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Plasmid#39821DepositorInsertErk activity reporter
UseTagsHis-MycExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
EKAR2G_design1_mTFP_wt_Venus_wt
Plasmid#39813DepositorInsertErk activity reporter
UseTagsHis-MycExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_GART
Plasmid#183296PurposeAll-in-One CRISPRko system with a guide RNA that targets GART geneDepositorInsertGART
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMK407 (BSD-P2A-mAID-Nluc)
Plasmid#121197PurposeN-terminal taggingDepositorInsertBSD-P2A-mAID-Nluc
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cenp-B DBD INCENP mCherry
Plasmid#45233PurposeTarget INCENP to centromeres via CENP-B DNA binding domain (DBD)DepositorInsertCenp-B-DBD INCENP-Δcen mCherry
UseTagsDNA binding domain of CENP-B and mCherryExpressionMammalianMutationCEN box deleted from INCENP (aa1-46)PromoterAvailable sinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
CpcB-Solanum-lycopersicumPHLS_CpcA
Plasmid#74003PurposeExpresses the Solanum lycopersicum PHLS as a fusion with the CpcB protein plus the NPPS and CmR cassete in SynechocystisDepositorInsertsPHLS and CmR
Neryl diphosphate synthase from Solanum lycopersicum
UseTagsExpressionMutationPromotercpcAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
actb2-lifeact-mKate2
Plasmid#109750PurposeUbiquitous zebrafish expression of mKate2 fused to the actin binding lifeact sequenceDepositorInsertmKate2 fused to the actin binding lifeact sequence
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
sm-9xGCN4-Renilla-MS2
Plasmid#178009PurposeExpresses Spaghetti Monster (sm) 9xGCN4-Renilla mRNA reporter in mammalian cellsDepositorInsertsm-9xGCN4
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
EKAR2G_design1_mTFP_227_Venus_173
Plasmid#39835DepositorInsertErk activity reporter
UseTagsHis-MycExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-sgRNA-com-BoxB-vector
Plasmid#132556PurposeExpresses dCas9-DMNT3a-DMNT3l fusion protein, lambda N22-KRAB fusion protein, and sgRNA scaffold with com and BoxB replacing the Tetraloop and the loop 2 respectively.DepositorInsertsdCas9-DMNT3a-DMNT3l (DNMT3A Synthetic)
Lambda N22-KRAB
sgRNA acaffold with com and BoxB replacing the Tetraloop and the loop 2.
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHJ3: LexA-VHH-Control
Plasmid#108241PurposeLexA DNA binding domain fusing with anti-RNase A VHH antibodyDepositorInsertLexA DNA binding domain fusing with anti-RNase A VHH antibody
UseSynthetic BiologyTagsExpressionMutationPromoterpLacO1Available sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHPK1212
Plasmid#8834DepositorInserttobacco vein mottling virus protease, catalytic domain
UseTagsMBPExpressionBacterialMutationPromoterAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_FGFR1
Plasmid#183289PurposeAll-in-One CRISPRko system with a guide RNA that targets FGFR1 geneDepositorInsertFGFR1
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_FGFR2
Plasmid#183290PurposeAll-in-One CRISPRko system with a guide RNA that targets FGFR2 geneDepositorInsertFGFR2
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-puro-5A, 6A mMCL1-OM
Plasmid#45818DepositorInsert5A, 6A mMCL-1-OM
UseTagsExpressionMammalianMutationChanged Arginine to Alanine at 5th and 6th amino …PromoterAvailable sinceJuly 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_EGFR
Plasmid#183285PurposeAll-in-One CRISPRko system with a guide RNA that targets EGFR geneDepositorInsertEGFR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
EKAR2G_design1_mTFP_wt_Venus_157
Plasmid#39814DepositorInsertErk activity reporter
UseTagsHis-MycExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_SRC
Plasmid#183322PurposeAll-in-One CRISPRko system with a guide RNA that targets SRC geneDepositorInsertSRC
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_ABL2
Plasmid#183268PurposeAll-in-One CRISPRko system with a guide RNA that targets ABL2 geneDepositorInsertABL2
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_ABL1
Plasmid#183267PurposeAll-in-One CRISPRko system with a guide RNA that targets ABL1 geneDepositorInsertABL1
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19/CF-mChe
Plasmid#183166PurposeConstruct for transient expression of cytoplasmic foci-targeted mCherry in plants.DepositorInsertCF-mCherry
UseTagsAtTZF1, A. thaliana TANDEM ZINC FINGER PROTEIN 1ExpressionPlantMutationPromoterAvailable sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19/Tp-mChe
Plasmid#183165PurposeConstruct for transient expression of tonoplast-targeted mCherry in plants.DepositorInsertTp-mCherry
UseTags28 aa of A. thaliana CALCINEURIN B-LIKE PROTEIN 2…ExpressionPlantMutationPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti X2 Puro/pTER shLUC (w589-1)
Plasmid#21472Purpose3rd gen lentiviral Luciferase shRNA (H1/TO promoter), 3’LTR insertion, PuroDepositorInsertFirefly luciferase shRNA
UseLentiviral and RNAiTagsExpressionMutationPromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pHU6_chr12_FAM19A2-down-gRNA-for
Plasmid#81223PurposepHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the downstream region of FAM19A2 locusDepositorInserthU6 expression of gRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHU6_chr12_FAM19A2-up-gRNA-rev
Plasmid#81220PurposepHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the upstream region of FAM19A2 locusDepositorInserthU6 expression of gRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHU6_chr12_FAM19A2-up-gRNA-for
Plasmid#81221PurposepHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the upstream region of FAM19A2 locusDepositorInserthU6 expression of gRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHU6_chr12_FAM19A2-down-gRNA-rev
Plasmid#81222PurposepHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the downstream region of FAM19A2 locusDepositorInserthU6 expression of gRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_HDAC3
Plasmid#183299PurposeAll-in-One CRISPRko system with a guide RNA that targets HDAC3 geneDepositorInsertHDAC3
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_ESR1
Plasmid#183288PurposeAll-in-One CRISPRko system with a guide RNA that targets ESR1 geneDepositorInsertESR1
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_DNMT1
Plasmid#183282PurposeAll-in-One CRISPRko system with a guide RNA that targets DNMT1 geneDepositorInsertDNMT1
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_AAVS1B
Plasmid#183338PurposeAll-in-One CRISPRko system with a guide RNA that targets the AAVS1 locusDepositorInsertAAVS1B
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
EKAR2G_design2_mTFP_105_Venus_195
Plasmid#40002DepositorInsertErk activity reporter
UseTagsHis-MycExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFSW Syt1-C2B IRES GFP
Plasmid#133817PurposeEncodes the C2B domain of synaptotagmin 1 for viral expressionDepositorInsertSyt1 (Syt1 Rat)
UseLentiviralTagsIRES-GFPExpressionMutationPromoterhSyn (human synapsin I promoter)Available sinceJuly 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
Avr2-GFP pZK538
Plasmid#118011Purposeexpresses Avr2-GFP (without signal peptide) and mCherry-HDEL. Used for visualization of cell-to-cell movement of Avr2. Avr2 can be replaced with gene of interest via HiFi DNA assembly cloning (NEB) .DepositorArticleInsertsdspAvr2
mCherry
UseTagsEGFP and HDELExpressionBacterial and PlantMutationPromoter35SAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-λN-βLac
Plasmid#162039PurposeExpresion of the fusion protein of the λN domain of the λ phage and ampR gene (encoding the enzyme β-lactamase without the native signal sequence)DepositorInsertλN domain of the λ phage and a β-lactamase fusion protein
UseTags6xHis tagExpressionBacterialMutationPromoterT7Available sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNB655
Plasmid#60142PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by MET17 promoter.DepositorInsertFLuc-yEVenus
UseTagsExpressionYeastMutationA4VPromoterMET17prAvailable sinceFeb. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCSDest2-SpCas9-NLS-3XHA-NLS
Plasmid#69220PurposeExpresses wild type SpCas9 in mammalian cellsDepositorInsertSpCas9
UseCRISPRTagsNLS-3XHA-NLSExpressionMammalianMutationPromoterAvailable sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFUPI
Plasmid#80279PurposeA bicistronic lentiviral vector used to stably express cDNAs.DepositorInsertPuro resistance cassette, IRES, cloning site
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti X2 Neo/pTER shLUC (w181-1)
Plasmid#17483Purpose3rd gen lentiviral Luciferase shRNA (H1/TO promoter), 3’LTR insertion, NeoDepositorInsertFirefly Luciferase shRNA
UseLentiviral and RNAiTagsExpressionMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
4E2H.210_LUMABS
Plasmid#155209PurposeExpresses 4E2H.210_LUMABS sensor in E.coliDepositorInsert4E2H.210_LUMABS
UseTags6x His TagExpressionMutationPromoterAvailable sinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only