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pCMV-Frankenbody-FLAG-Halo
Plasmid#175296PurposeTrack mature and nascent FLAG tagged proteins in live cellsDepositorInsertanti-FLAG frankenbody fused with HaloTag
UseTagsHaloTagExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pB80-hKif5b(1-560)rigor-2xmNeongreen
Plasmid#174649PurposeLive-cell marker for stable microtubulesDepositorInserthKif5B(1-560)rigor-2xmNeongreen (KIF5B Human)
UseTagsmNeongreen-mNeongreenExpressionMammalianMutationrigor mutation G234APromoterChicken beta-actinAvailable sinceMarch 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGRG36-Kn-PA1-GFP
Plasmid#79088PurposepGRG36-Kn-PA1-GFP is a Kanamycine resistance plasmid used to integrate a GFP expression cassette into the chromosome of E.coli, described in Yang et al. 2016 (Pending publication Pubmed ID)DepositorInsertGFP
UseBacterial chromosome integration and expression o…TagsExpressionBacterialMutationPromoterPA1Available sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMP71-tCD19-mGFP
Plasmid#174609Purposeexpresses membrane-associated (palmitoylated) EGFP and extracellular and transmembrane domains of murine CD19DepositorInsertCD19 extracellular and transmembrane domain (Cd19 Mouse)
UseTagspalmitoylated EGFPExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAdTrack-CMV HA ALAS-1
Plasmid#22746DepositorInsertALAS-1 (Alas1 Mouse)
UseAdenoviralTagsHAExpressionMammalianMutationPromoterAvailable sinceJune 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
pWPI-N1ICD
Plasmid#185525PurposeConstitutive overexpression of human NOTCH1 intracellular domainDepositorInsertNOTCH1 intracellular domain (NOTCH1 Human)
UseLentiviralTagsExpressionMutationPromoterEF-1aAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA C12 ecDHFR DD YFP HA
Plasmid#192815PurposeExpresses an N-terminal enhanced destabilizing domain (DD) version of E. coli DHFR with higher basal turnover in mammalian systems. Contains missense mutations W74R/T113S/E120D/Q146L.DepositorInsertE. coli dihydrofolate reductase
UseTagsenhanced yellow fluorescent protein and hemagglut…ExpressionMammalianMutationW74R/T113S/E120D/Q146LPromoterCMVAvailable sinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) Flag-His-ATM kd
Plasmid#31986DepositorInsertATM kd (ATM Human)
UseTagsFlag and HisExpressionMammalianMutation3'UTRf 9 nucl/D2870A N2875k. catalytically …PromoterAvailable sinceMarch 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFB2F
Plasmid#195289PurposeBicistronic HSV-TK and mScarlet resistance for positive/negative selectionDepositorInsertHSV-TK 2A mScarlet
UseTagsExpressionMammalianMutationPromoterPGKAvailable sinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX601-GFP
Plasmid#84040PurposeStaphylococcus aureus (SaCas9) conjugated with GFPDepositorInsertSaCas9
UseAAV and CRISPRTagsNLS and T2A-EGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV-TetO-hNGN2-Puro
Plasmid#79049PurposeExpresses human NEUROGENIN2 (hNGN2) and puromycin resistance gene under control of TetON promoter. This lentiviral vector is used to generate NGN2-iNs (induced neurons) from hiPSCs and hiPSC-NPCs.DepositorInserthNGN2-P2A-PuroR (NEUROG2 Human)
UseLentiviralTagsExpressionMutationPromoterTetOAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
TccCas13a Twin SUMO
Plasmid#199754PurposeExpresses codon optimized thermostabile TccCas13a in E.coliDepositorInsertTccCas13a
UseTagsSUMOExpressionBacterialMutationPromoterLacIAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Frankenbody-FLAG-mRuby2
Plasmid#175294PurposeTrack mature and nascent FLAG tagged proteins in live cellsDepositorInsertanti-FLAG frankenbody fused with mRuby2
UseTagsmRuby2ExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCWori cytochrome P450-BM3h 9D7
Plasmid#61308PurposeExpresses Cytochrome P450-BM3h 9D7 with C-terminal 6-His tagDepositorInsertCytochrome P450-BM3 heme domain 9D7
UseTags6xHisExpressionBacterialMutationT268A, I263A, T438V, A328GPromoterTacAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSP6-RNAP
Plasmid#48143PurposeShuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage SP6 under control of the xylose inducible promoter PxylADepositorInsertrna polymerase SP6
UseSynthetic BiologyTagsExpressionBacterialMutationV314A (numbering based on NCBI Sequence ID: NP_85…PromoterAvailable sinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pX601-mCherry
Plasmid#84039PurposeStaphylococcus aureus (SaCas9) conjugated with mCherryDepositorInsertSaCas9
UseAAV and CRISPRTagsNLS and T2A-mCherryExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMSCVpuro-DEST
Plasmid#119745PurposeAllows Gateway cloning of gene of interest into retroviral pMSCV vector (with Puromycin resistance)DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
5HT2C-RNA2-Flag
Plasmid#79679Purposeserotonin receptor 2C isoform 2 with C-terminal Flag tag, non-edited isoformDepositorInsertserotonin receptor 2C, RNA2 (HTR2C Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only
