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Plasmid#140753PurposeFRET-Based Genetically Encoded Nanosensor for Lactate/Pyruvate Ratio, Negative ControlDepositorInsertDead-Lapronic
UseTagsExpressionMammalianMutationChanged Histidine 92 to AlaninePromoterCMVAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGFP-Rab8A[T22N]
Plasmid#24899DepositorInserthRab8A[T22N]
UseTagsGFPExpressionMammalianMutationChanged Threonine 22 to AsparaginePromoterAvailable sinceJune 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBITE-EGFRvIII-F263-OKT3
Plasmid#190680PurposeEncodes an EGFRvIII-CD3 BITE protein (F263 clone)DepositorInsertAnti-EGFRvIII-scFv-F263-modularized-OKT3-HisTag
UseLentiviralTagsmNeonGreenExpressionMammalianMutationPromoterEFSAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
C1-MPAct-mCherry
Plasmid#155222PurposeMonitor changes in the density of membrane-proximal F-actin (MPA)DepositorInsertF-tractin (aa 9-52 of rat ITPKA) fused to mCherry C-term tagged with the CaaX sequence derived from Kras4b
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pM122: pAAV-EFS-CasRx
Plasmid#166870PurposeAAV vector for expressing CasRxDepositorInsertRfxCas13d
UseAAV and CRISPRTagsHA and NLSExpressionMammalianMutationPromoterEFSAvailable sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT-ChemoD-NAD
Plasmid#193826PurposeCMV overexpression of ChemoD-NAD in mammalian cell linesDepositorInsertChemoD-NAD
UseTagsExpressionMammalianMutationShadowG: T225R, HT7: L271EPromoterCMVAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
Ki67-mNeonGreen-IRESpuro2
Plasmid#183737PurposeExpresses Ki67-mNeonGreenDepositorInsertMKI67 (MKI67 Human)
UseTagsmNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
alpha7nACHR_Nacho_GCAMP7s
Plasmid#182940Purposealpha7 nACHR expression in mammalian cell lines with calcium reporterDepositorInsertNACH7 (CHRNA7 Human)
UseTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
vGAT-pH pcaggs SE
Plasmid#78578Purposeimage recycling of VGAT in live neuronsDepositorInsertvGAT-pHluorin C-term (Slc32a1 Rat)
UseTagssuperecliptic pHluorinExpressionMammalianMutationPromoterchicken actinAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPEP-TEV cNup98 (1-498)
Plasmid#38037DepositorInsertNucleoporin 98 (NUP98 Human)
UseTagsHis-tag, 3CysteineExpressionBacterialMutationamino acids 1-498PromoterT7Available sinceSept. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
mAzurite-16xRhoBAST
Plasmid#196347PurposeExpression of mAzurite mRNA with 16xRhoBAST in 3'UTRDepositorInsertmAzurite-16xRhoBAST
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUGW-d2GFP-200
Plasmid#79602PurposeFUGW lentiviral vector with constiutively expressed destabilized GFP (d2GFP) regulated by 5 miR-200 family binding sitesDepositorInsertd2GFP + miR-200 family targets
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
7i sgRNA for 4-μHOM reporter
Plasmid#113626PurposesgRNA/CAS9 expression plasmid to induce a 3’ double-strand break in the 4-μHOM reporter 8 nts. upstream from the microhomologyDepositorInsert7i sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
SLF063: Lenti-fCAG-jRGECO1a
Plasmid#124775PurposeFor general lentiviral or direct expression of cytosolic jRGECO1aDepositorInsertjRGECO1a
UseLentiviralTagsNES-6xHISExpressionMammalianMutationPromoterCAGAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpyTag-β-Lactamase-SpyCatcher
Plasmid#52656PurposeExpresses SpyTag-SpyCatcher mediated cyclized version of TEM-1 β-lactamase (BLA). Cyclization of BLA led to an increase in thermal resilience of >60 degrees celsius.DepositorInsertTEM-1 β-Lactamase
UseTagsSpyCatcher and SpyTagExpressionBacterialMutationPromoterT7 promoterAvailable sinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
7xE-Box::Renilla
Plasmid#124532Purposec-Myc Renilla luciferase reporterDepositorInsertc-Myc Renilla reporter
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBad-mCherry-XL (R125K)
Plasmid#188054PurposeExpresses His-tagged, Arabinose-inducible mCherry-XL (R125K) in bacteriaDepositorInsertmCherry-XL (R125K)
UseTags6xHisExpressionBacterialMutationdiffers from mCherry by R125K, W143S, I161V, Q163…PromoteraraBADAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pC008 - pBR322 with tetR-inducible RFP
Plasmid#79157Purposeinducible RFP expression vectorDepositorInsertTetR + RFP
UseTagsExpressionMutationPromoterAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBBK95 pCAS-Tyr-[gRNA: 2xBsaI+NotI cut sites] (HygR)
Plasmid#179079PurposeSp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Hygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
C1-MPAct-mCitrine
Plasmid#155220PurposeMonitor changes in the density of membrane-proximal F-actin (MPA)DepositorInsertF-tractin (aa 9-52 of rat ITPKA) fused to mCitrine C-term tagged with the CaaX sequence derived from Kras4b
UseTagsmCitrineExpressionMammalianMutationPromoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only