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Plasmid#40142DepositorInsertpar-2 RNAi resistant (R183-5A + S241A) (par-2 Nematode)
UseGateway destination vectorTagsGFPExpressionWormMutationR183-5A + S241A; base pairs 6-523 of par-2 were …Promoterpie-1Available sinceSept. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFM036
Plasmid#40139DepositorInsertpar-2 RNAi resistant (K162A) (par-2 Nematode)
UseGateway destination vectorTagsGFPExpressionWormMutationK162A; base pairs 6-523 of par-2 were recoded to…Promoterpie-1Available sinceSept. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pRLCon1288-1666
Plasmid#31448DepositorInsertHNF4A 3'UTR fragment (HNF4A Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRLCon183-221_850-1207
Plasmid#31449DepositorInsertHNF4A 3'UTR fragments (HNF4A Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRLCon183-221mut2_850-1207
Plasmid#31450DepositorInsertHNF4A 3'UTR fragments (HNF4A Human)
UseLuciferaseTagsExpressionMammalianMutationmut2 in balancerPromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRLCon1-449_850-1207
Plasmid#31451DepositorInsertHNF4A 3'UTR fragments (HNF4A Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV/D377Y-mPCSK9
Plasmid#58376PurposeExpresses GoF mutant murine PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosisDepositorInsertproprotein convertase subtilisin/kexin type 9 (Pcsk9 Mouse)
UseAAVTagsExpressionMammalianMutationD377YPromoterHCRApoE/hAATAvailable sinceAug. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
STARR-seq luciferase validation vector_ORI_neg.cont
Plasmid#99315PurposeNegative control for luciferase validation vectorsDepositorInsertneutral region; chr3: 55198740-55200041
UseLuciferase; Starr-seq luciferase validation vectorTagsExpressionMammalianMutationPromoterAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
Lamp1-RFP
Plasmid#1817PurposeMammalian expression of Lamp1 fused to RFP. Used for localization to the lysosome.DepositorInsertlysosome associated membrane protein 1 (Lamp1 Rat)
UseTagsRFPExpressionMammalianMutationD50E (not important for function of plasmid)PromoterAvailable sinceSept. 26, 2005AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Frankenbody-FLAG-mEGFP
Plasmid#175293PurposeTrack mature and nascent FLAG tagged proteins in live cellsDepositorInsertanti-FLAG frankenbody fused with mEGFP
UseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Frankenbody-FLAG-Halo
Plasmid#175296PurposeTrack mature and nascent FLAG tagged proteins in live cellsDepositorInsertanti-FLAG frankenbody fused with HaloTag
UseTagsHaloTagExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1364 LV EF1a-CD4 IRES-EGFP
Plasmid#201920Purpose2nd generation lentiviral transfer plasmid for engineering cells to express human CD4DepositorInsertCD4 (CD4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
SepRS(2)/pSertRNA(B4)/EF-Sep
Plasmid#173897PurposeThe plasmid contains an orthogonal aminoacyl-tRNA synthetase/tRNACUA pair, which directs the efficient incorporation of phosphoserine (pSer) into recombinant proteins in Escherichia coli.DepositorInsertsSepRS(2)
pSertRNA
EF-Sep
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCfB2904(XI-3 MarkerFree)
Plasmid#73276PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-3 (Chr XI: 93378..94567)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB2909(XII-5 MarkerFree)
Plasmid#73281PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-5 (Chr XII: 839226..840357)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pAL119-TK
Plasmid#21911DepositorInsertHSV Type 1 thymidine kinase
UseAdenoviralTagsExpressionMutationPromoterAvailable sinceOct. 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1345 U6-B2M sgRNA Gag-Cas9 v2
Plasmid#201916PurposeCas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)DepositorInsertsGag-Cas9 v2
B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
UseTagsExpressionMammalianMutationPromoterCAG and Human U6Available sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only