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Plasmid#53241Purposeexpress yellow fluorescent protein (YFP) with strong T7 RBS at a low gene dosage (3-4 copies/cell)DepositorInsertYFP
UseTagsExpressionBacterialMutationPromoterPLtetO1Available sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET12a-AL-09 FL C214S
Plasmid#47086DepositorInsertAL-09 FL C214S (IGKV3-20 Human)
UseTagsompTExpressionBacterialMutationC214SPromoterT7Available sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV-mTagBFP2-FRB-Rab5
Plasmid#214272PurposeMammalian expression of mTagBFP2-FRB-Rab5 (early endosome marker)DepositorArticleInsertmTagBFP2-FRB-Rab5A (RAB5A Canis lupus familiaris (Dog))
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-mTagBFP2-FRB-Rab7
Plasmid#214273PurposeMammalian expression of mTagBFP2-FRB-Rab7 (late endosome marker)DepositorArticleInsertmTagBFP2-FRB-Rab7A (RAB7A Canis lupus familiaris (Dog))
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMB-LEr
Plasmid#61186PurposeFluorescent reporter for late endosome mCherry-MtRAB5A2 expressed under MtBCP1 promoterDepositorInsertMtRAB5A2
UseTagsmCherryExpressionPlantMutationPromoterMtBCP1Available sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-RAB5A-IRES-Puromycin
Plasmid#134858PurposeExpresses EGFP-tagged early endosome markerDepositorInsertRas-related protein 5A
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
Pet15b-Internalization Control
Plasmid#186553PurposeNegative Control mediating endosomal localizationDepositorInsert10R-8His-EGFP
UseTags8xHisExpressionBacterialMutationPromoterT7Available sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLEX-FKBP-Rab7a-Blasticidin
Plasmid#120716PurposeExpresses late endosome-targeted recruiter protein in mammalian cells & for virus productionDepositorInsertRab7a
UseLentiviralTagsFKBP and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pB80-FKBP-mCherry-RAB11
Plasmid#174622PurposeRapalog-inducible coupling to recycling endosomes (RAB11) via FKBPDepositorInsertFKBP-mCherry-RAB11 (RAB11A Human, Synthetic)
UseTagsFKBP-mCherryExpressionMammalianMutationmCherry: Met1Del; RAB11: Met1DelPromoterChicken beta-actinAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
TPC1-G-GECO1.2
Plasmid#207143PurposeFusion of Ca2+ reporter and endosomal Ca2+-permeable channelDepositorInsertTPCN1 (TPCN1 Human)
UseTagsG-GECO1.2ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET12a-AL-09 FL
Plasmid#47081DepositorInsertAL-09 (IGKV3-20 Human)
UseTagsompTExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-hS + eGFP
Plasmid#74945PurposeDosage control and tracing of reprogramming episomesDepositorInsertSox2 (SOX2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pB80-iLID-mCherry-RAB5
Plasmid#174624PurposeOptogenetic coupling to early endosomes (RAB5) via iLIDDepositorInsertiLID-mCherry-RAB5 (RAB5A Human, Synthetic)
UseTagsiLID-mCherryExpressionMammalianMutationmCherry: Met1Del; RAB5: Met1DelPromoterChicken beta-actinAvailable sinceNov. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pB80-iLID-mCherry-RAB11
Plasmid#174623PurposeOptogenetic coupling to recycling endosomes (RAB11) via iLIDDepositorInsertiLID-mCherry-RAB11 (RAB11A Human, Synthetic)
UseTagsiLID-mCherryExpressionMammalianMutationmCherry: Met1Del; RAB11: Met1DelPromoterChicken beta-actinAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
mOrange2-Endo-14
Plasmid#57955PurposeLocalization: Endosomes, Excitation: 549, Emission: 565DepositorInsertEndo (RHOB Human)
UseTagsmOrange2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET12a-AL-103 Y91F
Plasmid#47093DepositorInsertAL-103 Y91F (IGKV3-20 Human)
UseTagsompTExpressionBacterialMutationY91FPromoterT7Available sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET12a-AL-09 FL H87Y C214S
Plasmid#47087DepositorInsertAL-09 FL H87Y C214S (IGKV3-20 Human)
UseTagsompTExpressionBacterialMutationH87Y C214SPromoterT7Available sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
FRB-mCherry-EEA1
Plasmid#222419PurposeTo express an early endosome "anchor" for rapamycin-induced heterodimerisation in mammalian cellsDepositorInsertEEA1 (EEA1 Human)
UseTagsFRB-mCherryExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-pADH3-Snf7-4V5-APEX2-URA
Plasmid#207065PurposeModerate overexpression of Snf7-4V5-APEX2 under ADH3 promoter. Marker for late endosomes. Only partially functional.DepositorInsertSnf7
UseTags4xV5-APEX2ExpressionYeastMutationPromoterpADH3Available sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
SEC7-2EGFP-ter-NAT
Plasmid#184764PurposeMarker for yeast late Golgi/early endosome . Integration of 2xGFP tag at SEC7 C terminus. Uses antibiotic resistance marker natMX6.DepositorInsertSEC7 (SEC7 Budding Yeast)
UseTags2EGFPExpressionYeastMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
SEC7-2Katushka-ter-Hygromycin
Plasmid#184772PurposeRed marker for yeast late Golgi/early endosome. Integration of 2xKatushka tag at SEC7 C terminus. Uses antibiotic resistance marker hphMX6.DepositorInsertSEC7 (SEC7 Budding Yeast)
UseTags2KatushkaExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET12a-AL-103 Y96F
Plasmid#47094DepositorInsertAL-103 Y96F (IGKV3-20 Human)
UseTagsompTExpressionBacterialMutationY96FPromoterT7Available sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET12a-AL-103 Y32F
Plasmid#47091DepositorInsertAL-103 Y32F (IGKV3-20 Human)
UseTagsompTExpressionBacterialMutationY32FPromoterT7Available sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET12a-AL-09 FL Q42K C214S
Plasmid#47089DepositorInsertAL-09 FL Q42K C214S (IGKV3-20 Human)
UseTagsompTExpressionBacterialMutationQ42K C214SPromoterT7Available sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET12a-AL-09 FL I34N C214S
Plasmid#47088DepositorInsertAL-09 FL I34N C214S (IGKV3-20 Human)
UseTagsompTExpressionBacterialMutationI34N C214SPromoterT7Available sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET12a-AL-09 Y96F
Plasmid#47085DepositorInsertAL-09 Y96F (IGKV3-20 Human)
UseTagsompTExpressionBacterialMutationY96FPromoterT7Available sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET12a-AL-09 Y91F
Plasmid#47084DepositorInsertAL-09 Y91F (IGKV3-20 Human)
UseTagsompTExpressionBacterialMutationY91FPromoterT7Available sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET12a-AL-09 Y32F Y96F
Plasmid#47083DepositorInsertAL-09 Y32F Y96F (IGKV3-20 Human)
UseTagsompTExpressionBacterialMutationY32F Y96FPromoterT7Available sinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET12a-AL-09 Y32F
Plasmid#47082DepositorInsertAL-09 Y32F (IGKV3-20 Human)
UseTagsompTExpressionBacterialMutationY32FPromoterT7Available sinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-RAB11B-IRES-Puromycin
Plasmid#134860PurposeExpresses EGFP-tagged recycling endosome markerDepositorInsertRAB11B (RAB11B Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZJOM44
Plasmid#133653PurposeIntegration of SNF7-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late endosome.DepositorInsertp1k-SNF7-mCherry (SNF7 Budding Yeast)
UseTagsmCherryExpressionYeastMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZJOM73
Plasmid#133651PurposeIntegration of CHS5-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late golgi/early endosome.DepositorInsertp1k-CHS5-mCherry (CHS5 Budding Yeast)
UseTagsmCherryExpressionYeastMutationPromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZJOM40
Plasmid#133650PurposeIntegration of SEC7-DuDre as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late golgi/early endosome.DepositorInsertp1k-SEC7-DuDre (SEC7 Budding Yeast)
UseTagsDuDreExpressionYeastMutationPromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRK5-FLAG-C7orf59
Plasmid#42332DepositorInsertC7orf59 (LAMTOR4 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV, SP6Available sinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRosetta_v2
Plasmid#136477PurposeLentiviral GFP Reporter assay for determining appropriate dose of puromycin, blasticidin, and hygromycinDepositorInsertHygroR-T2A-BlastR-P2A-PuroR-F2A-EGFP
UseLentiviralTagsExpressionMutationPromoterPGKAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFEndoNA
Plasmid#174724PurposeExpresses GFP-endoNA fusion protein for degradation of polysialic acid. The endoNA is a catalytically active endosialidase from bacteriophage PK1A.DepositorInsertGFP-endoNA fusion protein
UseTagsHistagExpressionBacterialMutationPromoterT5Available sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX-FKBP-Rab11-Blasticidin
Plasmid#120717PurposeExpresses recycling endosome-targeted recruiter protein in mammalian cells & for virus productionDepositorInsertRab11a
UseLentiviralTagsFKBP and mCherryExpressionMammalianMutationAmino acids 2-216PromoterCMVAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMCSF-R-luc
Plasmid#12420DepositorInsertM-CSF receptor promoter fragment (CSF1R Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
PKG I alpha dNPS
Plasmid#16391DepositorInsertPKG1 alpha (PRKG1 Human)
UseTagsHAExpressionMammalianMutationN-terminal pseudosubstrate domain deletion: delet…PromoterAvailable sinceApril 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pHR-FKBP:mCherry-Rab7a
Plasmid#72903PurposeLate endosome-targeted mCherry-tagged recruiter constructDepositorInsertRab7a (RAB7A Human)
UseLentiviralTagsFKBP, mCherryExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only