Addgene

We narrowed to 5,035 results for: pcdna3.1

Showing: 3681 - 3700 of 5035 results

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Vinculin-Clover-CC-Mad-mRuby2

Plasmid

#233239

Purpose

Vinculin FRET tension sensor with coiled-coil Mad domain no vinculin force control

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vinculin-Clover-CC-Mad-mRuby2

Use

Tags

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Vh-Clover-CC-S1-mRuby2-Vt

Plasmid

#233230

Purpose

Vinculin FRET tension sensor with coiled-coil S1 domain

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vh-Clover-CC-S1-mRuby2-Vt

Use

Tags

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Vh-Clover-CC-S0-mRuby2-Vt

Plasmid

#233226

Purpose

Vinculin FRET tension sensor with coiled-coil S0 domain

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vh-Clover-CC-S0-mRuby2-Vt

Use

Tags

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Vh-Clover-CC-S4-mRuby2-Vt

Plasmid

#233228

Purpose

Vinculin FRET tension sensor with coiled-coil S4 domain

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vh-Clover-CC-S4-mRuby2-Vt

Use

Tags

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Vh-Clover-CC-S7-mRuby2-Vt

Plasmid

#233229

Purpose

Vinculin FRET tension sensor with coiled-coil S7 domain

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vh-Clover-CC-S7-mRuby2-Vt

Use

Tags

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Vh-Clover-CC-Mad-mRuby2-Vt

Plasmid

#233232

Purpose

Vinculin FRET tension sensor with coiled-coil Mad domain

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vh-Clover-CC-Mad-mRuby2-Vt

Use

Tags

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Vinculin-Clover-CC-S0-mRuby2

Plasmid

#233233

Purpose

Vinculin FRET tension sensor with coiled-coil S0 domain no vinculin force control

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vinculin-Clover-CC-S0-mRuby2

Use

Tags

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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mproSAAS (21 kDa)

Plasmid

#231634

Purpose

Mammalian expression of the N-terminal domain of the mouse chaperone protein proSAAS-(1-180)

Depositor

Iris Lindberg

Article

Fortenberry et al J Biol Chem. 2002 Feb 15;277(7):5175-86.

Insert

Pcsk1N (Pcsk1n Mouse)

Use

Tags

Expression

Mammalian

Mutation

Deletion of C-terminus (aa181-225)

Promoter

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pTBL435

Plasmid

#226628

Purpose

pcDNA-dTdT

Depositor

Chang Liu

Article

Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

Insert

catalytically deactivated TdT (DNTT Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

D343E, D345E

Promoter

CMV

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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L2383-FRB-GPA-NLuc

Plasmid

#222902

Purpose

Positive control receptor chain for Nanoluciferase signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FRB-GPA-20GS-NanoLuc

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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Cas9-FCPF

Plasmid

#220141

Purpose

Expresses Cas9-FCPF in mammalian cells

Depositor

Xinlai Cheng

Article

Gama-Brambila et al JACS Au. 2021 May 12;1(6):777-785. doi:

Insert

Cas9-FCPF (cas9 Synthetic, S. Pyogenes)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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TRAPa(E162A,M164A,R167A)-3xFLAG-pCDNA

Plasmid

#201964

Purpose

Mammalian expression of mutated Translocon-associated protein subunit alpha

Depositor

Ville Paavilainen

Article

Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

Insert

SSR1 (SSR1 Human)

Use

Tags

3xFLAG-tag

Expression

Mammalian

Mutation

E162A,M164A,R167A

Promoter

Available since

Jan. 4, 2024

Availability

Academic Institutions and Nonprofits only

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TRAPa(ERED198-201AAAA)-3xFLAG-pCDNA

Plasmid

#201965

Purpose

Mammalian expression of mutated Translocon-associated protein subunit alpha

Depositor

Ville Paavilainen

Article

Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

Insert

SSR1 (SSR1 Human)

Use

Tags

3xFLAG-tag

Expression

Mammalian

Mutation

E198A, R199A,E200A, D201A

Promoter

Available since

Jan. 4, 2024

Availability

Academic Institutions and Nonprofits only

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TRAPa(RKRKR232-236AAAAA)-3xFLAG-pCDNA

Plasmid

#201967

Purpose

Mammalian expression of mutated Translocon-associated protein subunit alpha

Depositor

Ville Paavilainen

Article

Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

Insert

SSR1 (SSR1 Human)

Use

Tags

3xFLAG-tag

Expression

Mammalian

Mutation

R232A, K233A, R234A, K235A, R236A

Promoter

Available since

Jan. 4, 2024

Availability

Academic Institutions and Nonprofits only

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TRAPa(W255A,N262E,K266E)-3xFLAG-pCDNA

Plasmid

#201968

Purpose

Mammalian expression of mutated Translocon-associated protein subunit alpha

Depositor

Ville Paavilainen

Article

Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

Insert

SSR1 (SSR1 Human)

Use

Tags

3xFLAG-tag

Expression

Mammalian

Mutation

W255A,N262E,K266E

Promoter

Available since

Jan. 4, 2024

Availability

Academic Institutions and Nonprofits only

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CCM2_I432D_eGFP

Plasmid

#208879

Purpose

Express eGFP-CCM2 with the I432D mutation in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Neil J. et al bioRxiv

Insert

CCM2 (CCM2 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

mutation I432D

Promoter

Available since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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TLNRD1_35-345_eGFP

Plasmid

#208881

Purpose

Express eGFP-TLNRD1 lacking N and Cterm domains (TLNRD1 amino acids 35-345) in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Neil J. et al bioRxiv

Insert

TLNRD1 (TLNRD1 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

amino acid sequence 35-345

Promoter

Available since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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TLNRD1_5H_eGFP

Plasmid

#208861

Purpose

Express eGFP-TLNRD1 5 helix bundle (TLNRD1 lacking amino acids 142-273) in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Neil J. et al bioRxiv

Insert

TLNRD1 5 helix bundle (TLNRD1 Human)

Use

Tags

Expression

Mammalian

Mutation

Deleted amino acid 142-273

Promoter

Available since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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CCM2_wt_eGFP

Plasmid

#208876

Purpose

Express eGFP-CCM2 in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Neil J. et al bioRxiv

Insert

CCM2 (CCM2 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

WT sequence

Promoter

Available since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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CCM2_I428S_eGFP

Plasmid

#208878

Purpose

Express eGFP-CCM2 with the I428S mutation in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Neil J. et al bioRxiv

Insert

CCM2 (CCM2 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

mutation I428S

Promoter

Available since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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