We narrowed to 27,513 results for: sta

1. pRG01-U6-DR-crRNA-BsmbI(x2)-6T; EFS-Puro-2A-Fluc-WPRE

   Plasmid

   #123362

   Purpose

   Lentiviral vector with empty U6 cassette containing LbCpf1 direct repeat and U6 terminator, with constitutive expression of puromycin resistance and Firefly luciferase.

   Depositor

   Sidi Chen

   Article

   Chow et al Nat Methods. 2019 May;16(5):405-408. doi

   Insert

   Firefly luciferase
   

   Use

   CRISPR, Lentiviral, and Luciferase

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKS021 - pCAGGS-3XFLAG-mKate2-SA1-bpA

   Plasmid

   #156440

   Purpose

   For transient expression of mouse SA1 (= STAG1 or SCC3A) tagged with mKate2

   Depositor

   Elphege Nora

   Article

   Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

   Insert

   mouse SA1 (Stag1 Mouse)
   

   Expression

   Mammalian

   Mutation

   none

   Available Since

   Sept. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pS148∙CsR

   Plasmid

   #197858

   Purpose

   Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

   Depositor

   Susanne Häußler

   Article

   Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

   Insert

   gRNA scaffold - Ap/Cb resistance cassette - incP oriT
   

   Use

   CRISPR and Synthetic Biology

   Promoter

   PEM7

   Available Since

   Oct. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pS548∙CsR

   Plasmid

   #197859

   Purpose

   Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

   Depositor

   Susanne Häußler

   Article

   Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

   Insert

   gRNA scaffold - Tc resistance cassette - incP oriT
   

   Use

   CRISPR and Synthetic Biology

   Promoter

   PEM7

   Available Since

   Oct. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDIRECT_26G

   Plasmid

   #91149

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA , Plant Selection: PvUbi2:bar

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   D10A, H840A

   Available Since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pS848∙CsR

   Plasmid

   #199240

   Purpose

   Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

   Depositor

   Susanne Häußler

   Article

   Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

   Insert

   gRNA scaffold - Gm resistance cassette - incP oriT
   

   Use

   CRISPR and Synthetic Biology

   Promoter

   PEM7

   Available Since

   Oct. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-2E2-HA-mCh

   Plasmid

   #129596

   Purpose

   The encoded protein is the anti-HA frankenbody variant fused with the mCherry. It can be used to track mature and nascent HA tagged proteins in living organism.

   Depositor

   Tim Stasevich

   Article

   Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

   Insert

   Anti-HA frankenbody variant-mCherry (2E2-HA scFv-mCherry)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-2E2-HA-mEGFP

   Plasmid

   #129595

   Purpose

   The encoded protein is the anti-HA frankenbody variant fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.

   Depositor

   Tim Stasevich

   Article

   Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

   Insert

   Anti-HA frankenbody variant-mEGFP (2E2-HA scFv-mEGFP)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKS022 - pCAGGS-3XFLAG-mKate2-SA2-bpA

   Plasmid

   #156441

   Purpose

   For transient expression of mouse SA2 (STAG2 or SCC3B) tagged with mKate2

   Depositor

   Elphege Nora

   Article

   Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

   Insert

   mouse SA2 (Stag2 Mouse)
   

   Expression

   Mammalian

   Mutation

   none

   Available Since

   Sept. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDIRECT_23C

    Plasmid

    #91140

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:bar

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. SF3B3-1

    Plasmid

    #156004

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    SF3B3 (SF3B3 Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMP71-super-IL-2

    Plasmid

    #174599

    Purpose

    Expresses extracellular domain of human IL-2 (aa 21-153) (mutant H9 containing the mutations L80F / R81D / L85V / I 86V / I92F) developed by the Garcia Lab

    Depositor

    Stanley Riddell

    Article

    Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

    Insert

    extracellular domain of huIL-2 (aa 21-153) (mutant H9 containing the mutations L80F / R81D / L85V / I 86V / I92F)
    

    Expression

    Mammalian

    Available Since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV-CAG::FLEX-rev:: ChR2HA-2a-hM4D

    Plasmid

    #52521

    Purpose

    Co-expresses HA tagged channelrhodopsin and hM4D (Gi) from a Cre-dependent virus

    Depositor

    Scott Sternson

    Article

    Stachniak et al Neuron. 2014 May 21;82(4):797-808. doi:

    Insert

    ChR2-2a-hM4D
    

    Use

    AAV and Cre/Lox

    Tags

    2xHA on ChR2

    Promoter

    CBA

    Available Since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1D/mEGFP + CrPV IGR IRES-nLuc-3XFLAG-CL1PEST

    Plasmid

    #127332

    Purpose

    Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CrPV IRES

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Tags

    3xFLAG and CL1/PEST

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. mCherry-N2-Frankenbody-HA

    Plasmid

    #175292

    Purpose

    Visualize endogeneous loci coupled with ZF probes harboring 10xHA tag

    Depositor

    Tim Stasevich

    Article

    Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

    Insert

    anti-HA frankenbody fused with mCherry
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGPTVII-SED1

    Plasmid

    #232615

    Purpose

    SED1 biosensor for expression in Arabidopsis thaliana

    Depositor

    Jose Dinneny

    Article

    Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

    Insert

    SED1 Biosensor
    

    Expression

    Plant

    Promoter

    pUBQ10

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEF5B-FRT-AP-Sstr3-GFP-DEST

    Plasmid

    #49098

    Purpose

    Expressing AP-Sstr3-GFP in mammalian cells, can be used for establishing Flp-In stable cell lines

    Depositor

    Maxence Nachury

    Article

    Ye et al Elife. 2013 Aug 6;2:e00654. doi: 10.7554

    Insert

    Sstr3 (Sstr3 Mouse)
    

    Tags

    GFP and acceptor peptide (AP)

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Nov. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIRES-Puro-MGMT

    Plasmid

    #187654

    Purpose

    Expresses hMGMT together with the puromycin resistance gene via an IRES.

    Depositor

    Robert Sobol

    Article

    Li et al Cancers (Basel). 2022 Jul 22;14(15). pii

    Insert

    MGMT (MGMT Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNAintron-CHIKV-Structural polyprotein

    Plasmid

    #215698

    Purpose

    Produces the Chikungunya virus structural polyprotein Capsid-E3-E2-6K-E1

    Depositor

    Melinda Brindley

    Article

    Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

    Insert

    CHIKV structural polyprotein Capsid-E3-E2-6K-E1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pOTTC840 - pAAV 5xATF6 secNLuc

    Plasmid

    #82497

    Purpose

    Longitudinal monitoring of Gaussia and Nano luciferase activities to simultaneously assess ER calcium homeostasis and ER stress in vivo

    Depositor

    Brandon Harvey

    Article

    Wires et al PLoS One. 2017 Apr 12;12(4):e0175481. do

    Insert

    secNLuc
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    5x(ATF6 binding site) c-Fos minimal promoter

    Available Since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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