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pBZ186-pU6-(BbsI)_CBh-sv40NLS-Cas9-NLS T2A-mCherry

Plasmid

#170182

Purpose

Mammalian expression vector for sgRNAs cloned into the BbsI sites and for expression of spCas9 linked to mCherry via a T2A peptide

Depositor

Hunter Fraser

Article

Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris

Insert

mCherry

Use

Tags

3XFlag and T2A

Expression

Mammalian

Mutation

Promoter

CBh

Availability

Academic Institutions and Nonprofits only

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pBZ191-pU6-sgBFP-CBh-sv40NLS-Cas9-NLS-T2A-mCherry

Plasmid

#170183

Purpose

Expression vector for a sgRNA against BFP and spCas9 linked to mCherry via a T2A peptide

Depositor

Hunter Fraser

Article

Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris

Insert

sgBFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Availability

Academic Institutions and Nonprofits only

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pJ204-loxP-EF1a-mCherry-NeoR-lox2272-BGHpA-siteA-HDR

Plasmid

#154826

Purpose

Landing pad for Cre/lox-based recombinase-mediated cassette exchange (RMCE) in CHO cells, donor plasmid for CRISPR/Cas9-mediated targeted integration in CHO cells (site A)

Depositor

Lise Marie Grav

Article

Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

Inserts

mCherry
NeoR
ZsGreen1-DR

Use

CRISPR and Cre/Lox

Tags

PEST

Expression

Mammalian

Mutation

Promoter

CMV, EF1a, and SV40 early promoter

Availability

Academic Institutions and Nonprofits only

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pHR: pmU6 sgChr3q29 2xMS2 pUbC MCP-mCherry-p2a-Puro

Plasmid

#174118

Purpose

Expression of sgRNA targeting Chr3q29 (chr3: 195478317 - 195506985, hg38)

Depositor

Stanley Qi

Article

Gao et al Mol Cell. 2021 Aug 13. pii: S1097-2765(2

Insert

sgChr3q29 2xMS2 and MCP-mCherry-p2a-Puro

Use

Lentiviral

Tags

Expression

Mutation

Promoter

mU6 and UbC

Availability

Academic Institutions and Nonprofits only

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pHR: pmU6 sgChr13q34 2xMS2 pUbC MCP-mCherry-p2a-Puro

Plasmid

#174119

Purpose

Expression of sgRNA targeting Chr13q34 (chr13: 112277011 - 112319170, hg38)

Depositor

Stanley Qi

Article

Gao et al Mol Cell. 2021 Aug 13. pii: S1097-2765(2

Insert

sgChr13q34 2xMS2 and MCP-mCherry-p2a-Puro

Use

Lentiviral

Tags

Expression

Mutation

Promoter

mU6 and UbC

Availability

Academic Institutions and Nonprofits only

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pMB952:PB-CAG-PuroR-nGFP-FLEx-SaCas9-P2A-mCherry-WPRE

Plasmid

#168107

Purpose

PiggyBac transposon vector constitutively driving PuroR-eGFP and conditionally expressing S.aureus Cas9 and mCherry

Depositor

Erich Jarvis

Article

Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10

Insert

Cas9-P2A-mCherry

Use

CRISPR and Cre/Lox

Tags

HA

Expression

Mutation

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-Rac-WT(guide resistant)-2a-mcherry-U6ac-Rac guides

Plasmid

#168242

Purpose

"Rescue Rac expression in neutrophil specific knockout"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

Rac(guide resistant)-WT

Use

Zebrafish expression

Tags

mcherry

Expression

Mutation

Promoter

LyzC

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-Rac-DN(guide resistant)-2a-mcherry-U6ac-Rac guides

Plasmid

#168243

Purpose

"Dominant negative control of the assay to rescue Rac expression in neutrophil specific knockout"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

Rac(guide resistant)-DN

Use

Zebrafish expression

Tags

mcherry

Expression

Mutation

Promoter

LyzC

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-Rac-CA(guide resistant)-2a-mcherry-U6ac-Rac guides

Plasmid

#168244

Purpose

"Rescue Rac-CA expression in neutrophil specific knockout"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

Rac(guide resistant)-CA

Use

Zebrafish expression

Tags

mcherry

Expression

Mutation

Promoter

LyzC

Availability

Academic Institutions and Nonprofits only

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pRP[2CRISPR]-mCherry/Hygro-hCas9-U6>{mdx left}-U6>{mdx right}

Plasmid

#184381

Purpose

This plasmid contains hCas9 and two gRNAs that can excise the genomic area around the mouse mdx mutation in dystrophin's exon 23

Depositor

Ori Bar-Nur

Article

Domenig et al Stem Cell Reports. 2022 Feb 8;17(2):321-

Insert

Cas9, two gRNAs targeting dystrophin

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pJT050 lenti pEF-rTetR-LibCloneSite-T2A-mCherry-BSD-WPRE

Plasmid

#161925

Purpose

Lentiviral backbone for GoldenGate cloning effectors as a fusion with rTetR

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315

Type

Empty backbone

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

pEF

Availability

Academic Institutions and Nonprofits only

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pSL011_AAVS1-puro-9xTetO-pEF-H2B-mCitrine-pRSV-H2B-mCherry

Plasmid

#179428

Purpose

Dual fluorescent reporter construct with no spacer, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus

Depositor

Lacra Bintu

Article

Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

Insert

TRE-cit-(nospacer)-mch

Use

Tags

Expression

Mammalian

Mutation

Promoter

pEF alpha, pRSV

Availability

Academic Institutions and Nonprofits only

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pMH002_phiC31_neo-5xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry

Plasmid

#179430

Purpose

Dual fluorescent reporter construct with single HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration

Depositor

Lacra Bintu

Article

Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

Insert

TRE-cit-HS4-mch (SH)

Use

Tags

Expression

Mammalian

Mutation

Promoter

pEF alpha, pRSV

Availability

Academic Institutions and Nonprofits only

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pMH003_phiC31_neo-5xTetO-pEF-H2B-mCitrine-coreHS4-pRSV-H2B-mCherry

Plasmid

#179431

Purpose

Dual fluorescent reporter construct with single core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration

Depositor

Lacra Bintu

Article

Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

Insert

TRE-cit-coreHS4-mch (SC)

Use

Tags

Expression

Mammalian

Mutation

Promoter

pEF alpha, pRSV

Availability

Academic Institutions and Nonprofits only

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pMH011_AAVS1_puro-pA-9xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry

Plasmid

#179434

Purpose

Dual fluorescent reporter construct with single HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus

Depositor

Lacra Bintu

Article

Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

Insert

TRE-cit-HS4-mch (SH)

Use

Tags

Expression

Mammalian

Mutation

Promoter

pEF alpha, pRSV

Availability

Academic Institutions and Nonprofits only

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pMH012_AAVS1_puro-pA-9xTetO-pEF-H2B-mCitrine-core-pRSV-H2B-mCherry

Plasmid

#179435

Purpose

Dual fluorescent reporter construct with single core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus

Depositor

Lacra Bintu

Article

Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

Insert

TRE-cit-coreHS4-mch (SC)

Use

Tags

Expression

Mammalian

Mutation

Promoter

pEF alpha, pRSV

Availability

Academic Institutions and Nonprofits only

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pSLQ7334: pHR-PGK-SV40_NLS-dCas12f-VPR-c- Myc_NLS-mCherry-WPRE

Plasmid

#176268

Purpose

Expression of dCas12f-VPR-mCherry in mammalian cells

Depositor

Stanley Qi

Article

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

dCas12f

Use

Lentiviral

Tags

HA-VPR-mCherry

Expression

Mammalian

Mutation

D326A, D510A

Promoter

PGK

Availability

Academic Institutions and Nonprofits only

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pSLQ9926: pHR-PGK-SV40_NLS-dCasMINI-V4-VPR-c- Myc_NLS-mCherry-WPRE

Plasmid

#176269

Purpose

Expression of dCasMINI-V4-VPR-mCherry in mammalian cells

Depositor

Stanley Qi

Article

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

dCasMINI-V4-VPR-mCherry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Availability

Academic Institutions and Nonprofits only

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pCBh_NLS_hfCas13X(Cas13X_M17YY)_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA

Plasmid

#190033

Purpose

vector for encoding a human codon-optimized High-fidelity Cas13X (hfCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP and mCherry driven by SV40 promoters

Depositor

Huawei Tong

Article

Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

Insert

humanized hfCas13X

Use

Tags

Expression

Mammalian

Mutation

Y672A,Y676A

Promoter

CBh, SV40, hU6

Availability

Academic Institutions and Nonprofits only

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EK0445 SFFV-mCherry-SGc(DNAJB1.s1)cSG-EGFP-(3xMS2) (FLP-IN)

Plasmid

#191155

Purpose

Expression of a sensor for human DNAJB1 (hsp40) 3' UTR in mammalian cells; mCherry marker, EGFP output, three MS2 sequences in 3' UTR

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:DNAJB1.s1:EGFP-3xMS2

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Availability

Academic Institutions and Nonprofits only

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