gal4-regulated expression in Drosophila.DepositorInsertPebble C-Term domain (Drosophila guanine nucleotide exchange factor)
UseP element-based puast vector for gal4-regulated e…TagsHA-TagExpressionInsectMutationJust the C-Term domain of Pebble (pbl)Promoterhsp70 promoterAvailable sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only