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Showing: 3721 - 3740 of 4749 results

  2. pJW1584

    Plasmid
    #121055
    Purpose
    YPET^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    YPET^SEC^AID*::3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xFLAG, C. elegans codon optimized YPET, and auxi…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    June 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    121055-plasmid-map-sequence-id-235991

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  3. pFastBac-GFP-dynein-2(D1091–Q4307)

    Plasmid
    #64064
    Purpose
    Codon-optimized human dynein-2 motor domain (D1091–Q4307) for baculovirus expression. 6His-ZZ-GFP N-terminal tag, TEV site to cleave 6His-ZZ.
    Depositor
    Andrew Carter
    Article
    Schmidt et al Nature. 2015 Feb 19;518(7539):435-8. doi
    Insert
    Cytoplasmic dynein-2 (DYNC2H1 Human)
    Use
    Tags
    GFP, His tag, and ZZ tag
    Expression
    Insect
    Mutation
    Deleted amino acids 1-1090, extra C-terminal vali…
    Promoter
    Polyhedrin
    Available since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    64064-plasmid-map-sequence-id-110915

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  4. pETM33_Nsp3d_Ub2_PLPro

    Plasmid
    #156472
    Purpose
    Bacterial expression of Sars-CoV2 Nsp3d_Ub2_PLPro protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp3d_Ub2_PLPro (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156472-plasmid-map-sequence-id-307744

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  6. lentiCRISPR - EGFP sgRNA 3

    Plasmid
    #51762
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 3
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EFS and U6
    Available since
    March 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    51762-plasmid-map-sequence-id-79380

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  9. pcDNA3-SARS-CoV-2-S-RBD-8his-N501Y

    Plasmid
    #164845
    Purpose
    Mammalian expression plasmid for RBD of SARS-CoV-2 (N501Y mutant) with 8his tag
    Depositor
    Erik Procko
    Article
    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf
    Insert
    Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 (S SARS coronavirus 2)
    Use
    Tags
    8his tag and HA leader sequence
    Expression
    Mammalian
    Mutation
    Codon-optimized for human cell expression. Has N5…
    Promoter
    CMV
    Available since
    Jan. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    164845-plasmid-map-sequence-id-322928

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  13. pGLOW39

    Plasmid
    #173068
    Purpose
    mScarlet^SEC^3xMyc vector with ccdB sites for cloning homology arms
    Depositor
    Ryan Doonan
    Article
    Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791
    Insert
    mScarlet-I-C1^SEC^3xMyc
    Use
    CRISPR and Cre/Lox
    Tags
    3xMyc and C. elegans codon-optimized mScarlet
    Expression
    Worm
    Mutation
    Promoter
    Available since
    Oct. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    173068-plasmid-map-sequence-id-345944

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  17. pETM33_Nsp1

    Plasmid
    #156465
    Purpose
    Bacterial expression of Sars-CoV2 Nsp1 protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp1 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156465-plasmid-map-sequence-id-307729

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Showing: 3721 - 3740 of 4749 results