Addgene

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pGL3-B-pCR

Plasmid

#46369

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Renganathan et al Mol Cancer. 2014 May 23;13:119. doi: 10.

Insert

calretinin promoter (CALB2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mutation

promoter

Promoter

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pEGFP-C2-Cep123-C2 domain

Plasmid

#73329

Purpose

Encodes the distal appendage targeting domain of isoform 1 of Cep123 (residues 500-600) fused to EGFP

Depositor

Michel Bornens

Article

Sillibourne et al Biol Open. 2013 Apr 9;2(6):535-45. doi:

Insert

Cep123 (CEP89 Human)

Use

Tags

EGFP tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 4, 2016

Availability

Academic Institutions and Nonprofits only

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HEL binding scFv D44.1

Plasmid

#111718

Purpose

Yeast Surface Display of D44.1 as a reference starting point for design

Depositor

Sarel Fleishman

Article

Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

Insert

D44.1

Use

Tags

cmyc tag

Expression

Yeast

Mutation

Promoter

Available since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pMADM-alpha

Plasmid

#36890

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP
tdTomato-3Myc
beta-globin intron
Neo
ATG (T)
GFP

Use

Tags

3 Myc tags

Expression

Mammalian

Mutation

deleted nucleotides after nucleotide 274, deleted…

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Available since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pMSCV_PIG_3XHA-Ago1

Plasmid

#170916

Purpose

Expresses 3X-HA-AGO1

Depositor

Constance Ciaudo

Article

Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

Insert

Ago1 (Ago1 Mouse)

Use

Tags

3X-HA

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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3788 pmko-neo A9-1

Plasmid

#8878

Depositor

Stanley Korsmeyer

Article

Wang et al EMBO J. 2005 Jan 26. 24(2):368-81.

Insert

Hox A9 (HOXA9 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only
3788 pmko-neo A9-3

Plasmid

#8880

Depositor

Stanley Korsmeyer

Article

Wang et al EMBO J. 2005 Jan 26. 24(2):368-81.

Insert

Hox A9 (HOXA9 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only
FLAG-SENP2 C548A

Plasmid

#126592

Purpose

Expresses siRNA resistant SENP2 (catalytic dead) in mammalian cells, Dox inducible in TetR cell lines

Depositor

Joanna Morris

Article

Garvin et al Genes Dev. 2019 Mar 1;33(5-6):333-347. d

Insert

SENP2 (SENP2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

C548A

Promoter

CMV

Available since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pMP71-tCD19-llo-mc38tmg

Plasmid

#174603

Purpose

expresses the extracellular and transmembrane domain of murine CD19 with a C terminal fusion of the LLO190 and minigenes of 3 neoantigens from the MC38 tumor in genes ADGPK, DPAGT and Reps1

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

extracell transmem domains moCD19 wCterm fusion LLO190 and minigenes of 3neoantigens MC38tumor in genes ADGPK DPAGT Reps1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_SNORD27h_115mut

Plasmid

#73068

Purpose

expression clone for human mutated SNORD27 (C/D box snoRNA U27)

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

SNORD27 (SNORD27 Human)

Use

Tags

no tag

Expression

Mammalian

Mutation

expression cassette for SNORD27 with AS box from …

Promoter

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only
pGEX4T1-Rpb1-linker

Plasmid

#138468

Purpose

Expresses GST-tagged Rpb1 linker in bacteria

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

Rpb1 (POLR2A Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Amino acids 1460-1585

Promoter

tac

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pQLink-GST-4xCTD

Plasmid

#138471

Purpose

Expresses GST-tagged 4xCTD heptapeptide repeat in bacteria

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

Rpb1 (POLR2A Human)

Use

Tags

GST

Expression

Bacterial

Mutation

4x CTD repeat

Promoter

T5

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAdTrack shALAS-1

Plasmid

#22748

Depositor

Bruce Spiegelman

Article

Estall et al Proc Natl Acad Sci U S A. 2009 Dec 29. 1

Insert

shALAS-1 (Alas1 Mouse)

Use

Adenoviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 14, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA4/TO-mu24-ORF24-2xStrep

Plasmid

#138453

Purpose

Expresses mu24-ORF24 chimera with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

mu24-ORF24 (ORF24 MHV68/KSHV)

Use

Tags

Strep

Expression

Mammalian

Mutation

mu24 amino acids 1-191 fused to ORF24 amino acids…

Promoter

CMV

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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Alt AlphaPS3

Plasmid

#14070

Depositor

Richard Hynes

Article

Stark et al Development. 1997 Nov . 124(22):4583-94.

Insert

Drosphila Alt Alpha PS3 Integrin (scb Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pPSt_35Spr-GFP-t35S

Plasmid

#203592

Purpose

Fully assembled Plant STARR-seq plasmid with the 35S terminator.

Depositor

Stan Fields

Article

Gorjifard et al Nat Commun. 2024 Jul 12;15(1):5868. doi:

Inserts

CaMV 35S promoter + Zm00001d041672 5'UTR
eGFP
CaMV 35S terminator

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pEXP(FLAG/HA-NSP1-K164-H165A)

Plasmid

#188782

Purpose

Expresses FLAG/HA-NSP1-K164-H165A in mammalian cells (SARS-CoV-2 mutant NSP1)

Depositor

Markus Hafner

Article

Anastasakis et al PLoS One. 2024 Jan 26;19(1):e0297262. do

Insert

FLAG/HA-NSP1-K164-H165A (SARS-CoV-2)

Use

Frt site to insert into hek293 t-rex genome

Tags

FLAG/HA

Expression

Mammalian

Mutation

Changed Lysine 164 to Alanine and Histidine 165 t…

Promoter

CMV Dox inducible

Available since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNAintron-CHIKV-Structural polyprotein with Nluc tagged E2

Plasmid

#215699

Purpose

Produces the Chikungunya virus structural polyprotein Capsid-E3-NLucE2-6K-E1

Depositor

Melinda Brindley

Article

Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

Insert

CHIKV structural polyprotein Capsid-E3-NLucE2-6K-E1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pSAVED-CHAT

Plasmid

#214041

Purpose

Bacterial expression plasmid for SAVED-CHAT from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

SAVED-CHAT

Use

Tags

Expression

Bacterial

Mutation

WT

Promoter

lacUV5 promoter

Available since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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KU70-eGFP-SUN1ΔN

Plasmid

#213516

Purpose

Expresses human KU70-eGFP-SUN1ΔN in mammalian cells

Depositor

Karim Mekhail

Article

Shokrollahi et al bioRxiv 2023.05.07.539750

Insert

KU70-eGFP-SUNΔN

Use

Tags

Expression

Mammalian

Mutation

SUN1ΔN has the first 138 amino acid (lamina domai…

Promoter

CMV

Available since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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