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pEGFP-N1-mSNX27 RNAi-resistant

Plasmid

#163620

Purpose

mouse SNX27 cDNA with point mutation to resist siRNA against SNX27 C-terminally tagged with GFP

Depositor

Josef Kittler

Article

Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

Insert

mouse SNX27 RNAi-resistant (Snx27 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

RNAi resistance

Promoter

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pPRISM-Stop-gcry-eGFP

Plasmid

#117773

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

3x3 Stop sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP ratAPOBEC1 L173A/G227A

Plasmid

#167169

Purpose

Expresses rat APOBEC1 L173A/G227A in mammalian cells. Please note that this does not express GFP.

Depositor

Silvestro Conticello

Article

Chieca et al bioRxiv 2021

Insert

APOBEC1 (Apobec1 Rat)

Use

Tags

Expression

Mammalian

Mutation

changed Leucine 173 to Alanine; changed Glycine 2…

Promoter

CMV promoter

Available since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pT2KXIGdeltaIn-MCS-birA-EGFP

Plasmid

#58377

Purpose

tol2 transgenesis for biotin based TRAP - BirA ligase

Depositor

Didier Stainier

Article

Housley et al Development. 2014 Oct;141(20):3988-93. d

Insert

birA EGFP

Use

Tags

EGFP

Expression

Mutation

Promoter

Available since

Aug. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pLL5.0 hCortKD-Flcortmut3YF EGFP

Plasmid

#187263

Purpose

Lentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YF mutant

Depositor

Alpha Yap

Article

Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

Insert

Cortactin (CTTN Human)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

3YF (Y412, Y470, Y486)

Promoter

U6, 5'LTR

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pSMPUW_6.19_Ex_Ef1a-EGFP-HA-SV40-Zeo

Plasmid

#128455

Purpose

lentiviral expression vector with Ef1a promoter, EGFP gene, C-terminal HA tag, SV40 promoter, and Zeo Resistance gene

Depositor

Lindy Barrett

Insert

Ef1a promoter, EGFP gene, C-terminal HA tag, SV40 promoter, and Zeocin Resistance gene

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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3xEGFP-mMeikin (E156R, R159E)

Plasmid

#174710

Purpose

Mammalian expression of mouse Meikin with Separase-resistant mutations

Depositor

Iain Cheeseman

Article

Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

Insert

Meikin (Meikin Mouse)

Use

Tags

3xEGFP

Expression

Mammalian

Mutation

E156R, R159E

Promoter

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pRVdG-EGFP-N-P-M-nucBFP-SynPhRFP-L

Plasmid

#52478

Purpose

Expresses EGFP, nuclear mTagBFP, and Synaptophysin-TagRFP-T

Depositor

Sebastian Seung

Article

Wickersham et al Nat Commun. 2013;4:2332. doi: 10.1038/nc

Inserts

EGFP
nuclear mTagBFP
Synaptophysin-TagRFP-T

Use

Rabies virus

Tags

TagRFP-T

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pMY-MCS-GSG-eGFP

Plasmid

#163350

Purpose

Retroviral backbone for in-frame cloning of a C-terminally GFP labeled gene of interest.

Depositor

Louis Ates, Teunis Geijtenbeek

Article

van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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PLK3 335-646-pEGFP

Plasmid

#23267

Depositor

Wei Dai

Article

Yang et al Cancer Res. 2008 Jun 1. 68(11):4077-85.

Insert

PLK3 (PLK3 Human)

Use

Tags

pEGFP

Expression

Mammalian

Mutation

aa 335-646

Promoter

Available since

Dec. 20, 2012

Availability

Academic Institutions and Nonprofits only
pBSU6-shTim23/CMV-eGFP

Plasmid

#89795

Purpose

Expresses shRNA against TIM23 with a separate promoter for expression of GFP.

Depositor

Robert Friedlander

Article

Yano et al Nat Neurosci. 2014 Jun;17(6):822-31. doi

Insert

shRNA Tim23 (Timm23 Mouse)

Use

Tags

eGFP under separate promoter

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C3-PLK4-S305E-3xFLAG

Plasmid

#69840

Purpose

This plasmid encodes PLK4 isoform 1 carrying an S305E mutation with an N-terminal EGFP tag and C-terminal 3xFLAG tag

Depositor

Michel Bornens

Article

Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

Insert

PLK4 (PLK4 Human)

Use

Tags

3xFLAG tag and EGFP

Expression

Mammalian

Mutation

S305E

Promoter

CMV

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-zebrafish lyn–GFP

Plasmid

#35956

Depositor

Anna Huttenlocher

Article

Yoo et al Nature. 2011 Nov 20;480(7375):109-12. do

Insert

humanized zebrafish lyn (lyn Zebrafish)

Use

Tags

EGFP

Expression

Mammalian

Mutation

wt

Promoter

cmv

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-FOXL2-MUT

Plasmid

#194585

Purpose

Mammalian Expression of mEGFP-FOXL2 Mutant (A328Pfs*28) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

FOXL2 (FOXL2 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

A328Pfs*28

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-eGFP-OPTN S513A

Plasmid

#175764

Purpose

Lentiviral vector expressing N-terminally tagged eGFP-OPTN S513A

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

OPTN (OPTN Human)

Use

Lentiviral

Tags

Expression

Mutation

S513A

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pMXs-IP-EGFP-ULK2(K39T)

Plasmid

#38206

Depositor

Noboru Mizushima

Article

Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

Insert

Unc-51 like kinase 2 (Ulk2 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

changed Lysine 39 to Threonine

Promoter

Available since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only
pT2-cryR;clecl14aCE1-P1Egfp

Plasmid

#90157

Purpose

contains clec14a enhancer sequence, and clec14a specific gene promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

clec14aE1P1Egfp

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFPN1-N-gamma-tubulin (1-333)

Plasmid

#87858

Purpose

Fluorescent fragment of gamma-tubulin that lacks gamma-tubulin DNA binding domain and NLS

Depositor

Maria Alvarado-Kristensson

Article

Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

Insert

gamma-tubulin 1 (TUBG1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Fragment from aa 1 to 333

Promoter

Available since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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KP201: pMVP (L5-L4) eGFP-P2A

Plasmid

#121708

Purpose

pMVP L5-L4 entry plasmid, contains eGFP-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term eGFP linked by P2A to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eGFP-P2A (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HseIF4E-F47AI79A_Z

Plasmid

#148136

Purpose

Mammalian Expression of HseIF4E-F47AI79A

Depositor

Elisa Izaurralde

Insert

HseIF4E-F47AI79A (EIF4E Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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