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pMA5096

Plasmid

#184850

Purpose

General purpose retroviral cloning vector, hygromycin resistance

Depositor

Mikhail Alexeyev

Article

Kozhukhar et al Cells. 2022 Jul 11;11(14). pii: cells111

Type

Empty backbone

Use

Cre/Lox and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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SpPLK1-GFP

Plasmid

#185573

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Article

Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

Insert

PLK1

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Expression

Mutation

Promoter

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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SpPLK1L-GFP

Plasmid

#185574

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Article

Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

Insert

PLK1L

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Expression

Mutation

Promoter

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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T7-Phi6-P2

Plasmid

#182535

Purpose

Template for production of Phi6 P2 RNA

Depositor

Peter Sarin

Article

Gregorova et al MethodsX. 2022 May 23;9:101737. doi: 10.

Insert

P2 pol

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pnYC-NvGB1_Y

Plasmid

#148022

Purpose

Bacterial Expression vector

Depositor

Elisa Izaurralde

Article

Diebold et al J Struct Biol. 2011 Aug;175(2):178-88. d

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pH6HTC_mdHUR-Halo

Plasmid

#175312

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

HUR

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pEMF035

Plasmid

#157632

Purpose

sMHT-67

Depositor

Jonathan Silberg

Article

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Inserts

sMHT(1-67)-SYNZIP17
SYNZIP18-sMHT(68-230)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pGBW-m4133156

Plasmid

#152316

Purpose

Mammalian expression plasmid for SARS-CoV-2 nsp8

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp8 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;S-tag

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

Sept. 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4134535

Plasmid

#152312

Purpose

Mammalian expression plasmid for SARS-CoV-2 M (membrane)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;V5

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

Sept. 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4134411

Plasmid

#152311

Purpose

Mammalian expression plasmid for SARS-CoV-2 nsp2

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp2 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;TapTag

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

Sept. 28, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4134519

Plasmid

#152308

Purpose

Mammalian expression plasmid for SARS-CoV-2 nsp2

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp2 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;TRX

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

Sept. 28, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4134408

Plasmid

#152305

Purpose

Mammalian expression plasmid for SARS-CoV-2 nsp9

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp9 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;HA

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

Sept. 28, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4133148

Plasmid

#152304

Purpose

Mammalian expression plasmid for SARS-CoV-2 nsp10

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp10 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;S-tag

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

Sept. 28, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4134416

Plasmid

#152301

Purpose

Mammalian expression plasmid for SARS-CoV-2 nsp2

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp2 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;CBP

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

Sept. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4133932

Plasmid

#152300

Purpose

Mammalian expression plasmid for SARS-CoV-2 nsp6

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp6 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;S-tag

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

Sept. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4133337

Plasmid

#152299

Purpose

Mammalian expression plasmid for SARS-CoV-2 ORF8 protein

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 ORF8 protein (ORF8 Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;S-tag

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

Sept. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4133138

Plasmid

#152297

Purpose

Mammalian expression plasmid for SARS-CoV-2 nsp8

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp8 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;CBP

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

Sept. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4133323

Plasmid

#152295

Purpose

Mammalian expression plasmid for SARS-CoV-2 ORF8 protein

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 ORF8 protein (ORF8 Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;HA

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

Sept. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4134226

Plasmid

#152294

Purpose

Mammalian expression plasmid for SARS-CoV-2 nsp10

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp10 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;V5

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

Sept. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGBW-m4134436

Plasmid

#152293

Purpose

Mammalian expression plasmid for SARS-CoV-2 nsp2

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp2 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;TRX

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

Sept. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge

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