-
Plasmid#179042PurposeVector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-YPT7, a marker for the vacuole, late endosomeDepositorInsertRPL18Bp-Neon(gfp)-Link-YPT7
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK57 pHomL-RPL18Bp-SNF7-Link-Neon(gfp)-SSA1t-HomR (AmpR)
Plasmid#179041PurposeVector containing a yeast integration cassette for RPL18B-driven expression of SNF7-Link-Neon(gfp), a marker for the endosome, class E compartmentDepositorInsertRPL18Bp-SNF7-Link-Neon(gfp)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK56 pHomL-RPL18Bp-Neon(gfp)-Link-VPS21-SSA1t-HomR (AmpR)
Plasmid#179040PurposeVector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-VPS21, a marker for the endosomesDepositorInsertRPL18Bp-Neon(gfp)-Link-VPS21
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFendoNA2S
Plasmid#174730PurposeExpresses GFP-endoNA2-S706A fusion protein for detection of polysialic acid. The endoNA2-S706A is a noncatalytic endosialidase that lacks the ability for releasing its C-terminal chaperone domain.DepositorInsertendoNA2-S706A
UseTagsExpressionBacterialMutationChanged Serine 706 to AlaninePromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
D74_Ld_291730
Plasmid#135887PurposeExpression of Leishmania donovani ectodomain in mammalian cellsDepositorInsertendosomal integral membrane protein, putative
UseTagsbiotinylation sequence and His tagExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Akt2(K14A/R25E)-Halo-PS
Plasmid#86632PurposeExpresses eGFP-Akt2-HaloTag-PS fusion (cytosolic tandem)DepositorInsertAkt2(K14A/R25E)-Halo-PS (AKT2 )
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-RAB7A-IRES-Puromycin
Plasmid#133027PurposeExpresses EGFP-tagged late endosome markerDepositorInsertRAB7A (RAB7A Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterHuman elongation factor 1 alphaAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB713B-pCMV-MITD-NeoMini-T2A-tCD19-WPRE
Plasmid#174610PurposePiggyBack transposon expressing a fusion of a tandem minigene antigens in KRAS, BRAF and CMV fused to an endosomal targeting sequence and translationally linked to human truncated CD19DepositorInserthuman CD19, antigen minigene
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pB80-KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)_P2A_iLID-mCherry-RAB11
Plasmid#174644PurposeOptogenetic coupling of RAB11 to opto-kinesin to induce anterograde transport of recycling endosomesDepositorInsertKIF1A(1-365)-VVDfast-mVenus-SSPB(micro)-P2A-iLID-mCherry-RAB11
UseTagsVenus-SSPB and iLID-mCherryExpressionMammalianMutationmmKIF1A(aa1-365): Pro202Ala; mVenus: Met1Del, Thr…PromoterChicken beta-actinAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSCV-human CSF-1R-Y969F,S301-IRES—GFP
Plasmid#115524PurposeMammalian expression constructDepositorInserthuman CSF-1 receptor constitutively activated mutant (CSF1R Human)
UseRetroviralTagsExpressionMutationY969F,S301PromoterAvailable sinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV-human CSFI-R-L301S- Y969F-IRES-GFP
Plasmid#133745Purposeretroviral expression of CSF1-R mutantDepositorInsertCSF1-R (CSF1R Human)
UseRetroviralTagsExpressionMammalianMutationL301S- Y969FPromoterMSCVAvailable sinceDec. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTag-RFP-C-h-Rab4a-c-Myc
Plasmid#79800PurposeExpress TagRFP labeled human Rab5a (GTPase located on recycling endosomal membranes).DepositorInsertRab4a (RAB4A Human)
UseTagsTagRFP and mycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCXLE-hK + E2-Crimson
Plasmid#74946PurposeDosage control and tracing of reprogramming episomesDepositorInsertKlf4 (KLF4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTag-BFP-C-h-Rab4a-c-Myc
Plasmid#79799PurposeExpress TagBFP labeled human Rab4a (GTPase located on recycling endosomal membranes).DepositorInsertRab4a (RAB4A Human)
UseTagsTagBFP and mycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
actc1b-mKate2-EEA1
Plasmid#109508PurposeMuscle specific zebrafish expression of mKate2 fused to early endosome associated 1DepositorInsertEEA1 (eea1 Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMCSF-R(m40)-luc
Plasmid#12425DepositorInsertM-CSF receptor promoter fragment (CSF1R Human)
UseLuciferaseTagsExpressionMutationPU.1 binding site mutation (created a KpnI site i…PromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBS-AMVpl-β1WT-nAchR
Plasmid#97080PurposeTo determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function via dose-response curves from voltage-clamped oocytes to estimate EC50's and Hill CoDepositorInsertBeta1 nicotinic acetylcholine receptor (Chrnb1 Mouse)
UseTagsExpressionBacterialMutationWTPromoterT7Available sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
mIFP12-Endo-14
Plasmid#56250PurposeLocalization: Endosomes, Excitation: 683, Emission: 704DepositorInsertEndo (RHOB Human)
UseTagsMyc and mIFP12ExpressionMammalianMutationDeleted amino acids 1-4PromoterCMVAvailable sinceApril 28, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC19-Q0P897
Plasmid#103120PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertQ0P897(Cas9 coding gene from Rhodospirillum rubrum (strain ATCC 11170 / NCIB 8255))
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBiex PKC - Peptide 14 mCit mCer
Plasmid#83565PurposeExpresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the pseudosubstrate of PKCalpha.DepositorInsertPKC alpha (PRKCA Human)
UseTags10 nm ER/K Helix, FLAG Purificaiton Tag, Tev Prot…ExpressionBacterial and InsectMutationOnly catalytic domain of PKC (aa 335-672)PromoterT7 PromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
PCXLE-hUL + mTAGBFP2
Plasmid#74944PurposeDosage control and tracing of reprogramming episomesDepositorUseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
UbC-blasticidine-P2A-TETon-3G transactivator
Plasmid#200191PurposeA TETon activator, which combined with the DiLiCre 2.0 plasmid, drives the expression of DiLiCre 2.0 upon doxycycline. DiLiCre 2.0 is a cre recombinase that is activated by violet light.DepositorInsertblasticidine - P2A - TETon-3G transactivator
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterUbCAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBullet-end-c
Plasmid#53074Purposedestination vector with ECFP- endosome marker (RabF2a) for tagged fluorescent protein (C-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDTFendoNA2
Plasmid#174731PurposeExpresses DT386F-endoNA2 fusion protein for selective elimination of polysialic acid–containing cells. Truncated diphtheria toxin fused to noncatalytic endosialidase via a furin-cleavable linker.DepositorInsertDT386F
UseTagsExpressionBacterialMutationDeleted amino acids 412-560 (the receptor binding…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5-FRT-TO-SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)_P2A_iLID-mCherry-RAB11
Plasmid#174647PurposeOptogenetic coupling of RAB11 to tetramerized moss kinesin-14 to induce retrograde transport of recycling endosomes. Compatible with Flp-in TREX system.DepositorInsertSSPB(micro)-mVenus-GCN4-ppKin14Vib(816-1321)-P2A-iLID-mCherry-RAB11
UseTagsExpressionMammalianMutationSSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…PromoterCMV (with TetOn)Available sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pB80-SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)_P2A_iLID-mCherry-RAB11
Plasmid#174645PurposeOptogenetic coupling of RAB11 to tetramerized moss kinesin-14 to induce retrograde transport of recycling endosomesDepositorInsertSSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)-P2A-ILID-mCherry-RAB11
UseTagsSSPB(micro)-Venus and iLID-mCherryExpressionMammalianMutationSSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…PromoterChicken beta-actinAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS-AMVpl-δWT-nAchR
Plasmid#97188PurposeTo determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function via dose-response curves from voltage-clamped oocytes to estimate EC50's and Hill CoDepositorInsertDelta nicotinic acetylcholine receptor (Chrnd Mouse)
UseTagsExpressionBacterialMutationWTPromoterT7Available sinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBS-AMVpl-γWT-nAchR
Plasmid#97187PurposeTo determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function via dose-response curves from voltage-clamped oocytes to estimate EC50's and Hill CoDepositorInsertGamma nicotinic acetylcholine receptor (Chrng Mouse)
UseTagsExpressionBacterialMutationWTPromoterT7Available sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBullet-end-n
Plasmid#53073Purposedestination vector with ECFP- endosome marker (RabF2a) for tagged fluorescent protein (N-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5-FRT-TO-KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)_P2A_iLID-mCherry-RAB11
Plasmid#174646PurposeOptogenetic coupling of RAB11 to opto-kinesin to induce anterograde transport of recycling endosomes. Compatible with Flp-in TREX system.DepositorInsertKIF1A(1-365)-VVDfast-mVenus-SSPB(micro)-P2A-iLID-mCherry-RAB11
UseTagsExpressionMammalianMutationmmKIF1A(aa1-365): Pro202Ala; mVenus: Met1Del, Thr…PromoterCMV (with TetOn)Available sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDTCendoNA2
Plasmid#174732PurposeExpresses DT386C-endoNA2 fusion protein for selective elimination of polysialic acid–containing cells. Truncated diphtheria toxin fused to noncatalytic endosialidase via a caspase-3/7-cleavable linkerDepositorInsertDT386C
UseTagsExpressionBacterialMutationDeleted amino acids 412-560 (the receptor binding…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
DiLiCre 2.0
Plasmid#198752PurposeA Cre recombinase that is expressed upon doxycycline when combined with the UbC-blasticidine-P2A-TETon-3G transactivator. DiLiCre 2.0 is a cre recombinase that is activated by violet light.DepositorInsertCre-PhoCI-ERT2-ERT2
UseLentiviralTagsCre, kept in a photocleavable site, followed by 2…ExpressionBacterial and MammalianMutationPromoterTRE3GVAvailable sinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMCSF-R(mB)-luc
Plasmid#12422DepositorInsertM-CSF receptor promoter fragment (CSF1R Human)
UseLuciferaseTagsExpressionMutationAML1 binding site mutation (created a KpnI site i…PromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMCSF-R(mA)-luc
Plasmid#12421DepositorInsertM-CSF receptor promoter fragment (CSF1R Human)
UseLuciferaseTagsExpressionMutationC/EBP binding site mutation (created a KpnI site …PromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMCSF-R(DD)-luc
Plasmid#12424DepositorInsertM-CSF receptor promoter fragment (CSF1R Human)
UseLuciferaseTagsExpressionMutationDeletion from bp-86 to -37 (created a KpnI site i…PromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
PB-GFP-Gal8
Plasmid#127191PurposePiggybac transposon plasmid with CAG promoter GFP-Galectin 8 (GFP-Gal8) fusion protein. Useful as genetically encoded endosomal escape sensor.DepositorInsertGFP-Gal8 (LGALS8 Human)
UseTagsGal8 is fused to the c-terminus of GFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CSF1R-V5/HIS
Plasmid#201984Purposeexpression of human CSF1R receptor tyrosine kinase in mammalian cellsDepositorInsertCSF1R receptor tyrosine kinase (CSF1R Human)
UseTagsV5, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EML4-ALK variant 1
Plasmid#183828PurposeExpress EML4-ALK fusion variant 1 (E13;A20) in mammalian cellsDepositorUseLentiviralTagsThe fusion protein of EML4 exon 1-13 and ALK exon…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EML4-ALK variant 3a
Plasmid#183829PurposeExpress EML4-ALK fusion variant 3a (E6a;A20) in mammalian cellsDepositorUseLentiviralTagsThe fusion protein of EML4 exon 1-6a and ALK exon…ExpressionMammalianMutationPromoterCMVAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ABL1
Plasmid#23939DepositorInsertABL1 (ABL1 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only