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Plasmid#180649PurposeLentiviral expression vector for SPASTIN. Has L177D mutation. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. SPASTIN starts on M87. Internal ID: WISP20-45.DepositorInsertSPASTIN
UseLentiviralTagsmCherryExpressionMammalianMutationStarts at M87, L177D mutation, has siRNA resistan…PromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 - circMAN2A1
Plasmid#198837PurposeThis contains the 400nt isoform of exons two and three from MAN2A1 circular RNA, 3X Flag tag inserted in exon twoDepositorInsertcircular mannosidase alpha class 2A member 1
UseTagsExpressionMammalianMutationcircular RNA composed by 2 exonsPromoterCMVAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX FLAG-SLC38A9.1
Plasmid#199749PurposeLentiviral construct to stably express SLC38A91.1DepositorInsertSLC38A9.1
UseTagsFlagExpressionMammalianMutationPromoterEF1aAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-PAL1D
Plasmid#196686PurposeRep/Cap plasmid for the production of PAL1D, an AAV capsid with CNS tropism in macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVTagsExpressionMutationPTQGTFR insert between amino acids 588 and 589 of…Promoterp41Available sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-M.Fas.1
Plasmid#196687PurposeRep/Cap plasmid for the production of M.Fas.1, an AAV capsid with CNS tropism in macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVTagsExpressionMutationTDALTTK insert between amino acids 588 and 589 of…Promoterp41Available sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-PAL1B
Plasmid#196684PurposeRep/Cap plasmid for the production of PAL1B, an AAV capsid with CNS tropism in macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVTagsExpressionMutationPSQGTLR insert between amino acids 588 and 589 of…Promoterp41Available sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-PAL1A
Plasmid#196683PurposeRep/Cap plasmid for the production of PAL1A, an AAV capsid with CNS tropism in macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVTagsExpressionMutationPTQGTVR insert between amino acids 588 and 589 of…Promoterp41Available sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-M.Fas.4
Plasmid#196690PurposeRep/Cap plasmid for the production of M.Fas.4, an AAV capsid with CNS tropism in macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVTagsExpressionMutationVVSDYTV insert between amino acids 588 and 589 of…Promoterp41Available sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-M.Fas.3
Plasmid#196689PurposeRep/Cap plasmid for the production of M.Fas.3, an AAV capsid with CNS tropism in macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVTagsExpressionMutationRVDPSGL insert between amino acids 588 and 589 of…Promoterp41Available sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-M.Fas.2
Plasmid#196688PurposeRep/Cap plasmid for the production of M.Fas.2, an AAV capsid with CNS tropism in macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVTagsExpressionMutationSTIPTMK insert between amino acids 588 and 589 of…Promoterp41Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
trp1D-proLYS2-GFP-PKCdelta(C1a+C1b)-NAT
Plasmid#184774PurposeMarker for DAG. Yeast expression of mouse PKCdelta under LSY2 promoter. Uses antibiotic resistance marker natMX6. Replaces endogenous TRP1 upon genome integration, leading to trp1D.DepositorInsertproLYS2-GFP-PKCdelta(C1a+C1b) (Prkcd Mouse, Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cas9_nonPuro_OSS
Plasmid#186652PurposeCas9 plasmid to express the Cas9 in OSS cellsDepositorInsertOSS Cas9 Expression Plasmid
UseTagsExpressionInsectMutationPromoterAvailable sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET303C-hSOD1 A4V/C6S
Plasmid#139667PurposeExpression plasmids containing human A4V/C6S SOD1DepositorInsertSuperoxide dismutase-1 (SOD1 Human)
UseTagsExpressionBacterialMutationchange alanine 4 to valine, change cysteine 6 to …PromoterT7Available sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2361_Tier3(SB)-rtTA-YPet_PuroR
Plasmid#169657PurposeTier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a YPet marker and PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)DepositorInsertPPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
UseTagsExpressionMammalianMutationPromoterPRPBSAAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2363_Tier3(PB)-rtTA-YPet_PuroR
Plasmid#169659PurposeTier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a YPet marker and PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)DepositorInsertPPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
UseTagsExpressionMammalianMutationPromoterPRPBSAAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKT2/CAGXSP/PalmGamillus
Plasmid#182971PurposeStably express PalmGamillus in mammalian cells via the sleeping beauty transposon system.DepositorInsertPalmGamillus
UseTagsExpressionMammalianMutationN/APromoterAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGem-LP1-loxP-bar-lox2272-71
Plasmid#168785PurposeVector to create landing pad 1 (LP1) by homologous recombination in Aspergillus nidulans ΔSt site. The strain A. nidulans LP1 is used for recombinase mediated chromosomal integration.DepositorInsertsbar
Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
loxP
lox2272-71
UseCre/Lox and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGem-LP2-loxP-bar-lox2272-71
Plasmid#168786PurposeVector to create landing pad 2 (LP2) by homologous recombination in Aspergillus nidulans IS1 site. The strain A. nidulans LP2 is used for recombinase mediated chromosomal integration.DepositorInsertsbar
IS1 Homology arm 1
IS1 Homology arm 2
loxP
lox2272-71
UseCre/Lox and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-PolB(T304I)-Puro
Plasmid#177143PurposeLentiviral vector expressing Flag-PolB(T304I) and a puromycin resistance cassetteDepositorInsertPolB (POLB Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
Clim Actin MCH Puro (CAMP)
Plasmid#104446PurposeExpresses mCherry under endogenous actin promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope. Puromycin resistant.DepositorInsertmCherry
UseExpression in corallochytrium (single-celled euka…TagsExpressionMutationPromoteractinAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only