Skip to main content
Addgene
Showing: 3741 - 3760 of 12906 results

  8. pCEP4_HLA-flag-GRM3 C127A-VN

    Plasmid
    #140449
    Purpose
    Mammalian expression plasmid for FLAG-tagged mGluR3 fused to the N-terminal half of split fluorescent Venus (VN)
    Depositor
    Erik Procko
    Insert
    GRM3 (GRM3 Human)
    Use
    Tags
    FLAG tag, Signal/leader sequence from HLA class I…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine
    Promoter
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    140449-plasmid-map-sequence-id-292293

    Enlarge

  9. pORANGE_NFL linker(A6TAG)-3xFLAG KI

    Plasmid
    #182680
    Purpose
    Expression of spCas9, gRNA targeting the end of mouse Nefl gene and donor linker(A6TAG)-3xFLAG. Can be used for amber codon suppression and click chemistry labeling of endogenous NFL.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    Nefl-targeting gRNA and linker(A6TAG)-3xFLAG donor sequence (Nefl Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and chicken beta-actin promoter
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182680-plasmid-map-sequence-id-363164

    Enlarge

  10. pN23-LIC-FLAG-STREP

    Plasmid
    #182508
    Purpose
    pET based vector for protein expression in E.coli for targets fused with N-terminal Flag and STREP
    Depositor
    Renato Bruni
    Type
    Empty backbone
    Use
    Tags
    STREP-FLAG-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    182508-plasmid-map-sequence-id-361393

    Enlarge

  11. pNYCOMPS-C-Lic-His-FLAG

    Plasmid
    #182505
    Purpose
    pET based vector for protein expression in E.coli for targets fused with C-terminal Flag and His
    Depositor
    Renato Bruni
    Type
    Empty backbone
    Use
    Tags
    TEV-FLAG-10x His
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    182505-plasmid-map-sequence-id-361391

    Enlarge

  12. pMT-FLAG-CBPΔ(N+HAT)

    Plasmid
    #182841
    Purpose
    An EcoRV fragment (encoding residues 1566-2142) was removed from pMT-FLAG-CBPΔN. Expression in S2 cells by CuSO4 induction.
    Depositor
    Peter Harte
    Article
    Tie et al Development. 2009 Sep;136(18):3131-41. d
    Insert
    CBP (w Fly)
    Use
    Tags
    FLAG tag
    Expression
    Insect
    Mutation
    dletion of residues 1566-2142 from CBP1476-3222
    Promoter
    MT
    Available since
    April 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    182841-plasmid-map-sequence-id-360051

    Enlarge

  14. pNYCOMPS-N-Lic-His-FLAG

    Plasmid
    #182506
    Purpose
    pET based vector for protein expression in E.coli for targets fused with N-terminal Flag and His
    Depositor
    Renato Bruni
    Type
    Empty backbone
    Use
    Tags
    10xHis-FLAG-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    182506-plasmid-map-sequence-id-358073

    Enlarge

  17. pCMV-Armc12/FLAG/BioID2/HA

    Plasmid
    #180498
    Purpose
    Expression vector of BirA-fused mouse Armc12. HA-tagged BirA was linked to the FLAG-tagged Armc12. See Fig.S6 of Shimada, K. et al. Proc. Natl. Acad. Sci. USA. 118 (6): e2018355118, 2021.
    Depositor
    Masahito Ikawa
    Article
    Shimada et al Proc Natl Acad Sci U S A. 2021 Feb 9;118
    Insert
    armadillo repeat containing 12 (Armc12 Mouse)
    Use
    Tags
    FLAG tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    March 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    180498-plasmid-map-sequence-id-356916

    Enlarge

  18. pCAG1.1-Armc12 delta N/FLAG

    Plasmid
    #180495
    Purpose
    Expression vector of N-terminal deleted mouse Armc12 tagged with FLAG at C-terminus. See Fig.4D of Shimada, K. et al. Proc. Natl. Acad. Sci. USA. 118 (6): e2018355118, 2021.
    Depositor
    Masahito Ikawa
    Article
    Shimada et al Proc Natl Acad Sci U S A. 2021 Feb 9;118
    Insert
    armadillo repeat containing 12 (Armc12 Mouse)
    Use
    Tags
    FLAG tag
    Expression
    Mammalian
    Mutation
    N-terminus of Armc12 is deleted
    Promoter
    CAG promoter
    Available since
    March 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    180495-plasmid-map-sequence-id-356914

    Enlarge

  19. pCAG1.1-Armc12 delta C/FLAG

    Plasmid
    #180497
    Purpose
    Expression vector of C-terminal deleted mouse Armc12 tagged with FLAG at C-terminus. See Fig.4D of Shimada, K. et al. Proc. Natl. Acad. Sci. USA. 118 (6): e2018355118, 2021.
    Depositor
    Masahito Ikawa
    Article
    Shimada et al Proc Natl Acad Sci U S A. 2021 Feb 9;118
    Insert
    armadillo repeat containing 12 (Armc12 Mouse)
    Use
    Tags
    FLAG tag
    Expression
    Mammalian
    Mutation
    C-terminus of Armc12 is deleted
    Promoter
    CAG promoter
    Available since
    March 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    180497-plasmid-map-sequence-id-356912

    Enlarge

  20. pCAG-mPpp3cc-FLAG-6XHIS

    Plasmid
    #179160
    Purpose
    Expression vector of mouse protein phosphatase 3, catalytic subunit, gamma isoform (Ppp3cc) tagged with FLAG and 6X HIS at C-terminus, CAG promoter, rabbit globin poly(A) signal.
    Depositor
    Masahito Ikawa
    Article
    Miyata et al Science. 2015 Oct 23;350(6259):442-5. do
    Insert
    protein phosphatase 3, catalytic subunit, gamma isoform (Ppp3cc Mouse)
    Use
    Tags
    6XHIS and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179160-plasmid-map-sequence-id-352799

    Enlarge

Showing: 3741 - 3760 of 12906 results