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Plasmid#104583PurposeContains human IgG1 CH1/CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Full Fc-mediated immune effector functionsDepositorInsertHuman IgG1 CH1/CH2/CH3 (IGHG1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pICH41780
Plasmid#48019DepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBS-U6-crRNA-empty
Plasmid#134921PurposeComponents for genome editing in mammalian cells with pPB-CAG-hCas3 and pCAG-All-in-one-hCascade.DepositorInsertcrRNA for CRISPR-Cas3
UseTagsExpressionMammalianMutationPromoterU6Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Leo1
Plasmid#11061DepositorInsertLeo1 (LEO1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pmStrawberry-Atg4BC74A
Plasmid#21076DepositorInsertAtg4B(C74A) (Atg4b Mouse)
UseTagsmStrawberryExpressionMammalianMutation220-222 TGC(Cys) is changed to GCA (Ala)PromoterAvailable sinceJune 8, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-XL4 ASXL1 (p.G646 Wfs *12) 3x FLAG
Plasmid#74245PurposeCancer-associated ASXL1 truncation mutant in mammalian expression vectorDepositorInsertASXL1 (ASXL1 Human)
UseTags3X FLAGExpressionMammalianMutationp.G646 Wfs *12 truncationPromoterCMVAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNAFLAG-ATM-kd
Plasmid#43908DepositorInsertataxia telangiectasia mutated (ATM Human)
UseTagsFLAG and HisExpressionMammalianMutationkinase dead (D2870A and N2875K)PromoterAvailable sinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
Cbh_v5 AAV-CBE C-terminal
Plasmid#137176PurposeAAV genome: expresses the C-terminal of v5 AAV-CBE from the Cbh promoter, and U6-sgRNADepositorInsertv5 AAV-CBE C-terminal; U6-protospacer
UseAAVTagsExpressionMutationPromoterCbhAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBudORF2-CH
Plasmid#51289Purposeexpresses the codon optimized ORF2 of L1RP with his tagDepositorInsertL1 ORF2 (L1RE1 Human)
UseTagsHis and MycExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
FL-BCAN-lentiviral-GFP
Plasmid#29704DepositorInsertBrevican (BCAN Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-synapsin-HaloCaMP1a-EGFP
Plasmid#138327Purposechemigenetic calcium indicatorDepositorHas ServiceAAV1 and AAV9InsertHaloCaMP1a
UseAAVTagsEGFP, His-tag, and Nuclear Exclusion SignalExpressionMammalianMutationPromotersynapsinAvailable sinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
p6sheLLr
Plasmid#37318DepositorInsertHPV6L1+L2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
lentiTet-P2A-mCherry BlastR
Plasmid#186743PurposeDox inducible P2A-mCherry expression construct cloned into a lentiviral backbone containing a blasticidin resistance geneDepositorInsertP2A-mcherry
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUGW-Cytosol-TurboID
Plasmid#200961Purpose3rd gen lentiviral plasmid for expressing cytosol localized TurboID with EGFPDepositorInsertCytosol-TurboID
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHLuc (Antares-SEPLuc C1A4E+6)
Plasmid#141089Purposefinal optimized bioluminescent reporter for detecting extracellular pH changes in tumor acidosisDepositorInsertAntares-T2A-puromycin-IRESv24-SEP-C1A4E+6
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSF463
Plasmid#167671PurposeIntermediate vector for two gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for GatewayDepositorInsertattL1-ccdB-attL2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD_sfGFP3TCG4TAG
Plasmid#174518PurposeRecoded sfGFP reporter with the codons TCG and TAG inserted at positions 3 and 4, respectively.DepositorInsertsfGFP
UseTags6 x HisExpressionBacterialMutationRecoded. TCG and TAG codons were introduced in po…PromoteraraBADAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ECFPEYFP
Plasmid#24520DepositorInsertECFP, EYFP
UseTagsECFP and EYFPExpressionMammalianMutationPromoterAvailable sinceJune 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
GFP scFv [N86/38]
Plasmid#182095PurposeMammalian Expression of GFP scFv. Derived from hybridoma N86/38.DepositorInsertrecombinant mouse scFv targeting GFP
UseTagsHA, HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDV21_feedback_entry_TagBFP-2A-MCS-2A-NLuc-2A-MCP-ADAR2dd(E488Q)-NES
Plasmid#200403PurposeEntry vector for closed-loop ADAR-based RNA sensors (DART VADAR). Expresses TagBFP constitutively. Expresses NanoLuc and MCP-ADAR upon target detectionDepositorInsertClosed-loop DART VADAR sensor entry cassette
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZW1-snoVector
Plasmid#73174PurposeNuclear expression of RNAsDepositorInsertsUseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132B2.0
Plasmid#99888PurposeGolden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAtU3Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-s-IRESWT-Gbetagamma ONE-GO
Plasmid#204340PurposeGPCR/G protein BRET biosensorDepositorInsertGalpha-s/Gbetagamma ONE-GO
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
CIB-VP64 (B1016)
Plasmid#92037PurposeExpresses CIB1 (Full-length, with endogenous NLS) fused to VP64 activation domain (4xVP16 inserts)DepositorInsertCIB1-VP64 (CIB1 Mustard Weed)
UseTagsFusion of plant CIB1 with VP64 activation domain.…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEJS2422_Nme2.Smu-ABE8e-i1
Plasmid#201520PurposeMammalian expression of domain inlaid Nme2.Smu-ABE8e-i1DepositorInsertNme2.Smu-ABE8e-i1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSET-6xTR-TUBE
Plasmid#110313PurposeE. coli expression and purification of 6xTR-TUBEDepositorInsert6xTR-TUBE (UBQLN1 Human)
UseTagsN-terminal His6-T7 tagExpressionBacterialMutationAll Arg residues in the UBA domain are mutated to…PromoterT7Available sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSGV Mouse Acceptor
Plasmid#128176PurposeA TCR Variable Region cloning expression vector using Mouse Constant Regions to reduce mis-pairing with endogenous TCR ref Biotechniques. 2015 Mar 1;58(3):135-9DepositorTypeEmpty backboneUseRetroviralTagsExpressionMutationPromoterMSCVAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO V5 HSPA1A
Plasmid#19510DepositorInsertheat shock 70kDa protein 1A (HSPA1A Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSFFV-EGFP-IRES2-mCherry (FACS reporter)
Plasmid#222999PurposeFACS reporter constitutively expressing mCherry and conditionally expressing EGFP upon prime editing. For lentivirus packaging.DepositorInsertEGFP-IRES2-mCherry
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag beta-1-adrenergic-receptor
Plasmid#14698DepositorInsertB1AR (ADRB1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJune 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
STING-V1
Plasmid#124262PurposeC-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartmentsDepositorInsertTMEM173 (STING1 Human)
UseTagsVenus1ExpressionMammalianMutationPromoterAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK V5-LUC Puro (w543-1)
Plasmid#19360Purpose3rd gen lentiviral V5-Luciferase expression vector, PGK promoter, PuroDepositorInsertFirefly Luciferase
UseLentiviral and LuciferaseTagsV5ExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GGamma2-GFP2
Plasmid#166774PurposeEncodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGGamma2-GFP2 (GNG2 Human, Synthetic)
UseTagsExpressionMammalianMutationContains an N-terminal GFP2 and GSAG linkerPromoterCMVAvailable sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
mito-gTEMP_pcDNA3
Plasmid#109117PurposeMitochondria targetting gTEMPDepositorInsertmito-gTEMP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pKIKOarsBCm
Plasmid#46763PurposeE.coli genomic integration at arsBDepositorTypeEmpty backboneUseE.coli genomic integrationTagsExpressionMutationPromoterAvailable sinceSept. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSL1777 (pEffector)
Plasmid#160731PurposeEffector expression for V. cholerae CAST system, encodes all protein and RNA components. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pCDF backbone.DepositorInsertVchCAST proteins and crRNA
UseTagsExpressionBacterialMutationPromoterJ23119Available sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
NPY-pHTomato
Plasmid#83501PurposeExpression of a fluorescent granule marker, neuropeptide-Y, for localization and exocytosis studies.DepositorInsertNPY (NPY Human)
UseTagspHTomatoExpressionMammalianMutationnonePromoterCMVAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
EK0303 SFFV-mCherry-SG(Bdnf.s3)SG-EGFP (FLP-IN)
Plasmid#191180PurposeNegative control sensor in human cells.DepositorInsertmCherry:Bdnf.s3:EGFP (negative control)
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
3XHalo_GFP_TRF1#1
Plasmid#164644Purposetag TRF1 with GFP and HaloDepositorInsertTRF1 (TERF1 Human)
UseCre/LoxTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only