Addgene

We narrowed to 12,138 results for: EGFP

Showing: 3721 - 3740 of 12138 results

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pEGFP-WRAP53beta-DC-mutant-R398W

Plasmid

#64683

Purpose

expresses single mutated WRAP53beta at R398W, found in Dyskeratosis congenita patients

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

WD40-encoding RNA antisense to P53 (WRAP53 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

mutation at R398W

Promoter

CMV

Available since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
EB3-eGFP-2xC2-iLID

Plasmid

#114419

Purpose

anchor iLID domain to plasma membrane with slower diffusion

Depositor

Dorus Gadella

Article

Van Geel et al Small GTPases. 2018 Sep 5:1-10. doi: 10.

Insert

iLID domain

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pTox-EGFPsite1_NGA-PAM (BPK754)

Plasmid

#181747

Purpose

p11-lacY-wtx1 derivative toxic plasmid encoding an SpCas9 target site for EGFP site 1 with an NGA PAM

Depositor

Benjamin Kleinstiver

Article

Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

Insert

ccdB toxin gene and SpCas9 target site (EGFP site 1 NGA PAM)

Use

Selection plasmid

Tags

Expression

Mutation

Promoter

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-AaCas12b(R911A)-2AeGFP

Plasmid

#121961

Purpose

Mammalian expression, Transcription regulation, deactivated AaCas12b

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

Catalytically deactivated AaCas12b

Use

Tags

3xHA tag

Expression

Mammalian

Mutation

R911A

Promoter

Available since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pMXs-IP-EGFP-ULK1(1-828)

Plasmid

#38198

Depositor

Noboru Mizushima

Article

Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

Insert

a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 829-1051

Promoter

Available since

Sept. 24, 2012

Availability

Academic Institutions and Nonprofits only
pENTR/pTER shEGFP #1 (628-2)

Plasmid

#17470

Purpose

Gateway entry vector for EGFPshRNA#1 under H1/TO promoter

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Enhanced green fluorescent protein shRNA #1

Use

RNAi; Entry vector

Tags

Expression

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pBabe bleo IRES-EGFP

Plasmid

#14432

Depositor

L. Miguel Martins

Insert

IRES-EGFP

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

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pci-EGFP-UPF1-DEAA

Plasmid

#135996

Purpose

DEAA UPF1 inserted with GFP tagged on the N-terminus used to stably integrate into cells

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

UPF1

Use

Tags

EGFP

Expression

Mammalian

Mutation

DE647AA

Promoter

Available since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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PasCas13b-gRNA-EGFP(W58stop)

Plasmid

#180507

Purpose

PasCas13b-gRNA targeting EGFP(W58stop) for RNA editing

Depositor

Masaharu Somiya

Article

Somiya et al bioRxiv 2022.01.25.477620

Insert

PasCas13b-gRNA targeting EGFP(W58stop)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pci-EGFP-UPF1-R615A

Plasmid

#135995

Purpose

R615A UPF1 inserted with GFP tagged on the N-terminus used to stably integrate into cells

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

UPF1

Use

Tags

EGFP

Expression

Mammalian

Mutation

R615A

Promoter

Available since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-WRAP53beta-DC-mutant-F164L

Plasmid

#64681

Purpose

expresses single mutated WRAP53beta at F164L, found in Dyskeratosis congenita patients

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

WD40-encoding RNA antisense to P53 (WRAP53 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

mutation at F164L

Promoter

CMV

Available since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP-WRAP53beta-DC-mutant-H376Y

Plasmid

#64682

Purpose

expresses single mutated WRAP53beta at H376Y, found in Dyskeratosis congenita patients

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

WD40-encoding RNA antisense to P53 (WRAP53 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

mutation at H376Y

Promoter

CMV

Available since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
GFP-Clavesin1 (pEGFP-C1-Sec14a)

Plasmid

#47421

Depositor

Peter McPherson

Article

Katoh et al J Biol Chem. 2009 Oct 2;284(40):27646-54

Insert

Clavesin1 (CLVS1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
pEGFP-RNF138-delta 86-105

Plasmid

#78922

Purpose

Mammalian expression of RNF138 with delta 86-105 and an EGFP fusion

Depositor

Michael Hendzel

Article

Ismail et al Nat Cell Biol. 2015 Nov;17(11):1446-57.

Insert

RNF138 (RNF138 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

delta 86-105

Promoter

CMV

Available since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
pEGFP-YOD1-MutS2-C160S

Plasmid

#85668

Purpose

Expression of human YOD1 I292Q V295Q C160S mutant with N-terminal GFP tag

Depositor

Hemmo Meyer

Article

Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

Insert

YOD1 (YOD1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

C160S and I292Q and V295Q

Promoter

CMV

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW yoeGFP-T2A-mCherry

Plasmid

#118446

Purpose

Produces equimolar expression of yeast codon-optimized eGFP and mCherry

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

yoeGFP-T2A-mCherry

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-RIα(D/D+linker)-EGFP

Plasmid

#181842

Purpose

EGFP-tagged docking/dimerization (D/D) and linker region of PKA RIα; for mammalian expression.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

RIα-(D/D+linker)EGFP (PRKAR1A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Contains only residues 12-112 of PKA RIα.

Promoter

CMV

Available since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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Nano-lantern-Actin/pEGFP-C1

Plasmid

#51972

Purpose

mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, fused to actin

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern

Use

Tags

Actin

Expression

Mammalian

Mutation

S257G in Rluc8

Promoter

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
Venus-30aa-Noxa-pEGFP-C1

Plasmid

#166762

Purpose

To increase the length of the linker between Venus and Noxa proteins

Depositor

David Andrews

Insert

Noxa (PMAIP1 Human)

Use

Tags

Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmDCP2-V5His6

Plasmid

#22410

Depositor

Elisa Izaurralde

Article

Eulalio et al Mol Cell Biol. 2007 Jun . 27(11):3970-81

Insert

decapping protein 2, variant A (DCP2 Fly)

Use

Drosophila expression

Tags

EGFP and V5-His6

Expression

Mutation

Promoter

Available since

Nov. 5, 2009

Availability

Academic Institutions and Nonprofits only

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