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Plasmid#198671PurposeAn Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiaeDepositorInsertX4_UP
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEM.H03R
Plasmid#198670PurposeAn Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiaeDepositorInsertXII2_DW
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEM.H02R
Plasmid#198668PurposeAn Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiaeDepositorInsertXI2_DW
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEM.H02L
Plasmid#198667PurposeAn Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiaeDepositorInsertXI2_UP
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEM.H01L
Plasmid#198665PurposeAn Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiaeDepositorInsertX3_UP
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pC-HYG-YR
Plasmid#61765PurposeYeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing hygromycin selectable marker on transfer DNA (TDNA).DepositorInsertsHygromycin
2 micron origin or replication and URA3 gene for S. cerevisiae
UseTernary vector for agrobacterium mediated transfo…TagsExpressionMutationPromotertrpC promoterAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCosSAD1AprR
Plasmid#190786PurposeAn E. coli cloning vector for the integration of heterologous genes into a linear plasmid pSA3239 in Streptomyces lavendulae subsp. lavendulae CCM 3239 through homologous recombinationDepositorInsertsupstream region of the aur1 cluster for auricin
ampramycin resistance cassette
downstream region of the aur1 cluster for auricin
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRiboI-APEX2m
Plasmid#176843PurposeExpression of APEX2 from a codon-optimized gene (single-copy integrating plasmid)DepositorInsertpRibo-APEX2m
UseIntegratingTagsExpressionBacterialMutationPromoterpRibo, theophilline riboswitch inducible hsp60 pr…Available sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRibo-APEX2m
Plasmid#176842PurposeExpression of APEX2 from a codon-optimized gene (multi-copy episomal plasmid)DepositorInsertpRibo-APEX2m
UseTagsExpressionBacterialMutationPromoterpRibo, theophilline riboswitch inducible hsp60 pr…Available sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-FRB-W2027F
Plasmid#73341PurposePoint mutation Trp 2027 → Phe in the FKBP12-rapamycin binding (FRB) domain of mTORDepositorInsertFKBP12-rapamycin binding (FRB) domain of MTOR (MTOR Human)
UseTagsGST-taggedExpressionBacterialMutationFRB domain only aa2015-2114; W2027FPromoterAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T FRB Y2105A
Plasmid#68726PurposePoint mutation Tyr 2105 → Ala in the FKBP12-rapamycin binding (FRB) domain of mTORDepositorInsertFKBP12-rapamycin binding (FRB) domain of MTOR (MTOR Human)
UseTagsGST-taggedExpressionBacterialMutationFRB domain only aa2015-2114; Y2105APromoterAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYAMTr2GCsgSc-SeLEU2
Plasmid#224871PurposeDisruption of LEU2 genes in the Saccharomyces sense strict group speciesDepositorUseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF0238
Plasmid#142490PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMYCL (MYCL Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0239
Plasmid#142385PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMYCL (MYCL Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMV306DIhsp+LuxG13
Plasmid#49999PurposeIntegrase removed to increase stability of the plasmid in mycobacteriaDepositorInsertBacterial luciferase operon + G13 promoter
UseTagsExpressionBacterialMutationIt contains a gram-positive enhanced translation …PromoterG13 from M. marinum G13 promoter driving luxCAvailable sinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pMADM-alpha
Plasmid#36890DepositorInsertsGFP
tdTomato-3Myc
beta-globin intron
Neo
ATG (T)
GFP
UseTags3 Myc tagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, deleted…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pKdH_P-GAP-ScACS1*
Plasmid#126736Purposeyeast plasmid overexpressing ACS1 from Saccharomyces cerevisiaeDepositorInsertacs1*
UseTags6xHis,MycExpressionYeastMutationT3542C and A30S, P113S, and S629N (please see dep…PromoterGAPAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKdH_P-GAP-ScADH2+P-GAP*-ScACS1*
Plasmid#126738Purposeyeast plasmid overexpressing ADH2 and ACS1 from Saccharomyces cerevisiaeDepositorInsertadh2,acs1*
UseTags6xHis,MycExpressionYeastMutationT3542CPromoterGAPAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only