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pX459-SpCas9-NG

Plasmid

#171370

Purpose

SpCas9-NG-mediated genome editing.

Depositor

Masahito Ikawa

Article

Oura et al Commun Biol. 2021 Jun 23;4(1):771. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 for sgRNA; CBh for Cas9-2A-puroR

Available since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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AsCas12a

Plasmid

#188492

Purpose

Expresses FLAG tagged AsCas12a

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas12a

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pBABE-puro SHP2 WT

Plasmid

#8329

Depositor

Ben Neel

Article

Zhang et al Mol Cell Biol 2002 Jun;22(12):4062-72.

Insert

SHP2 (PTPN11 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 17, 2005

Availability

Academic Institutions and Nonprofits only

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pLJM60 mUlk1 (wt) barcoded

Plasmid

#48799

Purpose

expresses wt Ulk1

Depositor

David Sabatini

Article

Kang et al Science. 2013 Jul 26;341(6144):1236566.

Insert

ULK1 (Ulk1 Mouse)

Use

Lentiviral

Tags

FLAG and barcode: GGATCC

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 2, 2014

Availability

Academic Institutions and Nonprofits only
pLKO.1-puro U6 sgRNA CAG

Plasmid

#50927

Purpose

U6 driven sgRNA negative control

Depositor

Rene Maehr, Scot Wolfe

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

negative control sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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PB_TRE_sg61-PDGFR_EF1a_eGFP

Plasmid

#186310

Purpose

Plasmid for inducible mammalian cell expression of the sg61 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line construction

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

sg61-PDGFR
eGFP

Use

Tags

His, myc

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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MyoD-pCLBabe

Plasmid

#20917

Depositor

Stephen Tapscott

Article

Yang et al Genes Dev. 2009 Mar 15. 23(6):694-707.

Insert

MyoD (Myod1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
pZR069_CROPseq-sgPTPRC-CRISPRi

Plasmid

#180271

Purpose

Lentiviral CROPseq vector expressing PTPRC (CD45) targeting sgRNA for CRISPRi

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

PTPRC (CD45) sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pMXs-Oct3/4-IP

Plasmid

#15918

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

POU domain, class 5, transcription factor 1 (Pou5f1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

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pScalps_Puro

Plasmid

#99636

Purpose

Empty backbone, 3rd generation lentiviral vector

Depositor

Silvia Monticelli

Article

Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

Type

Empty backbone

Use

Lentiviral

Tags

none

Expression

Mammalian

Mutation

Promoter

SFFVp

Available since

Aug. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pBabe-Puro-RhoA FLARE.sc Biosensor

Plasmid

#12602

Depositor

Klaus Hahn

Article

Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

Insert

RhoA Biosensor (RHOA Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pMSCV-FlagBcl10

Plasmid

#18718

Depositor

Jon Ashwell

Article

Wu et al Proc Natl Acad Sci U S A. 2008 Feb 26. 1

Insert

B cell lymphoma 10 (BCL10 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

July 14, 2008

Availability

Academic Institutions and Nonprofits only
V51 pIRESpuro-GLUE (pGLUE)

Plasmid

#15100

Purpose

tandem-affinity purification plasmid; streptavidin-(streptag) and calmodulin-(CBP) binding affinity tags

Depositor

Randall Moon

Article

Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

Type

Empty backbone

Use

Tags

CBP tag, HA, and streptag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pCEP-Puro-TetOn-L1RP-ORF1_R261A_Halo-GFPai

Plasmid

#202569

Purpose

Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the R261A mutation and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR

Depositor

Liam Holt

Article

Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

Insert

LINE-1

Use

Tags

GFP-AI retrotransposition reporter and HaloTag7 o…

Expression

Mammalian

Mutation

Changed ORF1p Arginine 261 to Alanine

Promoter

Available since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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CLPIT Cry2PHR-mCh-RhoA

Plasmid

#42959

Depositor

David Schaffer

Article

Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

Insert

Cry2PHR-mCherry-RhoA (RHOA Mustard Weed, Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Tet-Off promoter

Available since

April 9, 2013

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-LIC-V5

Plasmid

#120248

Purpose

For LIC cloning with C-terminal V5 tag

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Type

Empty backbone

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

EF1a

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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MCP-mCherry-TIAL1

Plasmid

#207669

Purpose

To express fluorescently labeled MS2 coat binding protein (MCP) fused with RNA binding protein TIAL1

Depositor

Christine Mayr

Article

Horste et al Mol Cell. 2023 Dec 21;83(24):4509-4523.e

Insert

TIAL1 (TIAL1 Human)

Use

Tags

MCP-mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgMSLN

Plasmid

#193588

Purpose

MSLN knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgMSLN (MSLN Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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PB_TRE_Z17-PDGFR_EF1a_mCherry

Plasmid

#186303

Purpose

Plasmid for inducible mammalian cell expression of the Z17 helixCAM and constitutive expression of an identifying fluorescent protein (mCherry). Flanked with PiggyBac sites for easy line construction.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

Z17-PDGFR
mCherry

Use

Tags

HA, myc

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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YSEHPTFTSQY_epitope

Plasmid

#165016

Purpose

Expression of CMV UL83 (363-373) fused to IL-2 signal sequence

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

CMV UL83 (363-373)

Use

Lentiviral

Tags

human IL2 signal sequence at N-term

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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